Any feedback?
No. of entries
Information on EC 2.7.8.3 - ceramide cholinephosphotransferase
for references in articles please use BRENDA:EC2.7.8.3
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree 2 Transferases
2.7 Transferring phosphorus-containing groups
2.7.8 Transferases for other substituted phosphate groups
2.7.8.3 ceramide cholinephosphotransferase
The enzyme appears in viruses and cellular organisms
- 2.7.8.3
- oligosaccharide
-
n-linked
- mannose
- sphingomyelin
- alpha-mannosidases
-
mannosidase
- cos
-
peptide-bound
-
trimming
-
high-mannose
-
non-cleavable
- 1-deoxymannojirimycin
-
golgi-located
-
sphingomyelinase
- swainsonine
showSynonyms
ceramide cholinephosphotransferase, pc:ceramide cholinephosphotransferase, phosphatidylcholine:ceramide phosphocholinetransferase, more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
PC-ceramide transferase
-
-
-
-
PC:ceramide cholinephosphotransferase
phosphorylcholine-ceramide transferase
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
CDP-choline + a ceramide = CMP + sphingomyelin
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
substituted phospho group transfer
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PATHWAY SOURCE
PATHWAYS
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
CDP-choline:N-acylsphingosine cholinephosphotransferase
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
9026-14-6
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
cytidine diphosphate choline + N-acetyl-DL-threo-1,3-dihydroxy-2-amino-octadecyne
CMP + sphingomyelin
-
Substrates: -
Products: -
Products: -
?
cytidine diphosphate choline + N-octanoyl-DL-threo-1,3-dihydroxy-2-amino-octadecyne
CMP + sphingomyelin
-
Substrates: -
Products: -
Products: -
?
cytidine diphosphate choline + ceramide
CMP + sphingomyelin
-
Substrates: -
Products: -
Products: -
?
cytidine diphosphate choline + ceramide
CMP + sphingomyelin
-
Substrates: -
Products: -
Products: -
?
cytidine diphosphate choline + N-acetyl-DL-threo-trans-sphingosine
CMP + sphingomyelin
-
Substrates: -
Products: -
Products: -
?
cytidine diphosphate choline + N-acetyl-DL-threo-trans-sphingosine
CMP + sphingomyelin
-
Substrates: -
Products: -
Products: -
?
cytidine diphosphate choline + N-acetyl-DL-threo-trans-sphingosine
CMP + sphingomyelin
-
Substrates: -
Products: -
Products: -
?
cytidine diphosphate choline + N-acetyl-DL-threo-trans-sphingosine
CMP + sphingomyelin
-
Substrates: -
Products: -
Products: -
?
cytidine diphosphate choline + N-acetyl-DL-threo-trans-sphingosine
CMP + sphingomyelin
-
Substrates: -
Products: -
Products: -
?
cytidine diphosphate choline + N-acetyl-DL-threo-trans-sphingosine
CMP + sphingomyelin
-
Substrates: -
Products: -
Products: -
?
cytidine diphosphate choline + N-acyl-threo-trans-sphingosine
CMP + sphingomyelin
-
Substrates: -
Products: -
Products: -
?
cytidine diphosphate choline + N-acyl-threo-trans-sphingosine
CMP + sphingomyelin
-
Substrates: -
Products: -
Products: -
?
cytidine diphosphate choline + N-acyl-threo-trans-sphingosine
CMP + sphingomyelin
-
Substrates: -
Products: -
Products: -
?
cytidine diphosphate choline + N-acyl-threo-trans-sphingosine
CMP + sphingomyelin
-
Substrates: sphingomyelin is synthesized predominantly via direct transfer of the phosphorylcholine group from phosphatidylcholine, synthesis of sphingomyelin by the action of CDPcholine:ceramide cholinephosphotransferase occurs only to an extent of 2% in vivo
Products: -
Products: -
?
cytidine diphosphate choline + N-acyl-threo-trans-sphingosine
CMP + sphingomyelin
-
Substrates: -
Products: -
Products: -
?
cytidine diphosphate choline + N-acyl-threo-trans-sphingosine
CMP + sphingomyelin
-
Substrates: biosynthetic pathway of sphingomyelin
Products: -
Products: -
?
cytidine diphosphate choline + N-acyl-threo-trans-sphingosine
CMP + sphingomyelin
-
Substrates: sphingomyelin metabolism
Products: -
Products: -
?
cytidine diphosphate choline + N-acyl-threo-trans-sphingosine
CMP + sphingomyelin
-
Substrates: in vivo the proximal donor of the phosphocholine moiety of sphingomyelin is not CDPcholine but most probably phosphatidylcholine
Products: -
Products: -
?
cytidine diphosphate choline + N-acyl-threo-trans-sphingosine
CMP + sphingomyelin
-
Substrates: biosynthetic pathway of sphingomyelin
Products: -
Products: -
?
cytidine diphosphate choline + N-acyl-threo-trans-sphingosine
CMP + sphingomyelin
-
Substrates: sphingomyelin metabolism
Products: -
Products: -
?
additional information
?
