BRENDA - Enzyme Database
show all sequences of 3.1.1.77

Purification of acyloxyacyl hydrolase, a leukocyte enzyme that removes secondary acyl chains from bacterial lipopolysaccharides

Munford, R.S.; Hall, C.L.; J. Biol. Chem. 264, 15613-15619 (1989)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
dimethylsulfate
induces enzyme activity when added to the medium
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
complete inhibition at 0.1 M
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00055
-
lipopolysaccharide
-
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Homo sapiens
16020
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
14000
20000
small subunit, SDS-PAGE in presence of 2-mercaptoethanol
Homo sapiens
48000
-
SDS-PAGE of large subunit after N-glycanase treatment
Homo sapiens
50000
-
large subunit, SDS-PAGE in presence of 2-mercaptoethanol
Homo sapiens
52000
60000
native PAGE
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-(acyloxy)acyl group of bacterial toxin
Homo sapiens
detoxification of LPS
3-hydroxyacyl group of bacterial toxin + a fatty acid
-
Homo sapiens
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
glycoprotein
both subunits glycosylated
Homo sapiens
Purification (Commentary)
Commentary
Organism
-
Homo sapiens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
HL-60 cell
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.000005
-
cell lysate
Homo sapiens
1.555
-
purified enzyme
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-(acyloxy)acyl group of bacterial toxin
detoxification of LPS
246549
Homo sapiens
3-hydroxyacyl group of bacterial toxin + a fatty acid
-
246549
Homo sapiens
-
3-(acyloxy)acyl group of bacterial toxin + H2O
-
246549
Homo sapiens
3-hydroxyacyl group of bacterial toxin + a fatty acid
-
246549
Homo sapiens
?
Subunits
Subunits
Commentary
Organism
dimer
alpha, beta, 1 * 50000 + 1 * 14000-20000, disulfide linked
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.8
5
-
Homo sapiens
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
dimethylsulfate
induces enzyme activity when added to the medium
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
complete inhibition at 0.1 M
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00055
-
lipopolysaccharide
-
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Homo sapiens
16020
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
14000
20000
small subunit, SDS-PAGE in presence of 2-mercaptoethanol
Homo sapiens
48000
-
SDS-PAGE of large subunit after N-glycanase treatment
Homo sapiens
50000
-
large subunit, SDS-PAGE in presence of 2-mercaptoethanol
Homo sapiens
52000
60000
native PAGE
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-(acyloxy)acyl group of bacterial toxin
Homo sapiens
detoxification of LPS
3-hydroxyacyl group of bacterial toxin + a fatty acid
-
Homo sapiens
-
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
glycoprotein
both subunits glycosylated
Homo sapiens
Purification (Commentary) (protein specific)
Commentary
Organism
-
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
HL-60 cell
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.000005
-
cell lysate
Homo sapiens
1.555
-
purified enzyme
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-(acyloxy)acyl group of bacterial toxin
detoxification of LPS
246549
Homo sapiens
3-hydroxyacyl group of bacterial toxin + a fatty acid
-
246549
Homo sapiens
-
3-(acyloxy)acyl group of bacterial toxin + H2O
-
246549
Homo sapiens
3-hydroxyacyl group of bacterial toxin + a fatty acid
-
246549
Homo sapiens
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
alpha, beta, 1 * 50000 + 1 * 14000-20000, disulfide linked
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.8
5
-
Homo sapiens
Other publictions for EC 3.1.1.77
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
749541
Yang
Acyloxyacyl hydrolase modulat ...
Mus musculus
Am. J. Physiol. Regul. Integr. Comp. Physiol.
314
R353-R365
2018
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1
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1
1
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751999
Zou
Acyloxyacyl hydrolase promote ...
Mus musculus
PLoS Pathog.
13
e1006436
2017
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1
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4
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1
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1
1
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730832
Janelsins
Altered inactivation of commen ...
Mus musculus
Proc. Natl. Acad. Sci. USA
111
373-378
2014
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-
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1
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3
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5
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1
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5
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1
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2
2
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715145
Shao
Prolonged hepatomegaly in mice ...
Mus musculus
Hepatology
54
1051-1062
2011
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-
-
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6
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1
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1
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2
2
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-
716472
Klaffenbach
Contribution of different plac ...
Homo sapiens
Placenta
32
830-837
2011
-
-
-
-
-
-
-
-
-
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2
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3
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3
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-
-
-
-
-
-
-
-
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2
1
1
2
-
-
704964
Ojogun
Overproduction of acyloxyacyl ...
Mus musculus
J. Infect. Dis.
200
1685-1693
2009
-
1
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-
-
-
-
-
-
-
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5
-
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1
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1
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1
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1
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1
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1
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1
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1
1
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-
691683
Lu
Host inactivation of bacterial ...
Mus musculus
Cell host microbe
4
293-302
2008
-
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1
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-
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-
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4
-
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1
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680766
Shao
A host lipase detoxifies bacte ...
Mus musculus
J. Biol. Chem.
282
13726-13735
2007
-
-
-
-
-
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1
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3
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1
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1
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1
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1
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1
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680889
Gioannini
Endotoxin-binding proteins mod ...
Homo sapiens
J. Biol. Chem.
282
7877-7884
2007
-
-
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1
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2
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1
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1
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682968
Mehrzad
Bovine blood neutrophil acylox ...
Bos taurus
Vet. Res.
38
655-668
2007
-
-
-
-
-
-
-
-
-
-
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1
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3
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3
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1
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1
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3
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1
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680332
Barnes
Polymorphisms in the novel gen ...
Homo sapiens
J. Allergy Clin. Immunol.
118
70-77
2006
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1
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1
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665166
feulner
Identification of acyloxyacyl ...
Mus musculus
Infect. Immun.
72
3171-3178
2004
-
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1
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3
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1
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7
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2
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1
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1
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7
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2
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665875
Lu
Stimulus-dependent deacylation ...
Mus musculus
J. EXp. Med.
197
1745-1754
2003
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3
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1
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246554
Staab
-
Distinctive structural feature ...
Homo sapiens, Mus musculus, Oryctolagus cuniculus
J. Endotoxin Res.
5
205-208
1999
-
-
3
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3
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3
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3
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3
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6
3
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3
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3
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3
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3
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6
3
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246553
Cody
-
Effect of inflammatory and ant ...
Mus musculus
J. Endotoxin Res.
4
371-379
1997
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246552
Staab
A saposin-like domain influenc ...
Homo sapiens
J. Biol. Chem.
269
23736-23742
1994
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1
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1
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1
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1
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1
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246547
Munford
Acyloxyacyl hydrolase, a leuko ...
Homo sapiens
J. Biol. Chem.
267
10116-10121
1992
2
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3
2
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1
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2
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1
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2
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8
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1
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2
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3
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2
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1
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1
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2
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8
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1
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246551
Munford
Eukaryotic lipopolysaccharide ...
Homo sapiens
Methods Enzymol.
209
485-492
1992
1
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1
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1
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1
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2
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1
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1
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2
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2
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246546
Hagen
Expression and characterizatio ...
Homo sapiens
Biochemistry
30
8415-8423
1991
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1
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4
1
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2
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1
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2
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2
1
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1
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4
1
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1
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2
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2
1
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246550
Mc Dermott
-
Intracellular and extracellula ...
Bos taurus, Homo sapiens
Infect. Immun.
59
487-485
1991
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2
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2
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2
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4
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2
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2
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4
-
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1
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2
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246545
Erwin
Deacylation of structurally di ...
Homo sapiens
J. Biol. Chem.
265
16444-16449
1990
1
1
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3
-
1
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