BRENDA - Enzyme Database
show all sequences of 2.8.2.24

Partial purification and characterization of a 3'-phosphoadenosine 5'-phosphosulfate: desulfoglucosinolate sulfotransferase from cress (Lepidium sativum)

Glendening, T.M.; Poulton, J.E.; Plant Physiol. 94, 811-816 (1990)

Data extracted from this reference:

General Stability
General Stability
Organism
bovine serum albumin, required for stabilizing
Lepidium sativum
dilution inactivates
Lepidium sativum
MgCl2, 2-mercaptoethanol, DTT and GSH do not stabilize
Lepidium sativum
Inhibitors
Inhibitors
Commentary
Organism
Structure
2,2'-dipyridyl
26% inhibition at 10 mM
Lepidium sativum
adenosine-3',5'-bisphosphate
product inhibition
Lepidium sativum
Ag+
13% inhibition at 1 mM
Lepidium sativum
Cu2+
14% inhibition at 1 mM
Lepidium sativum
DTNB
2-mercaptoethanol protects
Lepidium sativum
EDTA
15% inhibition at 10 mM
Lepidium sativum
iodoacetamide
weak inhibition
Lepidium sativum
iodoacetic acid
weak inhibition
Lepidium sativum
additional information
no inhibition by FeCl3, FeCl2, CaCl2, CoCl2
Lepidium sativum
N-ethylmaleimide
2-mercaptoethanol protects
Lepidium sativum
Ni2+
30% inhibition at 1 mM
Lepidium sativum
o-phenanthroline
34% inhibition at 10 mM
Lepidium sativum
p-chloromercuriphenylsulfonic acid
2-mercaptoethanol protects
Lepidium sativum
Pb(NO3)2
25% inhibition at 1 mM
Lepidium sativum
Zn2+
46% inhibition at 1 mM
Lepidium sativum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic studies
Lepidium sativum
0.0065
-
desulfoallylglucosinolate
pH 9.0, 30C
Lepidium sativum
0.06
-
3'-phosphoadenylylsulfate
pH 9.0, 30C
Lepidium sativum
0.082
-
desulfobenzylglucosinolate
pH 9.0, 30C
Lepidium sativum
0.67
-
desulfo-p-hydroxy-benzylglucosinolate
pH 9.0, 30C
Lepidium sativum
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
51% activation at 10 mM
Lepidium sativum
Mn2+
23% activation at 1 mM, 10 mM inhibits by 53%
Lepidium sativum
additional information
not affected by FeCl3, FeCl2, CaCl2, CoCl2
Lepidium sativum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31000
-
gel filtration
Lepidium sativum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3'-phosphoadenylylsulfate + desulfobenzylglucosinolate
Lepidium sativum
-
adenosine 3',5'-bisphosphate + benzylglucosinolate
-
Lepidium sativum
?
additional information
Lepidium sativum
involved in the biosynthesis of glucosinolates
?
-
-
-
Organism
Organism
UniProt
Commentary
Textmining
Arabidopsis thaliana
-
-
-
Brassica rapa subsp. oleifera
-
-
-
Lepidium sativum
-
no.5089, curled cress
-
Sinapis alba
-
-
-
Tropaeolum majus
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
40.1fold
Lepidium sativum
Reaction
Reaction
Commentary
Organism
Reaction ID
3'-phosphoadenylyl sulfate + desulfoglucotropeolin = adenosine 3',5'-bisphosphate + glucotropeolin
mechanism
Lepidium sativum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
seedling
etiolated
Arabidopsis thaliana
-
seedling
etiolated
Brassica rapa subsp. oleifera
-
seedling
etiolated
Lepidium sativum
-
seedling
etiolated
Sinapis alba
-
seedling
etiolated
Tropaeolum majus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.935
-
purified enzyme
Lepidium sativum
Storage Stability
Storage Stability
Organism
-20C, 0.25 M sucrose and bovine serum albumin, 6 months
Lepidium sativum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3'-phosphoadenylyl sulfate + desulfo-p-hydroxy-benzylglucosinolate
-
643824
Lepidium sativum
adenosine 3',5'-bisphosphate + p-hydroxy-benzylglucosinolate
-
-
-
?
3'-phosphoadenylyl sulfate + desulfoallylglucosinolate
-
643824
Lepidium sativum
adenosine 3',5'-bisphosphate + allylglucosinolate
-
-
-
?
3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate
i.e. 3'-phosphoadenosine 5'phosphosulfate, i.e. PAPS
643824
Lepidium sativum
adenosine 3',5'-bisphosphate + benzylglucosinolate
-
643824
Lepidium sativum
?
3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate
absolute specificity for desulfoglucosinolate structure
643824
Arabidopsis thaliana
adenosine 3',5'-bisphosphate + benzylglucosinolate
-
643824
Arabidopsis thaliana
?
3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate
absolute specificity for desulfoglucosinolate structure
643824
Lepidium sativum
adenosine 3',5'-bisphosphate + benzylglucosinolate
-
643824
Lepidium sativum
?