-
-
Substrates: highly specific for CDP-choline, little or no reaction with N-acetyl-DL-threo-dihydrosphingosine and N-acetylphytosphingosine, synthetically prepared UDP-choline, ADP-choline, and GDP-choline shows no detectable activity
Products: -
Products: -
?
additional information
?
-
-
Substrates: highly specific for CDP-choline, active with ceramides containing isomers of sphingosine with the threo configuration of the substituents at carbons 2 and 3, and with trans double bond or triple bond at carbon 4, much less active if the sphingosine moiety has the erythro configuration or a cis double bond
Products: -
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
cytidine diphosphate choline + N-acyl-threo-trans-sphingosine
CMP + sphingomyelin
-
Substrates: -
Products: -
Products: -
?
cytidine diphosphate choline + N-acyl-threo-trans-sphingosine
CMP + sphingomyelin
-
Substrates: -
Products: -
Products: -
?
cytidine diphosphate choline + N-acyl-threo-trans-sphingosine
CMP + sphingomyelin
-
Substrates: -
Products: -
Products: -
?
cytidine diphosphate choline + N-acyl-threo-trans-sphingosine
CMP + sphingomyelin
-
Substrates: sphingomyelin is synthesized predominantly via direct transfer of the phosphorylcholine group from phosphatidylcholine, synthesis of sphingomyelin by the action of CDPcholine:ceramide cholinephosphotransferase occurs only to an extent of 2% in vivo
Products: -
Products: -
?
cytidine diphosphate choline + N-acyl-threo-trans-sphingosine
CMP + sphingomyelin
-
Substrates: -
Products: -
Products: -
?
cytidine diphosphate choline + N-acyl-threo-trans-sphingosine
CMP + sphingomyelin
-
Substrates: biosynthetic pathway of sphingomyelin
Products: -
Products: -
?
cytidine diphosphate choline + N-acyl-threo-trans-sphingosine
CMP + sphingomyelin
-
Substrates: sphingomyelin metabolism
Products: -
Products: -
?
cytidine diphosphate choline + N-acyl-threo-trans-sphingosine
CMP + sphingomyelin
-
Substrates: in vivo the proximal donor of the phosphocholine moiety of sphingomyelin is not CDPcholine but most probably phosphatidylcholine
Products: -
Products: -
?
cytidine diphosphate choline + N-acyl-threo-trans-sphingosine
CMP + sphingomyelin
-
Substrates: biosynthetic pathway of sphingomyelin
Products: -
Products: -
?
cytidine diphosphate choline + N-acyl-threo-trans-sphingosine
CMP + sphingomyelin
-
Substrates: sphingomyelin metabolism
Products: -
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7 - 7.5
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7 - 8.5
-
about 50% of activity maximum at pH 7.0, about 45% of activity maximum at pH 8.5
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
25 - 55
-
about 60% of activity maximum at 25°C, about 70% of activity maximum at 55°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Sribney, M.; Kennedy, E.P.
The enzymatic synthesis of sphingomyelin
J. Biol. Chem.
233
1315-1322
1958
Cavia porcellus, Gallus gallus, Rattus norvegicus, Sus scrofa
brenda
Kennedy, E.P.
Phosphorylcholine-ceramide transferase
Methods Enzymol.
5
486-488
1962
Gallus gallus
-
brenda
Marggraf, W.D.; Anderer, F.A.
Alternative pathways in the biosynthesis of sphingomyelin and the role of phosphatidylcholine, CDPcholine and phosphorylcholine as precursors
Hoppe-Seyler's Z. Physiol. Chem.
355
803-810
1974
Mus musculus
brenda
Stoffel, W.; Melzner, I.
Studies in vitro on the biosynthesis of ceramide and sphingomyelin. A reevaluation of proposed pathways
Hoppe-Seyler's Z. Physiol. Chem.
361
755-771
1980
Rattus norvegicus
brenda
Stoffel, W.; Krueger, E.; Melzner, I.
Studies on the biosynthesis of ceramide. Does the reversed ceramidase reaction yield ceramides?
Hoppe-Seyler's Z. Physiol. Chem.
361
773-779
1980
Rattus norvegicus
brenda
Voelker, D.R.; Kennedy, E.P.
Cellular and enzymic synthesis of sphingomyelin
Biochemistry
21
2753-2759
1982
Mesocricetus auratus, Rattus norvegicus
brenda
Bankov, I.; Barrett, J.
Sphingomyelin synthesis in Ascaridia galli
Int. J. Parasitol.
23
1083-1085
1993
Ascaridia galli
brenda
Nikolova-Karakashian, M.N.; Russell, R.W.; Booth, R.A.; Jenden, D.J.; Merrill, A.H., Jr.
Sphingomyelin metabolism in rat liver after chronic dietary replacement of choline by N-aminodeanol
J. Lipid Res.
38
1764-1770
1997
Rattus norvegicus, Rattus norvegicus Sprague-Dawley
brenda
EXTERNAL LINKS (specific for EC-Number 2.7.8.3)
html completed
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2026.1 (March 2026)
About BRENDA
Social media
Help
Survey
Download
Contribution
Legal
Curated at
Member of
![DSMZ Digital Diversity]()
![Global Core Biodata Resource]()
![ELIXIR Core Data Resource]()
![German Network for Bioinformatics Infrastructure]()
