General Stability | Organism |
---|---|
bovine serum albumin, required for stabilizing | Lepidium sativum |
dilution inactivates | Lepidium sativum |
MgCl2, 2-mercaptoethanol, DTT and GSH do not stabilize | Lepidium sativum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-dipyridyl | 26% inhibition at 10 mM | Lepidium sativum | |
adenosine-3',5'-bisphosphate | product inhibition | Lepidium sativum | |
Ag+ | 13% inhibition at 1 mM | Lepidium sativum | |
Cu2+ | 14% inhibition at 1 mM | Lepidium sativum | |
DTNB | 2-mercaptoethanol protects | Lepidium sativum | |
EDTA | 15% inhibition at 10 mM | Lepidium sativum | |
iodoacetamide | weak inhibition | Lepidium sativum | |
iodoacetic acid | weak inhibition | Lepidium sativum | |
additional information | no inhibition by FeCl3, FeCl2, CaCl2, CoCl2 | Lepidium sativum | |
N-ethylmaleimide | 2-mercaptoethanol protects | Lepidium sativum | |
Ni2+ | 30% inhibition at 1 mM | Lepidium sativum | |
o-phenanthroline | 34% inhibition at 10 mM | Lepidium sativum | |
p-chloromercuriphenylsulfonic acid | 2-mercaptoethanol protects | Lepidium sativum | |
Pb(NO3)2 | 25% inhibition at 1 mM | Lepidium sativum | |
Zn2+ | 46% inhibition at 1 mM | Lepidium sativum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic studies | Lepidium sativum | |
0.0065 | - |
desulfoallylglucosinolate | pH 9.0, 30°C | Lepidium sativum | |
0.06 | - |
3'-phosphoadenylylsulfate | pH 9.0, 30°C | Lepidium sativum | |
0.082 | - |
desulfobenzylglucosinolate | pH 9.0, 30°C | Lepidium sativum | |
0.67 | - |
desulfo-p-hydroxy-benzylglucosinolate | pH 9.0, 30°C | Lepidium sativum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 51% activation at 10 mM | Lepidium sativum | |
Mn2+ | 23% activation at 1 mM, 10 mM inhibits by 53% | Lepidium sativum | |
additional information | not affected by FeCl3, FeCl2, CaCl2, CoCl2 | Lepidium sativum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
gel filtration | Lepidium sativum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylylsulfate + desulfobenzylglucosinolate | Lepidium sativum | - |
adenosine 3',5'-bisphosphate + benzylglucosinolate | - |
? | |
additional information | Lepidium sativum | involved in the biosynthesis of glucosinolates | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Brassica rapa subsp. oleifera | - |
- |
- |
Lepidium sativum | - |
no.5089, curled cress | - |
Sinapis alba | - |
- |
- |
Tropaeolum majus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
40.1fold | Lepidium sativum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
3'-phosphoadenylyl sulfate + desulfoglucotropeolin = adenosine 3',5'-bisphosphate + glucotropeolin | mechanism | Lepidium sativum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | etiolated | Arabidopsis thaliana | - |
seedling | etiolated | Lepidium sativum | - |
seedling | etiolated | Brassica rapa subsp. oleifera | - |
seedling | etiolated | Sinapis alba | - |
seedling | etiolated | Tropaeolum majus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.935 | - |
purified enzyme | Lepidium sativum |
Storage Stability | Organism |
---|---|
-20°C, 0.25 M sucrose and bovine serum albumin, 6 months | Lepidium sativum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + desulfo-p-hydroxy-benzylglucosinolate | - |
Lepidium sativum | adenosine 3',5'-bisphosphate + p-hydroxy-benzylglucosinolate | - |
? | |
3'-phosphoadenylyl sulfate + desulfoallylglucosinolate | - |
Lepidium sativum | adenosine 3',5'-bisphosphate + allylglucosinolate | - |
? | |
3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate | i.e. 3'-phosphoadenosine 5'phosphosulfate, i.e. PAPS | Lepidium sativum | adenosine 3',5'-bisphosphate + benzylglucosinolate | - |
? | |
3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate | absolute specificity for desulfoglucosinolate structure | Arabidopsis thaliana | adenosine 3',5'-bisphosphate + benzylglucosinolate | - |
? | |
3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate | absolute specificity for desulfoglucosinolate structure | Lepidium sativum | adenosine 3',5'-bisphosphate + benzylglucosinolate | - |
? | |
3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate | absolute specificity for desulfoglucosinolate structure | Brassica rapa subsp. oleifera | adenosine 3',5'-bisphosphate + benzylglucosinolate | - |
? | |
3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate | absolute specificity for desulfoglucosinolate structure | Sinapis alba | adenosine 3',5'-bisphosphate + benzylglucosinolate | - |
? | |
3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate | absolute specificity for desulfoglucosinolate structure | Tropaeolum majus | adenosine 3',5'-bisphosphate + benzylglucosinolate | - |
? | |
3'-phosphoadenylylsulfate + desulfobenzylglucosinolate | - |
Lepidium sativum | adenosine 3',5'-bisphosphate + benzylglucosinolate | - |
? | |
additional information | no acceptors are quercetin, rutin, kaempferol, p-coumaric acid, ferulic acid, caffeic acid, phenylacetaldoxime | Lepidium sativum | ? | - |
? | |
additional information | involved in the biosynthesis of glucosinolates | Lepidium sativum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 31000-55000, SDS-PAGE | Lepidium sativum |
Synonyms | Comment | Organism |
---|---|---|
ST | - |
Lepidium sativum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
maximal activity in Tris-HCl buffer | Lepidium sativum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9.5 | about 65% of maximal activity at pH 7.0 and pH 9.5 | Lepidium sativum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
activity loss due to instability at pH values near the isoelectric point 5.2 | Lepidium sativum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Lepidium sativum | - |
- |
5.2 |