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Literature summary for 3.1.1.92 extracted from

  • Halak, S.; Basta, T.; Bürger, S.; Contzen, M.; Wray, V.; Pieper, D.H.;, Stolz, A.
    4-sulfomuconolactone hydrolases from Hydrogenophaga intermedia S1 and Agrobacterium radiobacter S2 (2007), J. Bacteriol., 189, 6998-7006.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.92

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Hydrogenophaga intermedia
expressed in Escherichia coli Agrobacterium tumefaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.34
-
 4-sulfomuconolactone pH 8.0, temperature not specified in the publication Agrobacterium tumefaciens
1.9
-
 4-sulfomuconolactone pH 8.0, temperature not specified in the publication Hydrogenophaga intermedia

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ 0.6 mol of Zn2+ per mol of enzyme Hydrogenophaga intermedia

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens A6XIG7
-
-
Agrobacterium tumefaciens DSM Z 5681 A6XIG7
-
-
Hydrogenophaga intermedia A6XIG6
-
-
Hydrogenophaga intermedia DSM Z 5680 A6XIG6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Hydrogenophaga intermedia
-
 Agrobacterium tumefaciens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.6
-
 pH 8.0, temperature not specified in the publication Agrobacterium tumefaciens
79
-
 pH 8.0, temperature not specified in the publication Hydrogenophaga intermedia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-sulfomuconolactone + H2O
-
 Hydrogenophaga intermedia maleylacetate + sulfite
-
 ?
4-sulfomuconolactone + H2O
-
 Agrobacterium tumefaciens maleylacetate + sulfite
-
 ?
4-sulfomuconolactone + H2O
-
 Hydrogenophaga intermedia DSM Z 5680 maleylacetate + sulfite
-
 ?
4-sulfomuconolactone + H2O
-
 Agrobacterium tumefaciens DSM Z 5681 maleylacetate + sulfite
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.7
-
 4-sulfomuconolactone pH 8.0, temperature not specified in the publication Agrobacterium tumefaciens
45
-
 4-sulfomuconolactone pH 8.0, temperature not specified in the publication Hydrogenophaga intermedia

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
20
-
 4-sulfomuconolactone pH 8.0, temperature not specified in the publication Agrobacterium tumefaciens
23
-
 4-sulfomuconolactone pH 8.0, temperature not specified in the publication Hydrogenophaga intermedia