Any feedback?
No. of entries
Information on EC 3.1.1.36 - limonin-D-ring-lactonase
for references in articles please use BRENDA:EC3.1.1.36
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree 3 Hydrolases
3.1 Acting on ester bonds
3.1.1 Carboxylic-ester hydrolases
3.1.1.36 limonin-D-ring-lactonase
IUBMB Comments
Limonoate is a triterpenoid.
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
showSynonyms
lactonase, limonin-D-ring, LDLH, LDRLase, limonin lactone hydrolase, limonin-D-ring-lactone hydrolase, more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
lactonase, limonin-D-ring
-
-
-
-
LDRLase
-
-
-
-
limonin lactone hydrolase
-
-
-
-
limonin-D-ring-lactone hydrolase
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
limonoate D-ring-lactone + H2O = limonoate
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of carboxylic ester
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
limonoate-D-ring-lactone lactonohydrolase
Limonoate is a triterpenoid.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
9031-17-8
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
limonoate
limonoate D-ring-lactone + H2O
Citrus nobilis x Citrus deliciosa
-
Substrates: -
Products: -
Products: -
?
limonin
limonoate-A-ring-lactone + H2O
-
Substrates: -
Products: -
Products: -
r
limonoate-D-ring-lactone + H2O
limonoate
-
Substrates: -
Products: -
Products: -
?
nomilin
nomilinoate A-ring lactone + H2O
-
Substrates: -
Products: -
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1,2-cyclohexylenedinitrilotetraacetic acid
Citrus nobilis x Citrus deliciosa
-
20 mM, 40% inhibition
additional information
Citrus nobilis x Citrus deliciosa
-
not inhibitory: papain, pepsin, histidine and DL-malic acid
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
fluorescence assay method for purified and crude enzyme preparations
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Maier, V.P.; Hasegawa, S.; Hera, E.
Limonin D-ring-lactone hydrolase. A new enzyme from Citrus seeds
Phytochemistry
8
405-407
1969
Citrus sinensis
-
brenda
Hasegawa, S.
Metabolism of limonoids. Limonin D-ring lactone hydrolase activity in Pseudomonas
J. Agric. Food Chem.
24
24-26
1976
Citrus sinensis, Pseudomonas sp., Pseudomonas sp. 321-18
brenda
Hasegawa, S.; Patel, M.N.; Snyder, R.C.
Reduction of bitterness in navel orange juicse serum with bacterial cells immobilized in acrylamide gels
J. Agric. Food Chem.
30
509-511
1982
Arthrobacter globiformis
-
brenda
Hasegawa, S.; Pelton, V.A.
Reduction of nomilin bitterness in Citrus juices and juice serum with Arthrobacter globiformis cells immobilized in acrylamide gel
J. Agric. Food Chem.
31
178-180
1983
Arthrobacter globiformis
-
brenda
Merino, M.T.; Humanes, L.; Lopez-Ruiz, A.; Roldan, J.M.
High-performance liquid chromatography quantitation of limonin D-ring lactone hydrolase and limonoate dehydrogenase activities
J. Chromatogr. A
760
173-178
1997
Citrus sinensis
-
brenda
Breksa, A.P.; Manners, G.D.
Determination of limonin D-ring lactone hydrolase activity by solid phase extraction with indirect fluorescence detection
J. Agric. Food Chem.
52
3772-3775
2004
Citrus sinensis
brenda
Kumar, S.; Kumar, R.; Sharma, P.; Pal, A.
Purification and characterization of limonoate-D-ring lactone hydrolase from kinnow fruits grown in Punjab, India
J. Environ. Biol.
39
175-181
2018
Citrus nobilis x Citrus deliciosa
-
brenda
EXTERNAL LINKS (specific for EC-Number 3.1.1.36)
html completed
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2026.1 (March 2026)
About BRENDA
Social media
Help
Survey
Download
Contribution
Legal
Curated at
Member of
![DSMZ Digital Diversity]()
![Global Core Biodata Resource]()
![ELIXIR Core Data Resource]()
![German Network for Bioinformatics Infrastructure]()
