Ligand NTP

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Basic Ligand Information

Molecular Structure
Picture of NTP (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C5H12O13P3R
NTP
GWRFHFZMNUJGLU-TXICZTDVSA-N
Synonyms:
a nucleoside 5'-triphosphate, a nucleoside triphosphate, nucleoside 5'-triphosphate, nucleoside triphosphate, nucleoside triphosphates, ribonucleoside triphosphate, rNTP

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (31 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
nucleoside triphosphate + AMP = nucleoside diphosphate + ADP
show the reaction diagram
-
NTP + H2O = NDP + phosphate
show the reaction diagram
-
NTP + n NTP = N(pN)n + n diphosphate
show the reaction diagram
-
NTP + 7 dNTP = N(pdN)7 + 7 diphosphate
show the reaction diagram
-
NTP + H2O = NDP + phosphate
show the reaction diagram
-
NTP + H2O = NDP + phosphate
show the reaction diagram
-
NTP + H2O = NDP + phosphate
show the reaction diagram
-

In Vivo Product in Enzyme-catalyzed Reactions (5 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O = ribonucleoside triphosphate + reduced thioredoxin
show the reaction diagram
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-

Substrate in Enzyme-catalyzed Reactions (64 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
nucleoside triphosphate + AMP = nucleoside diphosphate + ADP
show the reaction diagram
-
ribonucleoside triphosphate + DNAn = diphosphate + ?
show the reaction diagram
-
NTP + H2O = NDP + phosphate
show the reaction diagram
-
NTP + N'DP = P1,P4-bis(5'-nucleosyl) tetraphosphate' + phosphate
show the reaction diagram
-
NTP + n NTP = N(pN)n + n diphosphate
show the reaction diagram
-
NTP + 7 dNTP = N(pdN)7 + 7 diphosphate
show the reaction diagram
-
NTP + H2O = NDP + phosphate
show the reaction diagram
-
NTP + H2O = ?
show the reaction diagram
-
NTP + H2O = NDP + phosphate
show the reaction diagram
-
nucleoside triphosphate + H2O = nucleoside diphosphate + phosphate
show the reaction diagram
-
NTP + H2O = NDP + phosphate
show the reaction diagram
-

Product in Enzyme-catalyzed Reactions (9 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O = ribonucleoside triphosphate + reduced thioredoxin
show the reaction diagram
-
-

Enzyme Cofactor/Cosubstrate (1 result)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE

Activator in Enzyme-catalyzed Reactions (1 result)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
stimulate
-

Inhibitor in Enzyme-catalyzed Reactions (12 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
3Dpol is inhibited by NTP concentrations higher than 2 mM
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the strong 3'-5' exonuclease activity of polB1 is inhibited by 50% in the presence of 0.002 mM dNTPs, but remains measurable at up to 0.6 mM dNTPs
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most potent inhibitor, non competitive inhibition
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ribotrinucleotides inhibit the activity with gammaATP
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except ATP
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Enzyme Kinetic Parameters

kcat Value (Turnover Number) (5 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
0.00055
-
pH 8.5, 70°C
0.00013
-
pH 8.5, 70°C

KM Value (7 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
27.5
-
pH 8.0, 60°C, presence of 10 mM Mg2+

Ki Value (1 result)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.09
-
recombinant nonstructural protein 3 analyzed for DNA unwinding rates, data globally fit to a model for substrate inhibition, 22°C, 2 mM MgCl2, 25 nM enzyme, and 5 nM substrate, pH 6.5, initiation by adding each NTP to 0.5 mM

References & Links

Links to other databases for NTP

ChEBI
PubChem
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PubChem