3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate
absolute specificity for desulfoglucosinolate structure
643824
Brassica rapa subsp. oleifera
adenosine 3',5'-bisphosphate + benzylglucosinolate
-
643824
Brassica rapa subsp. oleifera
?
3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate
absolute specificity for desulfoglucosinolate structure
643824
Sinapis alba
adenosine 3',5'-bisphosphate + benzylglucosinolate
-
643824
Sinapis alba
?
3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate
absolute specificity for desulfoglucosinolate structure
643824
Tropaeolum majus
adenosine 3',5'-bisphosphate + benzylglucosinolate
-
643824
Tropaeolum majus
?
3'-phosphoadenylylsulfate + desulfobenzylglucosinolate
-
643824
Lepidium sativum
adenosine 3',5'-bisphosphate + benzylglucosinolate
-
643824
Lepidium sativum
?
additional information
no acceptors are quercetin, rutin, kaempferol, p-coumaric acid, ferulic acid, caffeic acid, phenylacetaldoxime
643824
Lepidium sativum
?
-
-
-
-
additional information
involved in the biosynthesis of glucosinolates
643824
Lepidium sativum
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 31000-55000, SDS-PAGE
Lepidium sativum
Synonyms
Synonyms
Commentary
Organism
ST
-
Lepidium sativum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
maximal activity in Tris-HCl buffer
Lepidium sativum
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
9.5
about 65% of maximal activity at pH 7.0 and pH 9.5
Lepidium sativum
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
additional information
-
activity loss due to instability at pH values near the isoelectric point 5.2
Lepidium sativum
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Lepidium sativum
-
-
5.2
General Stability (protein specific)
General Stability
Organism
bovine serum albumin, required for stabilizing
Lepidium sativum
dilution inactivates
Lepidium sativum
MgCl2, 2-mercaptoethanol, DTT and GSH do not stabilize
Lepidium sativum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2,2'-dipyridyl
26% inhibition at 10 mM
Lepidium sativum
adenosine-3',5'-bisphosphate
product inhibition
Lepidium sativum
Ag+
13% inhibition at 1 mM
Lepidium sativum
Cu2+
14% inhibition at 1 mM
Lepidium sativum
DTNB
2-mercaptoethanol protects
Lepidium sativum
EDTA
15% inhibition at 10 mM
Lepidium sativum
iodoacetamide
weak inhibition
Lepidium sativum
iodoacetic acid
weak inhibition
Lepidium sativum
additional information
no inhibition by FeCl3, FeCl2, CaCl2, CoCl2
Lepidium sativum
N-ethylmaleimide
2-mercaptoethanol protects
Lepidium sativum
Ni2+
30% inhibition at 1 mM
Lepidium sativum
o-phenanthroline
34% inhibition at 10 mM
Lepidium sativum
p-chloromercuriphenylsulfonic acid
2-mercaptoethanol protects
Lepidium sativum
Pb(NO3)2
25% inhibition at 1 mM
Lepidium sativum
Zn2+
46% inhibition at 1 mM
Lepidium sativum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic studies
Lepidium sativum
0.0065
-
desulfoallylglucosinolate
pH 9.0, 30C
Lepidium sativum
0.06
-
3'-phosphoadenylylsulfate
pH 9.0, 30C
Lepidium sativum
0.082
-
desulfobenzylglucosinolate
pH 9.0, 30C
Lepidium sativum
0.67
-
desulfo-p-hydroxy-benzylglucosinolate
pH 9.0, 30C
Lepidium sativum
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
51% activation at 10 mM
Lepidium sativum
Mn2+
23% activation at 1 mM, 10 mM inhibits by 53%
Lepidium sativum
additional information
not affected by FeCl3, FeCl2, CaCl2, CoCl2
Lepidium sativum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31000
-
gel filtration
Lepidium sativum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3'-phosphoadenylylsulfate + desulfobenzylglucosinolate
Lepidium sativum
-
adenosine 3',5'-bisphosphate + benzylglucosinolate
-
Lepidium sativum
?
additional information
Lepidium sativum
involved in the biosynthesis of glucosinolates
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
40.1fold
Lepidium sativum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
seedling
etiolated
Arabidopsis thaliana
-
seedling
etiolated
Brassica rapa subsp. oleifera
-
seedling
etiolated
Lepidium sativum
-
seedling
etiolated
Sinapis alba
-
seedling
etiolated
Tropaeolum majus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.935
-
purified enzyme
Lepidium sativum
Storage Stability (protein specific)
Storage Stability
Organism
-20C, 0.25 M sucrose and bovine serum albumin, 6 months
Lepidium sativum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3'-phosphoadenylyl sulfate + desulfo-p-hydroxy-benzylglucosinolate
-
643824
Lepidium sativum
adenosine 3',5'-bisphosphate + p-hydroxy-benzylglucosinolate
-
-
-
?
