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Information on EC 3.1.1.92 - 4-sulfomuconolactone hydrolase and Organism(s) Hydrogenophaga intermedia and UniProt Accession A6XIG6

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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.92 4-sulfomuconolactone hydrolase
IUBMB Comments
The enzyme was isolated from the bacteria Hydrogenophaga intermedia and Agrobacterium radiobacter S2. It catalyses a step in the degradation of 4-sulfocatechol.
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This record set is specific for:
Hydrogenophaga intermedia
UNIPROT: A6XIG6
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The taxonomic range for the selected organisms is: Hydrogenophaga intermedia
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
SYSTEMATIC NAME
IUBMB Comments
4-sulfomuconolactone sulfohydrolase
The enzyme was isolated from the bacteria Hydrogenophaga intermedia and Agrobacterium radiobacter S2. It catalyses a step in the degradation of 4-sulfocatechol.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-sulfomuconolactone + H2O
maleylacetate + sulfite
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zn2+
0.6 mol of Zn2+ per mol of enzyme
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.9
4-sulfomuconolactone
pH 8.0, temperature not specified in the publication
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
45
4-sulfomuconolactone
pH 8.0, temperature not specified in the publication
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
23
4-sulfomuconolactone
pH 8.0, temperature not specified in the publication
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
79
pH 8.0, temperature not specified in the publication
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
SLMH_HYDIT
303
0
34092
Swiss-Prot
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Halak, S.; Basta, T.; Brger, S.; Contzen, M.; Wray, V.; Pieper, D.H.;, Stolz, A.
4-sulfomuconolactone hydrolases from Hydrogenophaga intermedia S1 and Agrobacterium radiobacter S2
J. Bacteriol.
189
6998-7006
2007
Agrobacterium tumefaciens (A6XIG7), Agrobacterium tumefaciens DSM Z 5681 (A6XIG7), Hydrogenophaga intermedia (A6XIG6), Hydrogenophaga intermedia DSM Z 5680 (A6XIG6)
Manually annotated by BRENDA team