BRENDA - Enzyme Database show
show all sequences of 2.7.1.B20

A broad specificity nucleoside kinase from Thermoplasma acidophilum

Elkin, S.R.; Kumar, A.; Price, C.W.; Columbus, L.; Proteins 81, 568-582 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Thermoplasma acidophilum
Crystallization (Commentary)
Crystallization
Organism
sitting nanodroplet vapor diffusion method, crystal structure of Ta0880, determined at 1.91 A resolution reveals a dimer with each monomer composed of an alpha/beta/alpha sandwich domain and a smaller lid domain
Thermoplasma acidophilum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.000208
-
guanosine
pH 8.0, 22°C
Thermoplasma acidophilum
0.000411
-
cytidine
pH 8.0, 22°C
Thermoplasma acidophilum
0.000712
-
Inosine
pH 8.0, 22°C
Thermoplasma acidophilum
0.001
-
adenosine
pH 8.0, 22°C
Thermoplasma acidophilum
0.0469
-
ATP
pH 8.0, 22°C, cosubstrate: inosine
Thermoplasma acidophilum
0.185
-
GTP
pH 8.0, 22°C
Thermoplasma acidophilum
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
intracellular
-
Thermoplasma acidophilum
5622
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
kinase activity is observed with Mg2+ and Co2+ and to a lesser degree with Mn2+
Thermoplasma acidophilum
Mg2+
kinase activity is observed with Mg2+ and Co2+ and to a lesser degree with Mn2+
Thermoplasma acidophilum
Mn2+
kinase activity is observed with Mg2+ and Co2+ and to a lesser degree with Mn2+
Thermoplasma acidophilum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32804
-
2 * 32804, calculated from sequence
Thermoplasma acidophilum
33000
-
2 * 33000, SDS-PAGE, crystal structure
Thermoplasma acidophilum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thermoplasma acidophilum
Q9HJT3
-
-
Purification (Commentary)
Commentary
Organism
-
Thermoplasma acidophilum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + adenosine
catalytic efficiency with cytidine is 22% relative to guanosine
728780
Thermoplasma acidophilum
ADP + adenosine 5'-phosphate
-
-
-
?
ATP + cytidine
catalytic efficiency with cytidine is 45% relative to guanosine
728780
Thermoplasma acidophilum
ADP + cytidine 5'-phosphate
-
-
-
?
ATP + guanosine
phosphoryl donor specificity in order of decreasing Vmax: GTP > ATP > ITP. The catalytic efficiency of GTP is 39% compared to that of ATP
728780
Thermoplasma acidophilum
ADP + guanosine 5'-phosphate
-
-
-
?
ATP + inosine
catalytic efficiency with inosine is 62% relative to guanosine
728780
Thermoplasma acidophilum
ADP + inosine 5'-phosphate
-
-
-
?
GTP + guanosine
phosphoryl donor specificity in order of decreasing Vmax: GTP > ATP > ITP. The catalytic efficiency of GTP is 39% compared to that of ATP
728780
Thermoplasma acidophilum
GDP + guanosine 5'-phosphate
-
-
-
?
ITP + guanosine
phosphoryl donor specificity in order of decreasing Vmax: GTP > ATP > ITP
728780
Thermoplasma acidophilum
IDP + guanosine 5'-phosphate
-
-
-
?