3'-phosphoadenylyl sulfate + desulfoallylglucosinolate
-
643824
Lepidium sativum
adenosine 3',5'-bisphosphate + allylglucosinolate
-
-
-
?
3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate
i.e. 3'-phosphoadenosine 5'phosphosulfate, i.e. PAPS
643824
Lepidium sativum
adenosine 3',5'-bisphosphate + benzylglucosinolate
-
643824
Lepidium sativum
?
3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate
absolute specificity for desulfoglucosinolate structure
643824
Arabidopsis thaliana
adenosine 3',5'-bisphosphate + benzylglucosinolate
-
643824
Arabidopsis thaliana
?
3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate
absolute specificity for desulfoglucosinolate structure
643824
Lepidium sativum
adenosine 3',5'-bisphosphate + benzylglucosinolate
-
643824
Lepidium sativum
?
3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate
absolute specificity for desulfoglucosinolate structure
643824
Brassica rapa subsp. oleifera
adenosine 3',5'-bisphosphate + benzylglucosinolate
-
643824
Brassica rapa subsp. oleifera
?
3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate
absolute specificity for desulfoglucosinolate structure
643824
Sinapis alba
adenosine 3',5'-bisphosphate + benzylglucosinolate
-
643824
Sinapis alba
?
3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate
absolute specificity for desulfoglucosinolate structure
643824
Tropaeolum majus
adenosine 3',5'-bisphosphate + benzylglucosinolate
-
643824
Tropaeolum majus
?
3'-phosphoadenylylsulfate + desulfobenzylglucosinolate
-
643824
Lepidium sativum
adenosine 3',5'-bisphosphate + benzylglucosinolate
-
643824
Lepidium sativum
?
additional information
no acceptors are quercetin, rutin, kaempferol, p-coumaric acid, ferulic acid, caffeic acid, phenylacetaldoxime
643824
Lepidium sativum
?
-
-
-
-
additional information
involved in the biosynthesis of glucosinolates
643824
Lepidium sativum
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 31000-55000, SDS-PAGE
Lepidium sativum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
maximal activity in Tris-HCl buffer
Lepidium sativum
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
9.5
about 65% of maximal activity at pH 7.0 and pH 9.5
Lepidium sativum
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
additional information
-
activity loss due to instability at pH values near the isoelectric point 5.2
Lepidium sativum
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Lepidium sativum
-
-
5.2
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
703650
Zang
Genome-wide identification of ...
Arabidopsis thaliana, Brassica rapa
FEBS J.
276
3559-3574
2009
-
-
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
661715
Klein
The three desulfoglucosinolate ...
Arabidopsis thaliana
FEBS J.
273
122-136
2006
-
-
-
-
2
-
1
7
1
-
3
-
-
1
-
-
1
-
-
-
1
-
1
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
7
1
-
3
-
-
-
-
1
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
662305
Piotrowski
Desulfoglucosinolate sulfotran ...
Arabidopsis thaliana
J. Biol. Chem.
279
50717-50725
2004
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643823
Jain
-
Persistent co-purification of ...
Brassica juncea
J. Plant Physiol.
136
356-361
1990
-
-
-
-
-
-
-
-
1
-
-
2
-
1
-
-
1
-
-
1
-
-
3
-
-
-
-
2
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
1
-
1
-
-
3
-
-
-
2
-
-
-
3
-
-
-
-
-
-
-
643824
Glendening
Partial purification and chara ...
Arabidopsis thaliana, Brassica rapa subsp. oleifera, Lepidium sativum, Sinapis alba, Tropaeolum majus
Plant Physiol.
94
811-816
1990
-
-
-
-
-
3
15
5
-
3
1
2
-
5
-
-
1
1
-
5
1
1
11
1
1
-
-
-
-
1
1
1
-
-
1
-
-
-
-
-
-
-
3
-
15
-
5
-
3
1
2
-
-
-
1
-
5
1
1
11
1
-
-
-
-
1
1
1
1
-
-
-
-
-
-
643825
Jain
-
Purification and properties of ...
Brassica juncea
Phytochemistry
29
1425-1428
1990
-
-
-
-
-
-
13
2
-
3
1
3
-
1
-
-
1
-
-
1
1
3
5
-
-
1
1
1
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
13
1
2
-
3
1
3
-
-
-
1
-
1
1
3
5
-
1
1
1
-
1
-
-
1
-
-
-
-
-
-
643821
Jain
A radioassay of enzymes cataly ...
Brassica juncea, Brassica napus, Brassica nigra, Brassica oleracea, Brassica rapa subsp. oleifera
Anal. Biochem.
178
137-140
1989
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
1
-
-
5
5
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
5
5
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643822
Jain
-
Distribution of enzymes cataly ...
Brassica juncea
Plant Sci.
64
25-29
1989
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
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-
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-
-
1
-
-
-
-
-
-
-
-
1
-
-
2
-
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-
-
-
-
-
-
-
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-
-
-