Information on EC 3.1.3.100 - thiamine phosphate phosphatase

for references in articles please use BRENDA:EC3.1.3.100
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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
3.1.3.100
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RECOMMENDED NAME
GeneOntology No.
thiamine phosphate phosphatase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
thiamine phosphate + H2O = thiamine + phosphate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
thiamine diphosphate biosynthesis III (Staphylococcus)
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thiamine salvage IV (yeast)
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thiamine diphosphate biosynthesis IV (eukaryotes)
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Thiamine metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
thiamine phosphate phosphohydrolase
The enzyme participates in the thiamine biosynthesis pathway in eukaryotes and a few prokaryotes. These organisms lack EC 2.7.4.16, thiamine-phosphate kinase, and need to convert thiamine phosphate to thiamine diphosphate, the active form of the vitamin, by first removing the phosphate group, followed by diphosphorylation by EC 2.7.6.2, thiamine diphosphokinase.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
thiamine + phosphate
thiamine phosphate + H2O
show the reaction diagram
thiamine diphosphate + H2O
thiamine phosphate + phosphate
show the reaction diagram
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preferred substrate
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-
?
thiamine phosphate + H2O
thiamine + phosphate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
thiamine + phosphate
thiamine phosphate + H2O
show the reaction diagram
thiamine diphosphate + H2O
thiamine phosphate + phosphate
show the reaction diagram
-
preferred substrate
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-
?
thiamine phosphate + H2O
thiamine + phosphate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
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membrane-bound enzyme activity is enhanced 1.7fold by 5 mM Mg2+, while the soluble enzyme form is independent from Mg2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
levamisole
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membrane-bound enzyme is strongly inhibited by levamisole in uncompetitive manner while the soluble enzyme form is insensitive to levamisole
thiamine
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the enzyme is repressed completely by excess thiamine (0.01 mM)
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.036
thiamine diphosphate
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at pH 5.0, temperature not specified in the publication
0.049 - 0.7
thiamine phosphate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
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optimum around pH 4.0
6
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soluble enzyme form
9
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membrane enzyme form
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
17800
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x * 17800, soluble enzyme form, SDS-PAGE
24000
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2 * 24000, SDS-PAGE
56000
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x * 56000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
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2 * 24000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55
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the enzyme is inactivated after 10 min at 55°C
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DEAE-cellulose column chromatography
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Toyopearl HW-55 column chromatography and gel filtration
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli JA 221 cells
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the enzyme activity is decreased when the seedlings are grown under the illumination
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