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Ligand thiamine phosphate

Basic Ligand Information

Molecular Structure

Related pathways

Pathway Source

Pathways

BRENDA

vitamin B1 metabolism

KEGG

Biosynthesis of cofactors, Thiamine metabolism

MetaCyc

thiamine diphosphate biosynthesis I (E. coli), thiamine diphosphate biosynthesis II (Bacillus), thiamine diphosphate biosynthesis III (Staphylococcus), thiamine diphosphate biosynthesis IV (eukaryotes), thiamine diphosphate salvage I, thiamine diphosphate salvage II, thiamine diphosphate salvage IV (yeast), thiamine phosphate formation from pyrithiamine and oxythiamine (yeast) more

Show all BRENDA pathways known for thiamine phosphate

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Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (5 results)

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Show Natural Substrate (5 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.7.4.16

ATP + thiamine phosphate = ADP + thiamine diphosphate

642545, 642546, 661335, 690944, 738905, 761512, 762462

3C9T, 3D-view

2.7.4.16

ATP + thiamine phosphate = ADP + thiamine diphosphate

642545, 642546, 661335, 690944, 738905, 761512, 762462

5DD7, 3D-view

2.7.4.16

ATP + thiamine phosphate = ADP + thiamine diphosphate

642545, 642546, 661335, 690944, 738905, 761512, 762462

8YKU, 3D-view

2.7.4.16

3 entries

3.1.3.100

thiamine phosphate + H2O = thiamine + phosphate

737016, 735988, 735989, 735723, 737294, 750683, 751961, 752316

-

3.1.3.2

thiamine phosphate + H2O = thiamine + phosphate

717266

-

In Vivo Product in Enzyme-catalyzed Reactions (24 results)

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Show Natural Product (24 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole = thiamine monophosphate + diphosphate

-

1G4S, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole = thiamine monophosphate + diphosphate

-

2TPS, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole = thiamine monophosphate + diphosphate

-

3NL3, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole = thiamine monophosphate + diphosphate

-

3NL6, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole = thiamine monophosphate + diphosphate

-

3NM3, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + (R,Z)-2-(2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate = diphosphate + thiamine phosphate + CO2

-

1G4S, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + (R,Z)-2-(2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate = diphosphate + thiamine phosphate + CO2

-

2TPS, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + (R,Z)-2-(2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate = diphosphate + thiamine phosphate + CO2

-

3NL3, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + (R,Z)-2-(2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate = diphosphate + thiamine phosphate + CO2

-

3NL6, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + (R,Z)-2-(2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate = diphosphate + thiamine phosphate + CO2

-

3NM3, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate = diphosphate + thiamine phosphate + CO2

-

1G4S, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate = diphosphate + thiamine phosphate + CO2

-

2TPS, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate = diphosphate + thiamine phosphate + CO2

-

3NL3, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate = diphosphate + thiamine phosphate + CO2

-

3NL6, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate = diphosphate + thiamine phosphate + CO2

-

3NM3, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate

-

1G4S, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate

-

2TPS, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate

-

3NL3, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate

-

3NL6, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate

-

3NM3, 3D-view

2.7.1.89

ATP + thiamine = ADP + thiamine monophosphate

642072

-

2.7.1.89

ATP + thiamine = ADP + thiamine phosphate

-

-

3.1.3.100

thiamine + phosphate = thiamine phosphate + H2O

-

-

3.1.3.100

thiamine diphosphate + H2O = thiamine phosphate + phosphate

-

-

2.5.1.3

20 entries

2.7.1.89

2 entries

3.1.3.100

2 entries

Substrate in Enzyme-catalyzed Reactions (14 results)

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Show Substrate (14 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.5.1.2

thiamine phosphate + aniline = 2-(4-methyl-1,3-thiazol-5-yl)ethyl dihydrogenphosphate + 5-(anilinomethyl)-2-methylpyrimidin-4-amine

636860

-

2.7.4.16

ADP + thiamine phosphate = ?

642545

3C9T, 3D-view

2.7.4.16

ADP + thiamine phosphate = ?

642545

5DD7, 3D-view

2.7.4.16

ADP + thiamine phosphate = ?

