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Information on EC 2.7.1.B20 - broad specificity nucleoside kinase and Organism(s) Thermoplasma acidophilum and UniProt Accession Q9HJT3

for references in articles please use BRENDA:EC2.7.1.B20
preliminary BRENDA-supplied EC number
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Thermoplasma acidophilum
UNIPROT: Q9HJT3 not found.
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The taxonomic range for the selected organisms is: Thermoplasma acidophilum
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
nucleoside kinase, ta0880, mj0406, pfk-b sugar kinase, bth_i1158, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SYSTEMATIC NAME
IUBMB Comments
NTP:nucleoside 5'-phosphotransferase
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + adenosine
ADP + adenosine 5'-phosphate
show the reaction diagram
catalytic efficiency with cytidine is 22% relative to guanosine
-
-
?
ATP + cytidine
ADP + cytidine 5'-phosphate
show the reaction diagram
catalytic efficiency with cytidine is 45% relative to guanosine
-
-
?
ATP + guanosine
ADP + guanosine 5'-phosphate
show the reaction diagram
phosphoryl donor specificity in order of decreasing Vmax: GTP > ATP > ITP. The catalytic efficiency of GTP is 39% compared to that of ATP
-
-
?
ATP + inosine
ADP + inosine 5'-phosphate
show the reaction diagram
catalytic efficiency with inosine is 62% relative to guanosine
-
-
?
GTP + guanosine
GDP + guanosine 5'-phosphate
show the reaction diagram
phosphoryl donor specificity in order of decreasing Vmax: GTP > ATP > ITP. The catalytic efficiency of GTP is 39% compared to that of ATP
-
-
?
ITP + guanosine
IDP + guanosine 5'-phosphate
show the reaction diagram
phosphoryl donor specificity in order of decreasing Vmax: GTP > ATP > ITP
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
kinase activity is observed with Mg2+ and Co2+ and to a lesser degree with Mn2+
Mg2+
kinase activity is observed with Mg2+ and Co2+ and to a lesser degree with Mn2+
Mn2+
kinase activity is observed with Mg2+ and Co2+ and to a lesser degree with Mn2+
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.001
adenosine
pH 8.0, 22°C
0.0469
ATP
pH 8.0, 22°C, cosubstrate: inosine
0.000411
cytidine
pH 8.0, 22°C
0.185
GTP
pH 8.0, 22°C
0.000208
guanosine
pH 8.0, 22°C
0.000712
Inosine
pH 8.0, 22°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.084
adenosine
pH 8.0, 22°C
0.17
ATP
pH 8.0, 22°C, cosubstrate: inosine
0.064
cytidine
pH 8.0, 22°C
0.27
GTP
pH 8.0, 22°C
0.072
guanosine
pH 8.0, 22°C
0.16
Inosine
pH 8.0, 22°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
74.9
adenosine
pH 8.0, 22°C
3.7
ATP
pH 8.0, 22°C, cosubstrate: inosine
156
cytidine
pH 8.0, 22°C
1.4
GTP
pH 8.0, 22°C
345
guanosine
pH 8.0, 22°C
215
Inosine
pH 8.0, 22°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.2 - 7.8
pH 6.2: about 60% of maximal activity, pH 7.8: about 75% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32804
2 * 32804, calculated from sequence
33000
2 * 33000, SDS-PAGE, crystal structure
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
sitting nanodroplet vapor diffusion method, crystal structure of Ta0880, determined at 1.91 A resolution reveals a dimer with each monomer composed of an alpha/beta/alpha sandwich domain and a smaller lid domain
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Elkin, S.R.; Kumar, A.; Price, C.W.; Columbus, L.
A broad specificity nucleoside kinase from Thermoplasma acidophilum
Proteins
81
568-582
2013
Thermoplasma acidophilum (Q9HJT3), Thermoplasma acidophilum
Manually annotated by BRENDA team