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Information on EC 3.1.1.77 - acyloxyacyl hydrolase and Organism(s) Homo sapiens and UniProt Accession P28039
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3.1.1.77 acyloxyacyl hydrolaseSpecify your search results
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The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
acyloxyacyl hydrolase, neutrophil acyloxyacyl hydrolase, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
neutrophil acyloxyacyl hydrolase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of C-O bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
110277-64-0
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
3-(acyloxy)acyl group of bacterial toxin
3-hydroxyacyl group of bacterial toxin + a fatty acid
3-(acyloxy)acyl group of bacterial toxin + H2O
3-hydroxyacyl group of bacterial toxin + a fatty acid
sn-1,2-dipalmitoylphosphatidylcholine + H2O
palmitate + phosphatidylcholine
-
in vitro
-
?
3-(acyloxy)acyl group of bacterial toxin
3-hydroxyacyl group of bacterial toxin + a fatty acid
-
detoxification of LPS
-
?
3-(acyloxy)acyl group of bacterial toxin
3-hydroxyacyl group of bacterial toxin + a fatty acid
-
detoxification of LPS
-
?
3-(acyloxy)acyl group of bacterial toxin
3-hydroxyacyl group of bacterial toxin + a fatty acid
-
detoxification of LPS
-
?
3-(acyloxy)acyl group of bacterial toxin
3-hydroxyacyl group of bacterial toxin + a fatty acid
-
detoxification of LPS
-
?
3-(acyloxy)acyl group of bacterial toxin
3-hydroxyacyl group of bacterial toxin + a fatty acid
-
detoxification of LPS
-
?
3-(acyloxy)acyl group of bacterial toxin
3-hydroxyacyl group of bacterial toxin + a fatty acid
-
detoxification of LPS
-
?
3-(acyloxy)acyl group of bacterial toxin
3-hydroxyacyl group of bacterial toxin + a fatty acid
-
detoxification of LPS
-
?
3-(acyloxy)acyl group of bacterial toxin
3-hydroxyacyl group of bacterial toxin + a fatty acid
-
detoxification of LPS
-
?
3-(acyloxy)acyl group of bacterial toxin
3-hydroxyacyl group of bacterial toxin + a fatty acid
-
detoxification of LPS
-
?
3-(acyloxy)acyl group of bacterial toxin + H2O
3-hydroxyacyl group of bacterial toxin + a fatty acid
-
-
-
?
3-(acyloxy)acyl group of bacterial toxin + H2O
3-hydroxyacyl group of bacterial toxin + a fatty acid
-
-
-
?
3-(acyloxy)acyl group of bacterial toxin + H2O
3-hydroxyacyl group of bacterial toxin + a fatty acid
-
-
-
?
3-(acyloxy)acyl group of bacterial toxin + H2O
3-hydroxyacyl group of bacterial toxin + a fatty acid
-
-
-
?
3-(acyloxy)acyl group of bacterial toxin + H2O
3-hydroxyacyl group of bacterial toxin + a fatty acid
-
-
-
?
3-(acyloxy)acyl group of bacterial toxin + H2O
3-hydroxyacyl group of bacterial toxin + a fatty acid
-
-
-
?
3-(acyloxy)acyl group of bacterial toxin + H2O
3-hydroxyacyl group of bacterial toxin + a fatty acid
-
-
-
?
3-(acyloxy)acyl group of bacterial toxin + H2O
3-hydroxyacyl group of bacterial toxin + a fatty acid
-
-
-
?
3-(acyloxy)acyl group of bacterial toxin + H2O
3-hydroxyacyl group of bacterial toxin + a fatty acid
-
smooth LPS deacylated more slowly than rough LPS
palmitoleate released more slowly than myristate and laurate, 2-hydroxylaurate released more slowly than laurate
?
additional information
?
-
polymorphisms in markers within the acyloxyacyl hydroxylase gene are associated with the risk of asthma and associated quantitative traits (IgE and cytokine levels) among asthmatic subjects and their families in Barbados
-
-
?
additional information
?
-
-
endotoxin-binding proteins modulate the susceptibility of bacterial endotoxin to deacylation by acyloxyacyl hydrolase
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
3-(acyloxy)acyl group of bacterial toxin
3-hydroxyacyl group of bacterial toxin + a fatty acid
3-(acyloxy)acyl group of bacterial toxin
3-hydroxyacyl group of bacterial toxin + a fatty acid
-
detoxification of LPS
-
?
3-(acyloxy)acyl group of bacterial toxin
3-hydroxyacyl group of bacterial toxin + a fatty acid
-
detoxification of LPS
-
?
3-(acyloxy)acyl group of bacterial toxin
3-hydroxyacyl group of bacterial toxin + a fatty acid
-
detoxification of LPS
-
?
3-(acyloxy)acyl group of bacterial toxin
3-hydroxyacyl group of bacterial toxin + a fatty acid
-
detoxification of LPS
-
?
3-(acyloxy)acyl group of bacterial toxin
3-hydroxyacyl group of bacterial toxin + a fatty acid
-
detoxification of LPS
-
?
3-(acyloxy)acyl group of bacterial toxin
3-hydroxyacyl group of bacterial toxin + a fatty acid
-
detoxification of LPS
-
?
3-(acyloxy)acyl group of bacterial toxin
3-hydroxyacyl group of bacterial toxin + a fatty acid
-
detoxification of LPS
-
?