642545

8YKU, 3D-view

2.7.4.16

ATP + thiamine phosphate = ADP + thiamine diphosphate

642545, 642546, 661335, 690944, 738905, 761512, 762462

3C9T, 3D-view

2.7.4.16

ATP + thiamine phosphate = ADP + thiamine diphosphate

642545, 642546, 661335, 690944, 738905, 761512, 762462

5DD7, 3D-view

2.7.4.16

ATP + thiamine phosphate = ADP + thiamine diphosphate

642545, 642546, 661335, 690944, 738905, 761512, 762462

8YKU, 3D-view

2.7.4.16

ITP + thiamine phosphate = ?

642545

3C9T, 3D-view

2.7.4.16

ITP + thiamine phosphate = ?

642545

5DD7, 3D-view

2.7.4.16

ITP + thiamine phosphate = ?

642545

8YKU, 3D-view

2.7.4.16

9 entries

3.1.3.100

thiamine phosphate + H2O = thiamine + phosphate

737016, 735988, 735989, 735723, 737294, 750683, 751961, 752316

-

3.1.3.2

thiamine monophosphate + H2O = thiamine + phosphate

715966

-

3.1.3.2

thiamine phosphate + 2 H2O = thiamine + phosphate

775351

-

3.1.3.2

thiamine phosphate + H2O = thiamine + phosphate

717266

-

3.1.3.2

3 entries

Product in Enzyme-catalyzed Reactions (42 results)

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Show Product (42 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole = thiamine monophosphate + diphosphate

639724, 639727, 639722, 639723, 639721, 639719, 639720, 639725, 639726, -

1G4S, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole = thiamine monophosphate + diphosphate

639724, 639727, 639722, 639723, 639721, 639719, 639720, 639725, 639726, -

2TPS, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole = thiamine monophosphate + diphosphate

639724, 639727, 639722, 639723, 639721, 639719, 639720, 639725, 639726, -

3NL3, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole = thiamine monophosphate + diphosphate

639724, 639727, 639722, 639723, 639721, 639719, 639720, 639725, 639726, -

3NL6, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole = thiamine monophosphate + diphosphate

639724, 639727, 639722, 639723, 639721, 639719, 639720, 639725, 639726, -

3NM3, 3D-view

2.5.1.3

4-amino-5-hydroxymethyl-2-methylpyrimidine diphosphate + 4-methyl-5-(2-hydroxyethyl)thiazole phosphate = thiamine monophosphate + diphosphate + phosphate

-

1G4S, 3D-view

2.5.1.3

4-amino-5-hydroxymethyl-2-methylpyrimidine diphosphate + 4-methyl-5-(2-hydroxyethyl)thiazole phosphate = thiamine monophosphate + diphosphate + phosphate

-

2TPS, 3D-view

2.5.1.3

4-amino-5-hydroxymethyl-2-methylpyrimidine diphosphate + 4-methyl-5-(2-hydroxyethyl)thiazole phosphate = thiamine monophosphate + diphosphate + phosphate

-

3NL3, 3D-view

2.5.1.3

4-amino-5-hydroxymethyl-2-methylpyrimidine diphosphate + 4-methyl-5-(2-hydroxyethyl)thiazole phosphate = thiamine monophosphate + diphosphate + phosphate

-

3NL6, 3D-view

2.5.1.3

4-amino-5-hydroxymethyl-2-methylpyrimidine diphosphate + 4-methyl-5-(2-hydroxyethyl)thiazole phosphate = thiamine monophosphate + diphosphate + phosphate

-

3NM3, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + (R,Z)-2-(2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate = diphosphate + thiamine phosphate + CO2

-

1G4S, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + (R,Z)-2-(2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate = diphosphate + thiamine phosphate + CO2

-

2TPS, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + (R,Z)-2-(2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate = diphosphate + thiamine phosphate + CO2

-

3NL3, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + (R,Z)-2-(2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate = diphosphate + thiamine phosphate + CO2

-

3NL6, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + (R,Z)-2-(2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate = diphosphate + thiamine phosphate + CO2

-

3NM3, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate = diphosphate + thiamine phosphate + CO2

-

1G4S, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate = diphosphate + thiamine phosphate + CO2

-

2TPS, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate = diphosphate + thiamine phosphate + CO2

-

3NL3, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate = diphosphate + thiamine phosphate + CO2

-

3NL6, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate = diphosphate + thiamine phosphate + CO2

-

3NM3, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole = thiamine phosphate + diphosphate

-

1G4S, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole = thiamine phosphate + diphosphate

-

2TPS, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole = thiamine phosphate + diphosphate

-

3NL3, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole = thiamine phosphate + diphosphate

