Information on EC 3.1.1.36 - limonin-D-ring-lactonase

for references in articles please use BRENDA:EC3.1.1.36
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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
3.1.1.36
-
RECOMMENDED NAME
GeneOntology No.
limonin-D-ring-lactonase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
limonoate D-ring-lactone + H2O = limonoate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of carboxylic ester
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
limonoate-D-ring-lactone lactonohydrolase
Limonoate is a triterpenoid.
CAS REGISTRY NUMBER
COMMENTARY hide
9031-17-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
321-18
-
-
Manually annotated by BRENDA team
321-18
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ichangin + H2O
?
show the reaction diagram
limonin + H2O
limonoate-A-ring-lactone
show the reaction diagram
limonoate
limonoate-D-ring-lactone + H2O
show the reaction diagram
limonoate-A-ring-lactone
limonin + H2O
show the reaction diagram
limonoate-D-ring-lactone + H2O
limonoate
show the reaction diagram
nomilin + H2O
nomilinoate A-ring lactone
show the reaction diagram
obacunone + H2O
?
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.6
-
pH 8.0, 30°C
additional information
-
fluorescence assay method for purified and crude enzyme preparations
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
53
-
5 min, 50% loss of activity
100
-
5 min, 70% loss of activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
lyophilized
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE