Partial purification and characterization of a 3'-phosphoadenosine 5'-phosphosulfate: desulfoglucosinolate sulfotransferase from cress (Lepidium sativum)

Glendening, T.M.; Poulton, J.E.; Plant Physiol. 94, 811-816 (1990)

Data extracted from this reference:

Inhibitors

Commentary

Organism

Structure

2,2'-dipyridyl

26% inhibition at 10 mM

Lepidium sativum

adenosine-3',5'-bisphosphate

product inhibition

Lepidium sativum

Ag+

13% inhibition at 1 mM

Lepidium sativum

Cu2+

14% inhibition at 1 mM

Lepidium sativum

DTNB

2-mercaptoethanol protects

Lepidium sativum

EDTA

15% inhibition at 10 mM

Lepidium sativum

iodoacetamide

weak inhibition

Lepidium sativum

iodoacetic acid

weak inhibition

Lepidium sativum

additional information

no inhibition by FeCl3, FeCl2, CaCl2, CoCl2

Lepidium sativum

N-ethylmaleimide

2-mercaptoethanol protects

Lepidium sativum

Ni2+

30% inhibition at 1 mM

Lepidium sativum

o-phenanthroline

34% inhibition at 10 mM

Lepidium sativum

p-chloromercuriphenylsulfonic acid

2-mercaptoethanol protects

Lepidium sativum

Pb(NO3)2

25% inhibition at 1 mM

Lepidium sativum

Zn2+

46% inhibition at 1 mM

Lepidium sativum

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

additional information

-

additional information

kinetic studies

Lepidium sativum

0.0065

-

desulfoallylglucosinolate

pH 9.0, 30°C

Lepidium sativum

0.06

-

3'-phosphoadenylylsulfate

pH 9.0, 30°C

Lepidium sativum

0.082

-

desulfobenzylglucosinolate

pH 9.0, 30°C

Lepidium sativum

0.67

-

desulfo-p-hydroxy-benzylglucosinolate

pH 9.0, 30°C

Lepidium sativum

Metals/Ions

Commentary

Organism

Structure

Mg2+

51% activation at 10 mM

Lepidium sativum

Mn2+

23% activation at 1 mM, 10 mM inhibits by 53%

Lepidium sativum

additional information

not affected by FeCl3, FeCl2, CaCl2, CoCl2

Lepidium sativum

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

3'-phosphoadenylylsulfate + desulfobenzylglucosinolate

Lepidium sativum

-

adenosine 3',5'-bisphosphate + benzylglucosinolate

-

Lepidium sativum

?

additional information

Lepidium sativum

involved in the biosynthesis of glucosinolates

?

-

-

-

Reaction

Commentary

Organism

Reaction ID

3'-phosphoadenylyl sulfate + desulfoglucotropeolin = adenosine 3',5'-bisphosphate + glucotropeolin

mechanism

Lepidium sativum

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

3'-phosphoadenylyl sulfate + desulfo-p-hydroxy-benzylglucosinolate

-

643824

Lepidium sativum

adenosine 3',5'-bisphosphate + p-hydroxy-benzylglucosinolate

-

-

-

?

3'-phosphoadenylyl sulfate + desulfoallylglucosinolate

-

643824

Lepidium sativum

adenosine 3',5'-bisphosphate + allylglucosinolate

-

-

-

?

3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate

i.e. 3'-phosphoadenosine 5'phosphosulfate, i.e. PAPS

643824

Lepidium sativum

adenosine 3',5'-bisphosphate + benzylglucosinolate

-

643824

Lepidium sativum

?

3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate

absolute specificity for desulfoglucosinolate structure

643824

Arabidopsis thaliana

adenosine 3',5'-bisphosphate + benzylglucosinolate

-

643824

Arabidopsis thaliana

?

3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate

absolute specificity for desulfoglucosinolate structure

643824

Lepidium sativum

adenosine 3',5'-bisphosphate + benzylglucosinolate

-

643824

Lepidium sativum

?

3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate

absolute specificity for desulfoglucosinolate structure

643824

Brassica rapa subsp. oleifera

adenosine 3',5'-bisphosphate + benzylglucosinolate

-

643824

Brassica rapa subsp. oleifera

?