-

3NL6, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole = thiamine phosphate + diphosphate

-

3NM3, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate

-

1G4S, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate

-

2TPS, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate

-

3NL3, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate

-

3NL6, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate

-

3NM3, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-beta-hydroxyethylthiazole phosphate = thiamine phosphate + diphosphate

-

1G4S, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-beta-hydroxyethylthiazole phosphate = thiamine phosphate + diphosphate

-

2TPS, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-beta-hydroxyethylthiazole phosphate = thiamine phosphate + diphosphate

-

3NL3, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-beta-hydroxyethylthiazole phosphate = thiamine phosphate + diphosphate

-

3NL6, 3D-view

2.5.1.3

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-beta-hydroxyethylthiazole phosphate = thiamine phosphate + diphosphate

-

3NM3, 3D-view

2.7.1.89

ATP + thiamine = ADP + thiamine monophosphate

642072, -

-

2.7.1.89

ATP + thiamine = ADP + thiamine phosphate

-

-

2.5.1.3

35 entries

2.7.1.89

2 entries

3.1.3.1

thiamin diphosphate + H2O = thiamin phosphate + phosphate

-

-

3.1.3.100

thiamine + phosphate = thiamine phosphate + H2O

-

-

3.1.3.100

thiamine diphosphate + H2O = thiamine phosphate + phosphate

-

-

3.1.3.100

2 entries

3.6.1.28

thiamine diphosphate + H2O = thiamine monophosphate + phosphate

-

-

3.6.1.6

TPP + H2O = thiamine phosphate + phosphate

210206, 210202, 210204

-

Inhibitor in Enzyme-catalyzed Reactions (7 results)

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Show Inhibitors (7 entries)

EC NUMBER

COMMENTARY

LITERATURE

ENZYME 3D STRUCTURE

2.5.1.3

0.1 mM, 59% inhibition

727098

1G4S, 3D-view

2.5.1.3

0.1 mM, 59% inhibition

727098

2TPS, 3D-view

2.5.1.3

0.1 mM, 59% inhibition

727098

3NL3, 3D-view

2.5.1.3

0.1 mM, 59% inhibition

727098

3NL6, 3D-view

2.5.1.3

0.1 mM, 59% inhibition

727098

3NM3, 3D-view

2.5.1.3

5 entries

2.7.6.2

-

642745, 642746

-

3.6.1.28

0.75 mM, 12% inhibition

210007

-

3D Structure of Enzyme-Ligand-Complex (PDB) (45 results)

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Show 3D Structure of Enzyme-Ligand-Complex (PDB) (45 entries)

EC NUMBER

ENZYME 3D STRUCTURE

2.5.1.3

1G4S, 3D-view

2.5.1.3

2TPS, 3D-view

2.5.1.3

3NL3, 3D-view

2.5.1.3

3NL6, 3D-view

2.5.1.3

3NM3, 3D-view

2.5.1.3

1G4S, 3D-view

2.5.1.3

2TPS, 3D-view

2.5.1.3

3NL3, 3D-view

2.5.1.3

3NL6, 3D-view

2.5.1.3

3NM3, 3D-view

2.5.1.3

1G4S, 3D-view

2.5.1.3

2TPS, 3D-view

2.5.1.3

3NL3, 3D-view

2.5.1.3

3NL6, 3D-view

2.5.1.3

3NM3, 3D-view

2.5.1.3

1G4S, 3D-view

2.5.1.3

2TPS, 3D-view

2.5.1.3

3NL3, 3D-view

2.5.1.3

3NL6, 3D-view

2.5.1.3

3NM3, 3D-view

2.7.1.50

3NL3, 3D-view

2.7.1.50

3NL6, 3D-view

2.7.1.50

3NM3, 3D-view

2.7.1.50

3NL3, 3D-view

2.7.1.50

3NL6, 3D-view

2.7.1.50

3NM3, 3D-view

2.7.1.50

3NL3, 3D-view

2.7.1.50

3NL6, 3D-view

2.7.1.50

3NM3, 3D-view

2.7.1.50

3NL3, 3D-view

2.7.1.50

3NL6, 3D-view

2.7.1.50

3NM3, 3D-view

2.7.4.16

3C9T, 3D-view

2.7.4.16

5DD7, 3D-view

2.7.4.16

8YKU, 3D-view

2.7.4.16

3C9T, 3D-view

2.7.4.16

5DD7, 3D-view

2.7.4.16

8YKU, 3D-view

2.7.4.16

3C9T, 3D-view

2.7.4.16

5DD7, 3D-view

2.7.4.16

8YKU, 3D-view

2.7.4.16

3C9T, 3D-view

2.7.4.16

5DD7, 3D-view

2.7.4.16

8YKU, 3D-view

2.5.1.3

20 entries

2.7.1.50

12 entries

2.7.4.16

12 entries

5.3.1.1

2Y6Z, 3D-view

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (4 results)