additional information
negligible enzymatic activity with thymidine, uridine, and 2-deoxyadenosine. Negligible enzymatic activity is observed with UTP and TTP as phosphate donors
728780
Thermoplasma acidophilum
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
2 * 32804, calculated from sequence; 2 * 33000, SDS-PAGE, crystal structure
Thermoplasma acidophilum
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
22
-
assay at
Thermoplasma acidophilum
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.064
-
cytidine
pH 8.0, 22°C
Thermoplasma acidophilum
0.072
-
guanosine
pH 8.0, 22°C
Thermoplasma acidophilum
0.084
-
adenosine
pH 8.0, 22°C
Thermoplasma acidophilum
0.16
-
Inosine
pH 8.0, 22°C
Thermoplasma acidophilum
0.17
-
ATP
pH 8.0, 22°C, cosubstrate: inosine
Thermoplasma acidophilum
0.27
-
GTP
pH 8.0, 22°C
Thermoplasma acidophilum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.6
-
-
Thermoplasma acidophilum
8
-
assay at
Thermoplasma acidophilum
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.2
7.8
pH 6.2: about 60% of maximal activity, pH 7.8: about 75% of maximal activity
Thermoplasma acidophilum
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Thermoplasma acidophilum
Crystallization (Commentary) (protein specific)
Crystallization
Organism
sitting nanodroplet vapor diffusion method, crystal structure of Ta0880, determined at 1.91 A resolution reveals a dimer with each monomer composed of an alpha/beta/alpha sandwich domain and a smaller lid domain
Thermoplasma acidophilum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.000208
-
guanosine
pH 8.0, 22°C
Thermoplasma acidophilum
0.000411
-
cytidine
pH 8.0, 22°C
Thermoplasma acidophilum
0.000712
-
Inosine
pH 8.0, 22°C
Thermoplasma acidophilum
0.001
-
adenosine
pH 8.0, 22°C
Thermoplasma acidophilum
0.0469
-
ATP
pH 8.0, 22°C, cosubstrate: inosine
Thermoplasma acidophilum
0.185
-
GTP
pH 8.0, 22°C
Thermoplasma acidophilum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
intracellular
-
Thermoplasma acidophilum
5622
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
kinase activity is observed with Mg2+ and Co2+ and to a lesser degree with Mn2+
Thermoplasma acidophilum
Mg2+
kinase activity is observed with Mg2+ and Co2+ and to a lesser degree with Mn2+
Thermoplasma acidophilum
Mn2+
kinase activity is observed with Mg2+ and Co2+ and to a lesser degree with Mn2+
Thermoplasma acidophilum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32804
-
2 * 32804, calculated from sequence
Thermoplasma acidophilum
33000
-
2 * 33000, SDS-PAGE, crystal structure
Thermoplasma acidophilum
Purification (Commentary) (protein specific)
Commentary
Organism
-
Thermoplasma acidophilum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + adenosine
catalytic efficiency with cytidine is 22% relative to guanosine
728780
Thermoplasma acidophilum
ADP + adenosine 5'-phosphate
-
-
-
?
ATP + cytidine
catalytic efficiency with cytidine is 45% relative to guanosine
728780
Thermoplasma acidophilum
ADP + cytidine 5'-phosphate
-
-
-
?
ATP + guanosine
phosphoryl donor specificity in order of decreasing Vmax: GTP > ATP > ITP. The catalytic efficiency of GTP is 39% compared to that of ATP
728780
Thermoplasma acidophilum
ADP + guanosine 5'-phosphate
-
-
-
?
ATP + inosine
catalytic efficiency with inosine is 62% relative to guanosine
728780
Thermoplasma acidophilum
ADP + inosine 5'-phosphate
-
-
-
?
GTP + guanosine
phosphoryl donor specificity in order of decreasing Vmax: GTP > ATP > ITP. The catalytic efficiency of GTP is 39% compared to that of ATP
728780
Thermoplasma acidophilum
GDP + guanosine 5'-phosphate
-
-
-
?
