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Information on EC 3.1.3.75 - phosphoethanolamine/phosphocholine phosphatase and Organism(s) Gallus gallus and UniProt Accession O73884

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IUBMB Comments
Requires active site Mg2+ but also works, to a lesser extent, with Co2+ and Mn2+. The enzyme is highly specific for phosphoethanolamine and phosphocholine.
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This record set is specific for:
Gallus gallus
UNIPROT: O73884
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The taxonomic range for the selected organisms is: Gallus gallus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
phospho1, phosphorylcholine phosphatase, atpecp1, phosphoethanolamine/phosphocholine phosphatase, phosphatase phospho1, 3x11a, phospho1-3a, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PHOSPHO1
-
-
additional information
PHOSPHO1 is a member of the haloacid dehalogenase superfamily of hydrolases
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
phosphoethanolamine phosphohydrolase
Requires active site Mg2+ but also works, to a lesser extent, with Co2+ and Mn2+. The enzyme is highly specific for phosphoethanolamine and phosphocholine.
CAS REGISTRY NUMBER
COMMENTARY hide
52227-92-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
O-phosphoethanolamine + H2O
ethanolamine + phosphate
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
lansoprazole
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lansoprazole
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1 mM decreases calcifying potential in the presence of phosphoethanolamine by 10%
SCH202676
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1 mM decreases calcifying potential in the presence of phosphoethanolamine by 10%
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ascorbic acid
-
increases PHOSPHO1 expression in matrix vesicles after 12 days in culture
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.1
sequence calculation
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
from 3 weeks old male chickens, from tibia, diaphyseal cortical and trabecular bone, expression pattern, PHOSPHO1 is specifically expressed at sites of mineralization in bone and cartilage
Manually annotated by BRENDA team
from 17 days old embryonic chickens, PHOSPHO1 gene expression
Manually annotated by BRENDA team
from 3 weeks old male chickens, from tibia, growth plate cartilage, expression pattern, PHOSPHO1 is specifically expressed at sites of mineralization in bone and cartilage
Manually annotated by BRENDA team
from growth plates of proximal tibiotarsi, 3X11A expression at high levels in differentiating growth plate chondrocytes, 3X11A expression is upregulated 5fold during chondrocyte terminal differentiation and its expression is approximately 100fold higher in hypertrophic chondrocytes than in non-chondrogenic tissues
Manually annotated by BRENDA team
-
mineralization by PHOSPHO1 in medullary bone
Manually annotated by BRENDA team
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PHOSPHO1 expression occurs prior to E6.5 and is initially restricted to the bone collar within the mid-shaft of the diaphysis of long bones. By E11.5, expression is observed over the entire length of the diaphysis
Manually annotated by BRENDA team
additional information
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PHOP1_CHICK
268
0
30443
Swiss-Prot
other Location (Reliability: 4)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28600
x * 33000, recombinant PHOSPHO1, SDS-PAGE, x * 28600, x * 30400, alternative transcripts for PHOSPHO1 in growth cartilage, Western blot analysis
30400
x * 33000, recombinant PHOSPHO1, SDS-PAGE, x * 28600, x * 30400, alternative transcripts for PHOSPHO1 in growth cartilage, Western blot analysis
30442
x * 30442, sequence calculation
33000
x * 33000, recombinant PHOSPHO1, SDS-PAGE, x * 28600, x * 30400, alternative transcripts for PHOSPHO1 in growth cartilage, Western blot analysis
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant PHOSPHO1
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
from growth plate chondrocytes, expression in Escherichia coli TOP10
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Houston, B.; Seawright, E.; Jefferies, D.; Hoogland, E.; Lester, D.; Whitehead, C.; Farquharson, C.
Identification and cloning of a novel phosphatase expressed at high levels in differentiating growth plate chondrocytes
Biochim. Biophys. Acta
1448
500-506
1999
Gallus gallus (O73884)
Manually annotated by BRENDA team
Houston, B.; Stewart, A.J.; Farquharson, C.
PHOSPHO1-a novel phosphatase specifically expressed at sites of mineralisation in bone and cartilage
Bone
34
629-637
2004
Homo sapiens, Gallus gallus (O73884)
Manually annotated by BRENDA team
Stewart, A.J.; Roberts, S.J.; Seawright, E.; Davey, M.G.; Fleming, R.H.; Farquharson, C.
The presence of PHOSPHO1 in matrix vesicles and its developmental expression prior to skeletal mineralization
Bone
39
1000-1007
2006
Gallus gallus
Manually annotated by BRENDA team
Roberts, S.; Narisawa, S.; Harmey, D.; Millan, J.L.; Farquharson, C.
Functional involvement of PHOSPHO1 in matrix vesicle-mediated skeletal mineralization
J. Bone Miner. Res.
22
617-627
2007
Gallus gallus, Homo sapiens, Mus musculus
Manually annotated by BRENDA team
MacRae, V.E.; Davey, M.G.; McTeir, L.; Narisawa, S.; Yadav, M.C.; Millan, J.L.; Farquharson, C.
Inhibition of PHOSPHO1 activity results in impaired skeletal mineralization during limb development of the chick
Bone
46
1146-1155
2010
Gallus gallus (O73884)
Manually annotated by BRENDA team