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Show Turnover Number (4 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

2.7.4.16

0.006

-

pH 7.0, 100°C, recombinant His-tagged mutant S196A

738905

2.7.4.16

0.063

-

pH 7.0, 100°C, recombinant His-tagged wild-type enzyme

738905

2.7.4.16

0.092

-

pH 7.0, 100°C, recombinant His-tagged mutant R136M

738905

2.7.4.16

3 entries

3.1.3.2

4.85

-

pH 37°C, pH 5.6, 1 mM MgCl2, Vmax: 13.3 micromol/min/mg

715966

K_M Value (12 results)

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Show KM Value (12 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

2.7.4.16

0.000043

-

pH 7.0, 100°C, recombinant His-tagged mutant S196A

738905

2.7.4.16

0.0002

-

pH 7.0, 100°C, recombinant His-tagged wild-type enzyme

738905

2.7.4.16

0.0011

-

pH 7.5, 37°C

642545

2.7.4.16

0.0013

-

pH 7.0, 100°C, recombinant His-tagged mutant R136M

738905

2.7.4.16

0.008

-

pH 8.0, temperature not specified in the publication

761512

3.1.3.100

0.049

-

at pH 5.0, temperature not specified in the publication

737016

3.1.3.100

0.6

-

membrane-bound enzyme form, at pH 9.0, temperature not specified in the publication

737294

3.1.3.100

0.7

-

soluble enzyme form, at pH 6.0, temperature not specified in the publication

737294

3.1.3.2

0.0016

-

thiamine-repressible acid phosphatase, pH 5.0, 37°C

717266

3.1.3.2

0.0063

-

thiamine-repressible acid phosphatase, pH 3.0, 37°C

717266

3.1.3.2

0.15

-

pH 37°C, pH 5.6, 1 mM MgCl2, Vmax: 13.3 micromol/min/mg

715966

3.1.3.2

0.27

-

phosphate-repressible acid phosphatase, pH 4.5, 37°C

717266

2.7.4.16

5 entries

3.1.3.100

3 entries

3.1.3.2

4 entries

K_i Value (1 result)

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Show KI Value (1 entry)

EC NUMBER

KI VALUE [MM]

KI VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

2.7.6.2

0.2

-

-

642745

References & Links

Literature References (40)

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Show Reference (40 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

210007

4335862

The partial purification of thiamine triphosphatase from rat brain

1972

Hashitani, Y.; Cooper, J.R.

J. Biol. Chem.

247

2117-2119

210202

2828050

Type B nucleoside-diphosphatase of rat brain. Purification and properties of an enzyme with high thiamin pyrophosphatase activity

1988

Sano, S.; Matsuda, Y.; Nakagawa, H.

Eur. J. Biochem.

171

231-236

210204

6320828

Thiamine pyrophosphatase and nucleoside diphosphatase in rat brain

1984

Sano, S.; Matsuda, Y.; Miyamoto, S.; Nakagawa, H.

Biochem. Biophys. Res. Commun.

118

292-298

210206

-

Rat brain nucleoside diphosphatase: purification and properties of type L isoenzyme

1994

Yang, W.J.; Kim, K.W.; Lee, B.J.

Arch. Pharm. Res.

17

256-262

636860

10945431

Some molecular and enzymatic properties of a homogeneous preparation of thiaminase I purified from carp liver

2000

Bos, M.; Kozik, A.

J. Protein Chem.

19

75-84

639719

13807175

The biosynthesis of thiamine. 2. Fractionation of enzyme system and identification of thiazole monophosphate and thiamine monophosphate as intermediates

1960

Camiener, G.W.; Brown, G.M.

J. Biol. Chem.

235

2411-2417

639720

8394314

Copurification of hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase of Saccharomyces cerevisiae: characterization of hydroxyethylthiazole kinase as a bifunctional enzyme in the thiamine biosynthetic pathway

1993

Kawasaki, Y.

J. Bacteriol.

175

5153-5158

639721

14463407

The enzymatic synthesis of thiamine monophosphate

1961

Leder, I.G.