ITP + guanosine
phosphoryl donor specificity in order of decreasing Vmax: GTP > ATP > ITP
728780
Thermoplasma acidophilum
IDP + guanosine 5'-phosphate
-
-
-
?
additional information
negligible enzymatic activity with thymidine, uridine, and 2-deoxyadenosine. Negligible enzymatic activity is observed with UTP and TTP as phosphate donors
728780
Thermoplasma acidophilum
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 32804, calculated from sequence; 2 * 33000, SDS-PAGE, crystal structure
Thermoplasma acidophilum
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
22
-
assay at
Thermoplasma acidophilum
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.064
-
cytidine
pH 8.0, 22°C
Thermoplasma acidophilum
0.072
-
guanosine
pH 8.0, 22°C
Thermoplasma acidophilum
0.084
-
adenosine
pH 8.0, 22°C
Thermoplasma acidophilum
0.16
-
Inosine
pH 8.0, 22°C
Thermoplasma acidophilum
0.17
-
ATP
pH 8.0, 22°C, cosubstrate: inosine
Thermoplasma acidophilum
0.27
-
GTP
pH 8.0, 22°C
Thermoplasma acidophilum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.6
-
-
Thermoplasma acidophilum
8
-
assay at
Thermoplasma acidophilum
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.2
7.8
pH 6.2: about 60% of maximal activity, pH 7.8: about 75% of maximal activity
Thermoplasma acidophilum
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.4
-
GTP
pH 8.0, 22°C
Thermoplasma acidophilum
3.7
-
ATP
pH 8.0, 22°C, cosubstrate: inosine
Thermoplasma acidophilum
74.9
-
adenosine
pH 8.0, 22°C
Thermoplasma acidophilum
156
-
cytidine
pH 8.0, 22°C
Thermoplasma acidophilum
215
-
Inosine
pH 8.0, 22°C
Thermoplasma acidophilum
345
-
guanosine
pH 8.0, 22°C
Thermoplasma acidophilum
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.4
-
GTP
pH 8.0, 22°C
Thermoplasma acidophilum
3.7
-
ATP
pH 8.0, 22°C, cosubstrate: inosine
Thermoplasma acidophilum
74.9
-
adenosine
pH 8.0, 22°C
Thermoplasma acidophilum
156
-
cytidine
pH 8.0, 22°C
Thermoplasma acidophilum
215
-
Inosine
pH 8.0, 22°C
Thermoplasma acidophilum
345
-
guanosine
pH 8.0, 22°C
Thermoplasma acidophilum
Other publictions for EC 2.7.1.B20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
728780
Elkin
A broad specificity nucleoside ...
Thermoplasma acidophilum
Proteins
81
568-582
2013
-
-
1
1
-
-
-
6
1
3
2
-
-
4
-
-
1
-
-
-
-
-
7
1
1
-
-
6
2
1
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
6
1
3
2
-
-
-
-
1
-
-
-
-
7
1
1
-
-
6
2
1
-
-
-
-
-
-
6
6
697882
Ota
A novel nucleoside kinase from ...
Burkholderia thailandensis, Burkholderia thailandensis DSM 13276
FEBS J.
275
5865-5872
2008
-
-
1
-
-
-
2
8
-
3
2
-
-
6
-
-
1
-
-
-
-
-
18
1
-
-
3
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
2
-
8
-
3
2
-
-
-
-
1
-
-
-
-
18
1
-
-
3
-
-
-
1
-
-
-
-
-
8
8
726575
Arnfors
Structure of Methanocaldococcu ...
Methanocaldococcus jannaschii, Methanocaldococcus jannaschii DSM 2661
Acta Crystallogr. Sect. D
62
1085-1097
2006
-
-
-
1
-
-
-
-
-
1
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
727430
Hansen
The phosphofructokinase-B (MJ0 ...
Methanocaldococcus jannaschii, Methanocaldococcus jannaschii DSM 2661
Extremophiles
11
105-114
2006
-
-
1
-
-
-
-
4
-
4
2
2
-
9
-
-
1
-
-
-
-
-
16
1
1
-
1
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
4
-
4
2
2
-
-
-
1
-
-
-
-
16
1
1
-
1
-
1
1
-
-
-
1
1
-
-
-
726601
Arnfors
Expression, purification, crys ...
Methanocaldococcus jannaschii
Acta Crystallogr. Sect. F
61
591-594
2005
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-