Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli M15 | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
ecotype C24, ecotype Col-0 | - |
Purification (Comment) | Organism |
---|---|
nickel-nitrilotriacetic acid-agarose | Arabidopsis thaliana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
isoenzyme AtST5a: best substrate: indole-3-methyl-desulfoglucosinolate, other substrates: benzyl-desulfoglucosinolate, 4-methylthiobutyl-desulfoglucosinolate, 4-methoxyindole-3-methyl-desulfoglucosinolate, N-methoxyindole-3-methyl-desulfoglucosinolate. pH 7.0, 30°C | Arabidopsis thaliana |
additional information | - |
isoenzyme AtST5b: best substrate: 7-methylthioheptyl-desulfoglucosinolate, other substrates: 8-methylthiooctyl-desulfoglucosinolate, 4-methylthiobutyl-desulfoglucosinolate, benzyl-desulfoglucosinolate, indole-3-methyl-desulfoglucosinolate, 8-methylsulfinyloctyl-desulfoglucosinolate, 3-butenyl-desulfoglucosinolate. pH 7.0, 30°C | Arabidopsis thaliana |
additional information | - |
isoenzyme AtST5c: best substrate: 8-methylthiooctyl-desulfoglucosinolate, other substrates: 7-methylthioheptyl-desulfoglucosinolate, benzyl-desulfoglucosinolate, 3-butenyl-desulfoglucosinolate, 4-methylthiobutyl-desulfoglucosinolate, 8-methylsulfinyloctyl-desulfoglucosinolate, 4-methylsulfinylbutyl-desulfoglucosinolate. pH 7.0, 30°C | Arabidopsis thaliana |