J. Biol. Chem.

236

3066-3071

639722

4580841

Purification and properties of thiaminephosphate pyrophosphorylase of Escherichia coli

1973

Kayama, Y.; Kawasaki, T.

Arch. Biochem. Biophys.

158

242-248

639723

374983

Thiamine phosphate pyrophosphorylase

1979

Kawasaki, T.

Methods Enzymol.

62

69-73

639724

11513588

Mechanistic studies on thiamin phosphate synthase: evidence for a dissociative mechanism

2001

Reddick, J.J.; Nicewonger, R.; Begley, T.P.

Biochemistry

40

10095-10102

639725

7982968

Isolation and characterization of the THI6 gene encoding a bifunctional thiamin-phosphate pyrophosphorylase/hydroxyethylthiazole kinase from Saccharomyces cerevisiae

1994

Nosaka, K.; Nishimura, H.; Kawasaki, Y.; Tsujihara, T.; Iwashima, A.

J. Biol. Chem.

269

30510-30516

639726

9700068

A Brassica cDNA clone encoding a bifunctional hydroxymethylpyrimidine kinase/thiamin-phosphate pyrophosphorylase involved in thiamin biosynthesis

1998

Kim, Y.S.; Nosaka, K.; Downs, D.M.; Kwak, J.M.; Park, D.; Chung, I.K.; Nam, H.G.

Plant Mol. Biol.

37

955-966

639727

10350464

Crystal structure of thiamin phosphate synthase from Bacillus subtilis at 1.25 A resolution

1999

Chiu, H.J.; Reddick, J.J.; Begley, T.P.; Ealick, S.E.

Biochemistry

38

6460-6470

642072

4550803

Conversion of thiamine to thiamine monophosphate by cell-free extracts of Escherichia coli

1972

Iwashima, A.; Nishino, H.; Nose, Y.

Biochim. Biophys. Acta

258

333-336

642545

4567662

Biogenesis of cocarboxylase in Escherichia coli. Partial purification and some properties of thiamine monophosphate kinase

1972

Nishino, H.

J. Biochem.

72

1093-1100

642546

9188462

Characterization of thiL, encoding thiamin-monophosphate kinase, in Salmonella typhimurium

1997

Webb, E.; Downs, D.

J. Biol. Chem.

272

15702-15707

642745

173819

Mechanism and regulation of thiamine pyrophosphokinase from parsely leaf

1975

Mitsuda, H.; Takii, Y.; Iwami, K.; Yasumoto, K.

J. Nutr. Sci. Vitaminol.

21

189-198

642746

239105

Purification and properties of thiamine pyrophosphokinase from parsely leaf

1975

Mitsuda, H.; Takii, Y.; Kimikazu, I.; Yasumoto, K.

J. Nutr. Sci. Vitaminol.

21

103-115

661335

14592524

An efficient enzymatic synthesis of thiamin pyrophosphate

2003

Melnick, J.S.; Sprinz, K.I.; Reddick, J.J.; Kinsland, C.; Begley, T.P.

Bioorg. Med. Chem. Lett.

13

4139-4141

690944

18311927

Structural studies of thiamin monophosphate kinase in complex with substrates and products

2008

McCulloch, K.M.; Kinsland, C.; Begley, T.P.; Ealick, S.E.

Biochemistry

47

3810-3821

715966

21042033

Determination of Francisella tularensis AcpB acid phosphatase substrate preferences

2010

Crowe, E.; Valladares, R.; Vu, C.; Kuznetsova, E.; Gonzalez, C.F.

J. Mol. Microbiol. Biotechnol.

19

198-203

717266

2407294

High affinity of acid phosphatase encoded by PHO3 gene in Saccharomyces cerevisiae for thiamin phosphates

1990

Nosaka, K.

Biochim. Biophys. Acta

1037

147-154

727098

24583237

Enzymatic and structural characterization of an archaeal thiamin phosphate synthase

2014

Hayashi, M.; Kobayashi, K.; Esaki, H.; Konno, H.; Akaji, K.; Tazuya, K.; Yamada, K.; Nakabayashi, T.; Nosaka, K.

Biochim. Biophys. Acta

1844

803-809

735723

3048416

Identity of soluble thiamin-binding protein with thiamin-repressible acid phosphatase in Saccharomyces cerevisiae

1988

Nosaka, K.; Nishimura, H.; Iwashima, A.

