This plant enzyme is responsible for the last stages in the biosynthesis of the indole alkaloid ajmaline. The enzyme is highly specific for the substrates 17-O-acetylajmaline and 17-O-acetylnorajmaline as the structurally related acetylated alkaloids vinorine, vomilenine, 1,2-dihydrovomilenine and 1,2-dihydroraucaffricine cannot act as substrates . This is a novel member of the GDSL family of serine esterases/lipases.
The expected taxonomic range for this enzyme is: Rauvolfia
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SYSTEMATIC NAME
IUBMB Comments
17-O-acetylajmaline O-acetylhydrolase
This plant enzyme is responsible for the last stages in the biosynthesis of the indole alkaloid ajmaline. The enzyme is highly specific for the substrates 17-O-acetylajmaline and 17-O-acetylnorajmaline as the structurally related acetylated alkaloids vinorine, vomilenine, 1,2-dihydrovomilenine and 1,2-dihydroraucaffricine cannot act as substrates [2]. This is a novel member of the GDSL family of serine esterases/lipases.
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
the expression in Escherichia coli failed although a wide range of conditions were tested. With a virus-based plant expression system, it was possible to express the enzyme functionally in leaves of Nicotiana benthamiana Domin. An extraordinarily high enzyme activity is detected in the Nicotiana tissue, which exceeds that in Rauvolfia cell suspension cultures about 20fold
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