3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate

absolute specificity for desulfoglucosinolate structure

643824

Sinapis alba

adenosine 3',5'-bisphosphate + benzylglucosinolate

-

643824

Sinapis alba

?

3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate

absolute specificity for desulfoglucosinolate structure

643824

Tropaeolum majus

adenosine 3',5'-bisphosphate + benzylglucosinolate

-

643824

Tropaeolum majus

?

3'-phosphoadenylylsulfate + desulfobenzylglucosinolate

-

643824

Lepidium sativum

adenosine 3',5'-bisphosphate + benzylglucosinolate

-

643824

Lepidium sativum

?

additional information

no acceptors are quercetin, rutin, kaempferol, p-coumaric acid, ferulic acid, caffeic acid, phenylacetaldoxime

643824

Lepidium sativum

?

-

-

-

-

additional information

involved in the biosynthesis of glucosinolates

643824

Lepidium sativum

?

-

-

-

-

Inhibitors

Commentary

Organism

Structure

2,2'-dipyridyl

26% inhibition at 10 mM

Lepidium sativum

adenosine-3',5'-bisphosphate

product inhibition

Lepidium sativum

Ag+

13% inhibition at 1 mM

Lepidium sativum

Cu2+

14% inhibition at 1 mM

Lepidium sativum

DTNB

2-mercaptoethanol protects

Lepidium sativum

EDTA

15% inhibition at 10 mM

Lepidium sativum

iodoacetamide

weak inhibition

Lepidium sativum

iodoacetic acid

weak inhibition

Lepidium sativum

additional information

no inhibition by FeCl3, FeCl2, CaCl2, CoCl2

Lepidium sativum

N-ethylmaleimide

2-mercaptoethanol protects

Lepidium sativum

Ni2+

30% inhibition at 1 mM

Lepidium sativum

o-phenanthroline

34% inhibition at 10 mM

Lepidium sativum

p-chloromercuriphenylsulfonic acid

2-mercaptoethanol protects

Lepidium sativum

Pb(NO3)2

25% inhibition at 1 mM

Lepidium sativum

Zn2+

46% inhibition at 1 mM

Lepidium sativum

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

additional information

-

additional information

kinetic studies

Lepidium sativum

0.0065

-

desulfoallylglucosinolate

pH 9.0, 30°C

Lepidium sativum

0.06

-

3'-phosphoadenylylsulfate

pH 9.0, 30°C

Lepidium sativum

0.082

-

desulfobenzylglucosinolate

pH 9.0, 30°C

Lepidium sativum

0.67

-

desulfo-p-hydroxy-benzylglucosinolate

pH 9.0, 30°C

Lepidium sativum

Metals/Ions

Commentary

Organism

Structure

Mg2+

51% activation at 10 mM

Lepidium sativum

Mn2+

23% activation at 1 mM, 10 mM inhibits by 53%

Lepidium sativum

additional information

not affected by FeCl3, FeCl2, CaCl2, CoCl2

Lepidium sativum

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

3'-phosphoadenylylsulfate + desulfobenzylglucosinolate

Lepidium sativum

-

adenosine 3',5'-bisphosphate + benzylglucosinolate

-

Lepidium sativum

?

additional information

Lepidium sativum

involved in the biosynthesis of glucosinolates

?

-

-

-

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

3'-phosphoadenylyl sulfate + desulfo-p-hydroxy-benzylglucosinolate

-

643824

Lepidium sativum

adenosine 3',5'-bisphosphate + p-hydroxy-benzylglucosinolate

-

-

-

?

3'-phosphoadenylyl sulfate + desulfoallylglucosinolate

-

643824

Lepidium sativum

adenosine 3',5'-bisphosphate + allylglucosinolate

-

-

-

?

3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate

i.e. 3'-phosphoadenosine 5'phosphosulfate, i.e. PAPS

643824

Lepidium sativum

adenosine 3',5'-bisphosphate + benzylglucosinolate

-

643824

Lepidium sativum

?

3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate

absolute specificity for desulfoglucosinolate structure

643824

Arabidopsis thaliana

adenosine 3',5'-bisphosphate + benzylglucosinolate

-

643824

Arabidopsis thaliana

?