3-(acyloxy)acyl group of bacterial toxin
3-hydroxyacyl group of bacterial toxin + a fatty acid
-
detoxification of LPS
-
?
3-(acyloxy)acyl group of bacterial toxin
3-hydroxyacyl group of bacterial toxin + a fatty acid
-
detoxification of LPS
-
?
additional information
?
-
polymorphisms in markers within the acyloxyacyl hydroxylase gene are associated with the risk of asthma and associated quantitative traits (IgE and cytokine levels) among asthmatic subjects and their families in Barbados
-
-
?
additional information
?
-
-
endotoxin-binding proteins modulate the susceptibility of bacterial endotoxin to deacylation by acyloxyacyl hydrolase
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
Ca2+
-
5 mM enhances activity by 30-50% at low substrate concentration, inhibitory at high substrate concentration
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
-
acyloxyacyl hydrolase acts as one of the diverse antimicrobial peptides and proteins expressed in the placenta
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). AOAH_HUMAN
575
0
65105
Swiss-Prot
Secretory Pathway (Reliability: 1)
PDB
SCOP
CATH
UNIPROT
ORGANISM
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
14000 - 20000
-
small subunit, SDS-PAGE in presence of 2-mercaptoethanol
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dimer
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
glycoprotein
glycoprotein
-
five potential glycosylation sites, one on the small and four on the large subunit
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
slight downregulation of enzyme expression by treatment during 24 h lipopolysaccharide or UV-treated group B streptococci, overview
-
the enzyme is induced by UV-treated Propionibacterium acnes treatment after 24 h
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Erwin, A.L.; Munford, R.S.
Deacylation of structurally diverse lipopolysaccharides by human acyloxyacyl hydrolase
J. Biol. Chem.
265
16444-16449
1990
Homo sapiens
Hagen, F.S.; Grant, F.J.; Kuijper, J.L.; Slaughter, C.A.; Moomaw, C.R.; Orth, K.; O'Hara, P.J.; Munford, R.S.
Expression and characterization of recombinant human acyloxyacyl hydrolase, a leukocyte enzyme that deacylates bacterial lipopolysaccharides
Biochemistry
30
8415-8423
1991
Homo sapiens
Munford, R.S.; Hunter, J.P.
Acyloxyacyl hydrolase, a leukocyte enzyme that deacylates bacterial lipopolysaccharides, has phospholipase, lysophospholipase, diacylglycerollipase, and acyltransferase activities in vitro
J. Biol. Chem.
267
10116-10121
1992
Homo sapiens
Munford, R.S.; Hall, C.L.
Detoxification of bacterial lipopolysaccharides (endotoxins) by a human neutrophil enzyme
Science
234
203-205
1986
Homo sapiens
Munford, R.S.; Hall, C.L.
Purification of acyloxyacyl hydrolase, a leukocyte enzyme that removes secondary acyl chains from bacterial lipopolysaccharides
J. Biol. Chem.
264
15613-15619
1989
Homo sapiens
Mc Dermott, C.M.; Cullor, J.S.; Fenwick, B.W.
Intracellular and extracellular enzymatic deacylation of bacterial endotoxin during localized inflammation induced by Escherichia coli
Infect. Immun.
59
487-485
1991
Bos taurus, Homo sapiens
-
Munford, R.S.; Erwin, A.L.
Eukaryotic lipopolysaccharide deacylation enzyme
Methods Enzymol.
209
485-492
1992
Homo sapiens
Staab, J.F.; Ginkel, D.L.; Rosenberg, G.B.; Munford, R.S.
A saposin-like domain influences the intracellular localization, stability, and catalytic activity of human acyloxyacyl hydrolase
J. Biol. Chem.
269
23736-23742
1994
Homo sapiens
Staab, J.F.; Fosmire, S.; Zhang, M.; Varley, A.W.; Munford, R.S.
Distinctive structural features are shared by human, lapine, and murine acloxyacyl hydrolases
J. Endotoxin Res.
5
205-208
1999
Oryctolagus cuniculus, Homo sapiens, Mus musculus
-
Barnes, K.C.; Grant, A.; Gao, P.; Baltadjieva, D.; Berg, T.; Chi, P.; Zhang, S.; Zambelli-Weiner, A.; Ehrlich, E.; Zardkoohi, O.; Brummet, M.E.; Stockton, M.; Watkins, T.; Gao, L.; Gittens, M.; Wills-Karp, M.; Cheadle, C.; Beck, L.A.; Beaty, T.H.; Becker, K.G.; garcia, J.G.N.; Mathias, R.A.
Polymorphisms in the novel gene acyloxyacyl hydroxylase (AOAH) are associated with asthma and associated phenotypes
J. Allergy Clin. Immunol.
118
70-77
2006
Homo sapiens (P28039)
Gioannini, T.L.; Teghanemt, A.; Zhang, D.; Prohinar, P.; Levis, E.N.; Munford, R.S.; Weiss, J.P.
Endotoxin-binding proteins modulate the susceptibility of bacterial endotoxin to deacylation by acyloxyacyl hydrolase
J. Biol. Chem.
282
7877-7884
2007
Homo sapiens
Klaffenbach, D.; Friedrich, D.; Strick, R.; Strissel, P.L.; Beckmann, M.W.; Rascher, W.; Gessner, A.; Doetsch, J.; Meissner, U.; Schnare, M.
Contribution of different placental cells to the expression and stimulation of antimicrobial proteins (AMPs)
Placenta
32
830-837
2011
Homo sapiens
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