Information on EC 2.8.2.24 - aromatic desulfoglucosinolate sulfotransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.8.2.24
-
RECOMMENDED NAME
GeneOntology No.
aromatic desulfoglucosinolate sulfotransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3'-phosphoadenylyl sulfate + desulfoglucotropeolin = adenosine 3',5'-bisphosphate + glucotropeolin
show the reaction diagram
3'-phosphoadenylyl sulfate + indolylmethyl-desulfoglucosinolate = adenosine 3',5'-bisphosphate + glucobrassicin
show the reaction diagram
(2)
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-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sulfate group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
glucosinolate biosynthesis from phenylalanine
-
-
glucosinolate biosynthesis from tryptophan
-
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glucosinolate biosynthesis from tyrosine
-
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Tryptophan metabolism
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Glucosinolate biosynthesis
-
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Biosynthesis of secondary metabolites
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SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:aromatic desulfoglucosinolate sulfotransferase
Involved with EC 2.4.1.195 thiohydroximate beta-D-glucosyltransferase, in the biosynthesis of thioglycosides in cruciferous plants.
CAS REGISTRY NUMBER
COMMENTARY hide
121479-85-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
rapeseed, cv. Westar
-
-
Manually annotated by BRENDA team
cv. 526
-
-
Manually annotated by BRENDA team
savoy cabbage
-
-
Manually annotated by BRENDA team
at least 12 paralogues of desulfoglucosinolate sulfotransferase
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-
Manually annotated by BRENDA team
no.5089, curled cress
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenosine-5'-phosphosulfate + desulfobenzylglucosinolate
adenosine 3',5'-bisphosphate + benzylglucosinolate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + desulfo-p-hydroxy-benzylglucosinolate
adenosine 3',5'-bisphosphate + p-hydroxy-benzylglucosinolate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + desulfoallylglucosinolate
adenosine 3',5'-bisphosphate + allylglucosinolate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate
adenosine 3',5'-bisphosphate + benzylglucosinolate
show the reaction diagram
3'-phosphoadenylylsulfate + desulfobenzylglucosinolate
adenosine 3',5'-bisphosphate + benzylglucosinolate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylylsulfate + desulfobenzylglucosinolate
adenosine 3',5'-bisphosphate + benzylglucosinolate
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,2'-dipyridyl
-
26% inhibition at 10 mM
adenosine-3',5'-bisphosphate
Ag+
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13% inhibition at 1 mM
Co2+
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at low concentration
DTNB
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2-mercaptoethanol protects
EDTA
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15% inhibition at 10 mM
iodoacetamide
iodoacetic acid
Iodosobenzoic acid
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-
Mn2+
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inhibitory at 10 mM, stimulating at 1 mM
N-ethylmaleimide
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2-mercaptoethanol protects
N-Methylmaleimide
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N-Pyrenylmaleimide
-
-
Ni2+
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30% inhibition at 1 mM
o-phenanthroline
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34% inhibition at 10 mM
p-Chloromercuriphenylsulfonic acid
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2-mercaptoethanol protects
Pb(NO3)2
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25% inhibition at 1 mM
Phenylmercuriacetate
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phosphoadenosine-5'-phosphate
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-
-
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.05 - 0.1
3'-phosphoadenosine-5'-phosphosulfate
0.00078 - 0.06
3'-phosphoadenylylsulfate
0.67
desulfo-p-hydroxy-benzylglucosinolate
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pH 9.0, 30°C
0.0065
desulfoallylglucosinolate
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pH 9.0, 30°C
0.0023 - 0.1
desulfobenzylglucosinolate
additional information
additional information
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0004
adenosine-3',5'-bisphosphate
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pH 8.5-9.0, 30°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0000329
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partially purified enzyme
0.935
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purified enzyme
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.5 - 9
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maximal activity in Tris-HCl buffer
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 9.5
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about 65% of maximal activity at pH 7.0 and pH 9.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 40
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about 85% of maximal activity at 25°C and about half-maximal activity at 40°C
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
derived from hypocotyls
Manually annotated by BRENDA team
additional information
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expression of the gene paralogues is not tissue-specific but varies greatly depending on the tissue type. The expression is also developmentally regulated for some paralogues but not for others
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
31000
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gel filtration
39200
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ecotype Co10, isoenzyme AtSOT16, calculated
39900
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ecotype Co10, isoenzyme AtSOT17, calculated
40500
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ecotype Co10, isoenzyme AtSOT18, calculated
44000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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1 * 31000-55000, SDS-PAGE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
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t1/2: 1 h, 4°C
643823
8
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stable above
643823
10.5
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t1/2: 1 h, 4°C
643823
additional information
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activity loss due to instability at pH values near the isoelectric point 5.2
643824
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
-
t1/2: 1 h, 20 mM Tris-HCl buffer, pH 7.5, 14 mM 2-mercaptoethanol
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
bovine serum albumin, required for stabilizing
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dilution inactivates
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MgCl2, 2-mercaptoethanol, DTT and GSH do not stabilize
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 0.25 M sucrose and bovine serum albumin, 6 months
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-20°C, 20 mM Tris buffer, pH 7.5, 14 mM 2-mercaptoethanol, 10% glycerol, at least 2 months
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4°C, 14 mM 2-mercaptoethanol, t1/2: about 2 weeks
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4°C, more than 50% loss of activity within 48 h
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
nickel-nitrilotriacetic acid-agarose
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partial, 230fold
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persistently co-purified with EC 2.4.1.195
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, gene structure, phylogenetic tree, genomic study of genes involved in glucosinolate biosynthesis, comparison with Arabidopsis thaliana genome, overview
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DNA and amino acid sequence determination and analysis, gene structure, phylogenetic tree, genomic study of genes involved in glucosinolate biosynthesis, comparison with Brassica rapa genome, overview
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expression in Escherichia coli M15
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
G301D
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isoenzyme AtSOT18 C24, 25times higher specific activity with desulfobenzylglucosinolate
N339K
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to date no purification of the protein under native conditions