3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate

absolute specificity for desulfoglucosinolate structure

643824

Lepidium sativum

adenosine 3',5'-bisphosphate + benzylglucosinolate

-

643824

Lepidium sativum

?

3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate

absolute specificity for desulfoglucosinolate structure

643824

Brassica rapa subsp. oleifera

adenosine 3',5'-bisphosphate + benzylglucosinolate

-

643824

Brassica rapa subsp. oleifera

?

3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate

absolute specificity for desulfoglucosinolate structure

643824

Sinapis alba

adenosine 3',5'-bisphosphate + benzylglucosinolate

-

643824

Sinapis alba

?

3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate

absolute specificity for desulfoglucosinolate structure

643824

Tropaeolum majus

adenosine 3',5'-bisphosphate + benzylglucosinolate

-

643824

Tropaeolum majus

?

3'-phosphoadenylylsulfate + desulfobenzylglucosinolate

-

643824

Lepidium sativum

adenosine 3',5'-bisphosphate + benzylglucosinolate

-

643824

Lepidium sativum

?

additional information

no acceptors are quercetin, rutin, kaempferol, p-coumaric acid, ferulic acid, caffeic acid, phenylacetaldoxime

643824

Lepidium sativum

?

-

-

-

-

additional information

involved in the biosynthesis of glucosinolates

643824

Lepidium sativum

?

-

-

-

-

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

703650

Zang

Genome-wide identification of ...

Arabidopsis thaliana, Brassica rapa

FEBS J.

276

3559-3574

2009

-

-

2

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-

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2

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1

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2

2

-

-

-

661715

Klein

The three desulfoglucosinolate ...

Arabidopsis thaliana

FEBS J.

273

122-136

2006

-

-

-

-

2

-

1

7

1

-

3

-

-

1

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1

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1

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1

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1

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1

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1

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7

1

-

3

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1

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1

-

1

-

1

-

-

-

1

-

-

-

-

-

-

-

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-

662305

Piotrowski

Desulfoglucosinolate sulfotran ...

Arabidopsis thaliana

J. Biol. Chem.

279

50717-50725

2004

-

-

1

-

-

-

-

-

-

-

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1

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1

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1

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-

-

-

643823

Jain

-

Persistent co-purification of ...

Brassica juncea

J. Plant Physiol.

136

356-361

1990

-

-

-

-

-

-

-

-

1

-

-

2

-

1

-

-

1

-

-

1

-

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3

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-

-

2

-

-

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3

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-

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1

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1

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1

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3

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3

-

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-

-

-

-

-

643824

Glendening

Partial purification and chara ...

Arabidopsis thaliana, Brassica rapa subsp. oleifera, Lepidium sativum, Sinapis alba, Tropaeolum majus

Plant Physiol.

94

811-816

1990

-

-

-

-

-

3

15

5

-

3

1

2

-

5

-

-

1

1

-

5

1

1

11

1

1

-

-

-

-

1

1

1

-

-

1

-

-

-

-

-

-

-

3

-

15

-

5

-

3

1

2

-

-

-

1

-

5

1

1

11

1

-

-

-

-

1

1

1

1

-

-

-

-

-

-

643825

Jain

-

Purification and properties of ...

Brassica juncea

Phytochemistry

29

1425-1428

1990

-

-

-

-

-

-

13

2

-

3

1

3

-

1

-

-

1

-

-

1

1

3

5

-

-

1

1

1

-

1

-

-

-

1

1

-

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-

-

-

-

-

-

-

13

1

2

-

3

1

3

-

-

-

1

-

1

1

3

5

-

1

1

1

-

1

-

-

1

-

-

-

-

-

-

643821

Jain

A radioassay of enzymes cataly ...

Brassica juncea, Brassica napus, Brassica nigra, Brassica oleracea, Brassica rapa subsp. oleifera

Anal. Biochem.

178

137-140

1989

-

-

-

-

-

-

-

-

-

-

-

-

-

7

-

-

1

-

-

5

5

-

5

-

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1

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5

5

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5

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-

-

-

-

-

-

-

-

-

643822

Jain

-

Distribution of enzymes cataly ...

Brassica juncea

Plant Sci.

64

25-29

1989

-

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-

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1

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