General Stability | Organism |
---|---|
4°C, at the stage of a 150fold enrichment in 0.1 M phosphate buffer, pH 7.0, 1 mM dithiothreitol, half-life: 21 days | Rauvolfia serpentina |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-dithiodipyridine | 0.5 mM, 30-40% inhibition | Rauvolfia serpentina | |
5,5'-dithiobis(2-nitrobenzoic acid) | 0.5 mM, 30-40% inhibition | Rauvolfia serpentina | |
iodoacetamide | 0.5 mM, 30-40% inhibition | Rauvolfia serpentina |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
gel filtration | Rauvolfia serpentina |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rauvolfia serpentina | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Rauvolfia serpentina |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Rauvolfia serpentina | - |
leaf | - |
Rauvolfia serpentina | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0364 | - |
- |
Rauvolfia serpentina |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
17-O-acetylajmaline + H2O | 84.2% of the activity with 17-O-acetylnorajmaline | Rauvolfia serpentina | ajmaline + acetate | - |
? | |
17-O-acetylnorajmaline + H2O | - |
Rauvolfia serpentina | norajmaline + acetate | - |
? | |
17-O-acetyltetraphyllicine + H2O | 86% of the activity with 17-O-acetylnorajmaline | Rauvolfia serpentina | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Rauvolfia serpentina |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Rauvolfia serpentina |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.3 | 8.5 | pH 6.3: about 50% of maximal activity, pH 8.5: about 50% of maximal activity | Rauvolfia serpentina |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rauvolfia serpentina | isoelectric focusing | - |
4.9 |