This enzyme, characterized from cruciferous plants, catalyses the last step in the biosynthesis of tryptophan- and phenylalanine-derived glucosinolates. cf. EC 2.8.2.38, aliphatic desulfoglucosinolate sulfotransferase.
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The taxonomic range for the selected organisms is: Arabidopsis thaliana The enzyme appears in selected viruses and cellular organisms
This enzyme, characterized from cruciferous plants, catalyses the last step in the biosynthesis of tryptophan- and phenylalanine-derived glucosinolates. cf. EC 2.8.2.38, aliphatic desulfoglucosinolate sulfotransferase.
analysis/evaluation of desulfoglucosinolate:PAPS sulfotransferase from Arabidopsis thaliana ecotype Col-0 and myrosinase from Brevicoryne brassicae for the biosynthesis of benzyl isothiocyanate by the combined expression of the optimized enzymes in vitro, product identity is confirmed by gas chromatography/mass spectrometry analysis. SOTs from different Arabidopsis ecotypes show different efficiencies
the enzyme is involved in the glucosinolate biosynthesis pathway, glucosinolates play important roles in plant defense against herbivores and microbes, as well as in human nutrition
generation of two chimeric cytochrome P450 enzymes from CYP79A2 and CYP83B1 are constructed and attempted to be functionally expressed in Escherichia coli strain BL21(DE3). The Escherichia coli cystathionine beta-lyase is used to replace the plant-derived CS lyase whose active form cannot be expressed in Escherichia coli. Recomstruction of the BITC biosynthesis pathway from plants in the bacteria, involving CYP79A2 and CYP83B1, and the glutathione S-transferase, gamma-glutamyl peptidase, C-S lyase, glucosyltransferase, desulfoglucosinolate:PAPS sulfotransferase, and myrosinase are encoded by genes GSTF, GGP1, SUR1, UGT74B1, SOT16, and MYR, respectively, overview. Expression of codon-optimized sulfotransferase gene SOT16 from Arabidopsis thaliana fails because of the formation of inclusion bodies, SOT16 is successfully replaced for SOT18 (EC 2.8.2.38)
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
co-expression of the enzyme with myrosinase from Brevicoryne brassicae in Escherichia coli, expression of codon-optimized sulfotransferase gene SOT16 from Arabidopsis thaliana fails because of the formation of inclusion bodies
DNA and amino acid sequence determination and analysis, gene structure, phylogenetic tree, genomic study of genes involved in glucosinolate biosynthesis, comparison with Brassica rapa genome, overview
Partial purification and characterization of a 3'-phosphoadenosine 5'-phosphosulfate: desulfoglucosinolate sulfotransferase from cress (Lepidium sativum)
Biosynthesis of the high-value plant secondary product benzyl isothiocyanate via functional expression of multiple heterologous enzymes in Escherichia coli