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EC Tree
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
lactonase, limonin-D-ring, LDLH, LDRLase, limonin lactone hydrolase, limonin-D-ring-lactone hydrolase,
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lactonase, limonin-D-ring
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limonin lactone hydrolase
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limonin-D-ring-lactone hydrolase
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limonoate D-ring-lactone + H2O = limonoate
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hydrolysis of carboxylic ester
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limonoate-D-ring-lactone lactonohydrolase
Limonoate is a triterpenoid.
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limonin
limonoate-A-ring-lactone + H2O
limonoate
limonoate D-ring-lactone + H2O
Citrus nobilis x Citrus deliciosa
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?
limonoate
limonoate-D-ring-lactone + H2O
limonoate-A-ring-lactone + H2O
limonin
limonoate-D-ring-lactone + H2O
limonoate
nomilin
nomilinoate A-ring lactone + H2O
ichangin + H2O
?
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?
ichangin + H2O
?
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?
limonin
limonoate-A-ring-lactone + H2O
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?
limonin
limonoate-A-ring-lactone + H2O
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?
limonin
limonoate-A-ring-lactone + H2O
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r
limonin
limonoate-A-ring-lactone + H2O
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r
limonin
limonoate-A-ring-lactone + H2O
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r
limonin
limonoate-A-ring-lactone + H2O
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r
limonin
limonoate-A-ring-lactone + H2O
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r
limonoate
limonoate-D-ring-lactone + H2O
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r
limonoate
limonoate-D-ring-lactone + H2O
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r
limonoate
limonoate-D-ring-lactone + H2O
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r
limonoate
limonoate-D-ring-lactone + H2O
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r
limonoate-A-ring-lactone + H2O
limonin
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r
limonoate-A-ring-lactone + H2O
limonin
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r
limonoate-A-ring-lactone + H2O
limonin
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?
limonoate-A-ring-lactone + H2O
limonin
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?
limonoate-D-ring-lactone + H2O
limonoate
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?
limonoate-D-ring-lactone + H2O
limonoate
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r
limonoate-D-ring-lactone + H2O
limonoate
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r
limonoate-D-ring-lactone + H2O
limonoate
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r
limonoate-D-ring-lactone + H2O
limonoate
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r
nomilin
nomilinoate A-ring lactone + H2O
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?
nomilin
nomilinoate A-ring lactone + H2O
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?
nomilin
nomilinoate A-ring lactone + H2O
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?
nomilin
nomilinoate A-ring lactone + H2O
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?
obacunone + H2O
?
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?
obacunone + H2O
?
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?
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1,2-cyclohexylenedinitrilotetraacetic acid
Citrus nobilis x Citrus deliciosa
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20 mM, 40% inhibition
Bromelain
Citrus nobilis x Citrus deliciosa
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L-glutamate
Citrus nobilis x Citrus deliciosa
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20 mM, 40% inhibition
sodium hexametaphosphate
Citrus nobilis x Citrus deliciosa
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20 mM, 41% inhibition
additional information
Citrus nobilis x Citrus deliciosa
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not inhibitory: papain, pepsin, histidine and DL-malic acid
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EDTA
Citrus nobilis x Citrus deliciosa
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20 mM, 58% inhibition
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566
Citrus nobilis x Citrus deliciosa
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pH 8.0, 37°C
additional information
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fluorescence assay method for purified and crude enzyme preparations
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6
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6
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lactonization of limonin
6
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lactonization of limonin
8
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hydrolysis of limonoate A-ring lactone
8
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hydrolysis of limonoate A-ring lactone
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brenda
Citrus nobilis x Citrus deliciosa
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brenda
321-18
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brenda
321-18
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brenda
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brenda
grapefruit
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brenda
Valencia orange
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brenda
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Citrus nobilis x Citrus deliciosa
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brenda
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brenda
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224000
Citrus nobilis x Citrus deliciosa
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gel filtration
250000
Citrus nobilis x Citrus deliciosa
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PAGE
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pentamer
Citrus nobilis x Citrus deliciosa
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5 * 45000, SDS-PAGE
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100
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5 min, 70% loss of activity
53
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5 min, 50% loss of activity
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from seed
Citrus nobilis x Citrus deliciosa
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Maier, V.P.; Hasegawa, S.; Hera, E.
Limonin D-ring-lactone hydrolase. A new enzyme from Citrus seeds
Phytochemistry
8
405-407
1969
Citrus sinensis
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brenda
Hasegawa, S.
Metabolism of limonoids. Limonin D-ring lactone hydrolase activity in Pseudomonas
J. Agric. Food Chem.
24
24-26
1976
Citrus sinensis, Pseudomonas sp., Pseudomonas sp. 321-18
brenda
Hasegawa, S.; Patel, M.N.; Snyder, R.C.
Reduction of bitterness in navel orange juicse serum with bacterial cells immobilized in acrylamide gels
J. Agric. Food Chem.
30
509-511
1982
Arthrobacter globiformis
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brenda
Hasegawa, S.; Pelton, V.A.
Reduction of nomilin bitterness in Citrus juices and juice serum with Arthrobacter globiformis cells immobilized in acrylamide gel
J. Agric. Food Chem.
31
178-180
1983
Arthrobacter globiformis
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brenda
Merino, M.T.; Humanes, L.; Lopez-Ruiz, A.; Roldan, J.M.
High-performance liquid chromatography quantitation of limonin D-ring lactone hydrolase and limonoate dehydrogenase activities
J. Chromatogr. A
760
173-178
1997
Citrus sinensis
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brenda
Breksa, A.P.; Manners, G.D.
Determination of limonin D-ring lactone hydrolase activity by solid phase extraction with indirect fluorescence detection
J. Agric. Food Chem.
52
3772-3775
2004
Citrus sinensis
brenda
Kumar, S.; Kumar, R.; Sharma, P.; Pal, A.
Purification and characterization of limonoate-D-ring lactone hydrolase from kinnow fruits grown in Punjab, India
J. Environ. Biol.
39
175-181
2018
Citrus nobilis x Citrus deliciosa
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brenda
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3.1.1.36 limonin-D-ring-lactonase
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