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Information on EC - acyloxyacyl hydrolase

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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
       acyloxyacyl hydrolase
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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
acyloxyacyl hydrolase, neutrophil acyloxyacyl hydrolase, more
3-(acyloxy)acyl group of bacterial toxin + H2O = 3-hydroxyacyl group of bacterial toxin + a fatty acid
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The substrate is lipid A on the reducing end of the toxic lipopolysaccharide, LPS of Salmonella typhimurium and related organisms. It consists of diglucosamine, beta-D-GlcN-(1- 6)-D-GlcN, attached by glycosylation on O-6 of its non-reducing residue, phosphorylated on O-4 of this residue and on O-1 of ist potentially reducing residue. Both residues carry 3-(acyloxy)acyl groups on N-2 and O-3. The enzyme from human leucocytes detoxifies the lipid by hydrolysing the secondary acyl groups from O-3 of the 3-hydroxyacyl groups on the disaccharide, LPS. It also possesses a wide range of phospholipase and acyltransferase activities, e.g. EC, phospholipase A2, EC, lysophospholipase, EC, phospholipase A1 and EC, phosphatidylinositol deacylase, hydrolysing diacylglycerol and phosphatidyl compounds, but not triacylglycerols. It has a preference for saturated C12-C16 acyl groups