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Literature summary for 3.1.3.100 extracted from
- Identity of soluble thiamin-binding protein with thiamin-repressible acid phosphatase in Saccharomyces cerevisiae (1988), Biochim. Biophys. Acta, 967, 49-55.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| thiamine | the enzyme is repressed completely by excess thiamine (0.01 mM) | Saccharomyces cerevisiae |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Saccharomyces cerevisiae | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
x * 56000, SDS-PAGE | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thiamine phosphate + H2O | Saccharomyces cerevisiae | - |
thiamine + phosphate | - |
? | |
| thiamine phosphate + H2O | Saccharomyces cerevisiae X2180-1A | - |
thiamine + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
| Saccharomyces cerevisiae X2180-1A | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Saccharomyces cerevisiae |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-cellulose column chromatography | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thiamine phosphate + H2O | - |
Saccharomyces cerevisiae | thiamine + phosphate | - |
? | |
| thiamine phosphate + H2O | - |
Saccharomyces cerevisiae X2180-1A | thiamine + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 56000, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| thiamin-binding protein | - |
Saccharomyces cerevisiae |
| thiamine-repressible acid phosphatase | - |
Saccharomyces cerevisiae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
the enzyme is inactivated after 10 min at 55°C | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
- |
Saccharomyces cerevisiae |
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