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EC Number Protein Variants Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.139D192A site-directed mutagenesis, the mutation has no effect on the enzyme activity 732167
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.139D206A site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme 732167
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.139D215A 15fold decrease in catalytic efficiency 738687
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.139D274A as active as wild-type enzyme -, 646735
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.139D332A site-directed mutagenesis, inactive mutant 732167
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.139D335A complete loss of activtiy -, 738687
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.139D335A weak binding of substrate -, 738687
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.139D364A 0.000015% of wild-type activity -, 646735
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.139D364A/E392C 0.0000004% of wild-type activity 646735
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.139D365A activity is 0.0001% of wild-type activity 646737
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