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Information on EC 2.7.1.76 - 2'-deoxyadenosine kinase and Organism(s) Mycoplasma mycoides and UniProt Accession Q93IG4
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2.7.1.76 2'-deoxyadenosine kinaseIUBMB Comments
2'-Deoxyguanosine can also act as acceptor. Possibly identical with EC 2.7.1.74 deoxycytidine kinase.
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Word Map
- 2.7.1.76
- deaminase
- deoxynucleoside
- thymidine
- acidophilus
- 5'-nucleotidase
-
hypoxanthine-guanine
-
ecto-5\'-nucleotidase
- 9-beta-d-arabinofuranosyladenine
- mycoides
- deoxycoformycin
-
2.7.1.20
-
heterotropic
- medicine
The taxonomic range for the selected organisms is: Mycoplasma mycoides
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Synonyms
deoxyadenosine kinase, 2'-deoxyadenosine kinase, dado/dcyd kinase, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
kinase, deoxyadenosine (phosphorylating)
-
-
-
-
purine-deoxyribonucleoside kinase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:2'-deoxyadenosine 5'-phosphotransferase
2'-Deoxyguanosine can also act as acceptor. Possibly identical with EC 2.7.1.74 deoxycytidine kinase.
CAS REGISTRY NUMBER
COMMENTARY
37278-12-9
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + 1-beta-D-arabinofuranosyl cytosine
ADP + 1-beta-D-arabinofuranosyl cytosine 5'-phosphate
3% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 1-beta-D-arabinofuranosyl uracil
ADP + 1-beta-D-arabinofuranosyl uracil 5'-phosphate
0.3% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 2',2'-difluoro-2'-deoxycytidine
ADP + 2',2'-difluoro-2'-deoxycytidine 5'-phosphate
20% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 2',2'-difluoro-2'-deoxyguanosine
ADP + 2',2'-difluoro-2'-deoxyguanosine 5'-phosphate
24% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 2',3'-dideoxyadenosine
ADP + 2',3'-dideoxyadenosine 5'-monophosphate
1.5% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 2',3'-dideoxycytidine
ADP + 2',3'-dideoxycytidine 5'-monophosphate
0.1% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 2',3'-dideoxyguanosine
ADP + 2',3'-dideoxyguanosine 5'-monophosphate
0.5% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 2',3'-dideoxyinoosine
ADP + 2',3'-dideoxyinosine 5'-monophosphate
3.9% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
81% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
97% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 2'-deoxyinosine
ADP + 2'-deoxyinosine 5'-phosphate
116% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
0.01% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 2'-deoxyuridine
ADP + 2'-deoxyuridine 5'-phosphate
0.9% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 2'-fluoro-arabinosyl adenine
ADP + 2'-fluoro-arabinosyl adenine 5'-monophosphate
15% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 2-chloro-2'-deoxyadenosine
ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate
111% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 9-beta-D-arabinofuranosyl adenine
ADP + 9-beta-D-arabinofuranosyl adenine 5'-phosphate
77% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 9-beta-D-arabinofuranosyl guanine
ADP + 9-beta-D-arabinofuranosyl guanine 5'-phosphate
6% of the activity with 2'-deoxyadenosine
-
-
?
ATP + adenosine
ADP + adenosine 5'-monophosphate
7.4% of the activity with 2'-deoxyadenosine
-
-
?
ATP + cytidine
ADP + cytidine 5'-monophosphate
0.1% of the activity with 2'-deoxyadenosine
-
-
?
ATP + guanosine
ADP + guanosine 5'-monophosphate
5.5% of the activity with 2'-deoxyadenosine
-
-
?
ATP + inosine
ADP + inosine monophosphate
5.9% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 1-beta-D-arabinofuranosyl cytosine
ADP + 1-beta-D-arabinofuranosyl cytosine 5'-phosphate
3% relative activity compared to deoxyadenosine
-
-
?
ATP + 2',2'-difluoro-2'-deoxycytidine
ADP + 2',2'-difluoro-2'-deoxycytidine monophosphate
20% relative activity compared to deoxyadenosine
-
-
?
ATP + 2',2'-difluoro-2'-deoxyguanosine
ADP + 2',2'-difluoro-2'-deoxyguanosine monophosphate
24% relative activity compared to deoxyadenosine
-
-
?
ATP + 2',3'-dideoxyadenosine
ADP + 2',3'-dideoxyadenosine monophosphate
1.5% relative activity compared to deoxyadenosine
-
-
?
ATP + 2',3'-dideoxyinosine
ADP + 2',3'-dideoxyinosine 5'-phosphate
3.9% relative activity compared to deoxyadenosine
-
-
?
ATP + 2'-fluoro-arabinosyl adenine
ADP + 2'-fluoro-arabinosyl adenine monophosphate
15% relative activity compared to deoxyadenosine
-
-
?