Biochim. Biophys. Acta

967

49-55

735988

2249257

The structural gene coding for thiamin-repressible acid phosphatase in Schizosaccharomyces pombe

1990

Yang, J.; Schweingruber, M.

Curr. Genet.

18

269-272

735989

1868574

Thiamine in Schizosaccharomyces pombe: dephosphorylation, intracellular pool, biosynthesis and transport

1991

Schweingruber, A.; Dlugonski, J.; Edenharter, E.; Schweingruber, M.

Curr. Genet.

19

249-254

737016

19167902

Enzymes that control the thiamine diphosphate pool in plant tissues. Properties of thiamine pyrophosphokinase and thiamine-(di)phosphate phosphatase purified from Zea mays seedlings

2009

Rapala-Kozik, M.; Golda, A.; Kujda, M.

Plant Physiol. Biochem.

47

237-242

737294

25816604

Identification of thiamine monophosphate hydrolyzing enzymes in chicken liver

2014

Kolos, I.; Makarchikov, A.

Ukr. Biochem. J.

86

39-49

738905

26639844

Characterization of thiamin phosphate kinase in the hyperthermophilic archaeon Pyrobaculum calidifontis

2015

Hayashi, M.; Nosaka, K.

J. Nutr. Sci. Vitaminol.

61

369-374

750683

29748198

Dynamic changes in yeast phosphatase families allow for specialization in phosphate and thiamine starvation

2018

Nahas, J.V.; Iosue, C.L.; Shaik, N.F.; Selhorst, K.; He, B.Z.; Wykoff, D.D.

G3 (Bethesda)

8

2333-2343

750683

29748198

Dynamic changes in yeast phosphatase families allow for specialization in phosphate and thiamine starvation

2018

Nahas, J.V.; Iosue, C.L.; Shaik, N.F.; Selhorst, K.; He, B.Z.; Wykoff, D.D.

G3 (Bethesda)

8

2333-2343

751961

28060825

Enhanced activities of blood thiamine diphosphatase and monophosphatase in Alzheimer's disease

2017

Pan, X.; Sang, S.; Fei, G.; Jin, L.; Liu, H.; Wang, Z.; Wang, H.; Zhong, C.

PLoS ONE

12

e0167273

751961

28060825

Enhanced activities of blood thiamine diphosphatase and monophosphatase in Alzheimer's disease

2017

Pan, X.; Sang, S.; Fei, G.; Jin, L.; Liu, H.; Wang, Z.; Wang, H.; Zhong, C.

PLoS ONE

12

e0167273

750683

2 entries

751961

2 entries

752316

-

Copurification of chicken liver soluble thiamine monophosphatase and low molecular weight acid phosphatase

2017

Kolas, I.; Makarchikov, A.

Ukr. Biochem. J.

89

13-21

761512

32404369

The ThiL enzyme is a valid antibacterial target essential for both thiamine biosynthesis and salvage pathways in Pseudomonas aeruginosa

2020

Kim, H.J.; Lee, H.; Lee, Y.; Choi, I.; Ko, Y.; Lee, S.; Jang, S.

J. Biol. Chem.

295

10081-10091

761512

32404369

The ThiL enzyme is a valid antibacterial target essential for both thiamine biosynthesis and salvage pathways in Pseudomonas aeruginosa

2020

Kim, H.J.; Lee, H.; Lee, Y.; Choi, I.; Ko, Y.; Lee, S.; Jang, S.

J. Biol. Chem.

295

10081-10091

762462

30867460

Crystal structures of thiamine monophosphate kinase from Acinetobacter baumannii in complex with substrates and products

2019

Sullivan, A.H.; Dranow, D.M.; Horanyi, P.S.; Lorimer, D.D.; Edwards, T.E.; Abendroth, J.

Sci. Rep.

9

4392

762462

30867460

Crystal structures of thiamine monophosphate kinase from Acinetobacter baumannii in complex with substrates and products

2019

Sullivan, A.H.; Dranow, D.M.; Horanyi, P.S.; Lorimer, D.D.; Edwards, T.E.; Abendroth, J.

Sci. Rep.

9

4392

761512

2 entries

762462

2 entries

775351

34077475

Biochemical characterization of a recombinant acid phosphatase from Acinetobacter baumannii

2021

Smiley-Moreno, E.; Smith, D.; Yu, J.J.; Cao, P.; Arulanandam, B.P.; Chambers, J.P.

PLoS ONE

16

e0252377

Links to other databases for thiamine phosphate

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