ATP + 2-chloro-2'-deoxyadenosine
ADP + 2-chloro-2'-deoxyadenosine monophosphate
111% relative activity compared to deoxyadenosine
-
-
?
ATP + 9-beta-D-arabinofuranosyl adenine
ADP + 9-beta-D-arabinofuranosyl adenine 5'-monophosphate
77% relative activity compared to deoxyadenosine
-
-
?
ATP + 9-beta-D-arabinofuranosyl guanine
ADP + 9-beta-D-arabinofuranosyl guanine 5'-monophosphate
6% relative activity compared to deoxyadenosine
-
-
?
ATP + adenosine
ADP + AMP
7.4% relative activity compared to deoxyadenosine
-
-
?
ATP + deoxycytosine
ADP + dCMP
81% relative activity compared to deoxyadenosine
-
-
?
ATP + deoxyinosine
ADP + dIMP
116% relative activity compared to deoxyadenosine
-
-
?
ATP + deoxyuridine
ADP + dUMP
0.9% relative activity compared to deoxyadenosine
-
-
?
ATP + guanosine
ADP + GMP
5.5% relative activity compared to deoxyadenosine
-
-
?
ATP + inosine
ADP + IMP
5.9% relative activity compared to deoxyadenosine
-
-
?
ATP + penciclovir
ADP + ?
1.4% relative activity compared to deoxyadenosine
-
-
?
dATP + deoxyadenosine
dADP + dAMP
2% activity compared to ATP
-
-
?
dCTP + deoxyadenosine
dCDP + dAMP
106% activity compared to ATP
-
-
?
dTTP + deoxyadenosine
dTDP + dAMP
44% activity compared to ATP
-
-
?
ATP + deoxyguanosine
ADP + dGMP
-
approx. 40% of activity with deoxyadenosine
-
?
ATP + deoxyguanosine
ADP + dGMP
97% relative activity compared to deoxyadenosine
-
-
?
CTP + deoxyadenosine
CDP + dAMP
57% activity compared to ATP
-
-
?
UTP + deoxyadenosine
UDP + dAMP
22% activity compared to ATP
-
-
?
additional information
?
-
-
less than 1% activity with dGTP, deoxyuridine, deoxythymidine, 1-beta-D-arabinofuranosyl uracil, 2',3'-dideoxyguanosine, 2',3'-dideoxycytidine, cytidine, acyclovir, ganciclovir, 2'-deoxy-2'-fluoro-1-beta-D-arabinofuranosyl-5-iodouracil, and 5-fluoro-2'-deoxyuridine
-
-
?
additional information
?
-
less than 1% activity with dGTP, deoxyuridine, deoxythymidine, 1-beta-D-arabinofuranosyl uracil, 2',3'-dideoxyguanosine, 2',3'-dideoxycytidine, cytidine, acyclovir, ganciclovir, 2'-deoxy-2'-fluoro-1-beta-D-arabinofuranosyl-5-iodouracil, and 5-fluoro-2'-deoxyuridine
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.016
ATP
-
with deoxyadenosine as phosphoryl acceptor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
0.021
CTP
-
with deoxyadenosine as phosphoryl acceptor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
0.01
deoxyadenosine
-
with ATP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
0.018
deoxyadenosine
-
with CTP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
0.121
deoxycytidine
-
with ATP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
0.146
deoxycytidine
-
with CTP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
0.019
deoxyguanosine
-
with CTP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
0.02
deoxyguanosine
-
with ATP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
0.077
TTP
-
with deoxyadenosine as phosphoryl acceptor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
0.135
UTP
-
with deoxyadenosine as phosphoryl acceptor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
50000
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dimer
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
in complex with deoxyadenosine 5'-triphosphate and 2'-deoxycytidine 5'-triphosphate, as well as with products 2'-deoxycytidine 5'-phosphate and 2'-deoxycytidine 5'-diphosphate. Both deoxyadenosine 5'-triphosphate and 2'-deoxycytidine 5'-triphosphate bind to the enzyme in a feedback-inhibitory manner with the deoxyribonucleoside part in the deoxyribonucleoside binding site and the triphosphates in the P-loop. Superposition with human counterparts deoxyguanosine kinase and deoxycytidine kinase. Overall structures are very similar with a few amino acid alterations in the proximity of the active site
sitting drop vapour diffusion method using 24% (w/v) PEG 3350 and 0.2 M KSCN
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Wang, L.; Westberg, J.; Bolske, G.; Eriksson, S.
Novel deoxynucleoside-phosphorylating enzymes in mycoplasmas: evidence for efficient utilization of deoxynucleosides
Mol. Microbiol.
42
1065-1073
2001
Acholeplasma laidlawii, Mycoplasmopsis arginini, Mycoplasma mycoides, Mycoplasma pneumoniae
Pol, E.; Wang, L.
Kinetic mechanism of deoxyadenosine kinase from Mycoplasma determined by surface plasmon resonance technology
Biochemistry
45
513-522
2006
Mycoplasma mycoides
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