BRENDA - Enzyme Database show
show all sequences of 2.7.1.76

Novel deoxynucleoside-phosphorylating enzymes in mycoplasmas: evidence for efficient utilization of deoxynucleosides

Wang, L.; Westberg, J.; Bolske, G.; Eriksson, S.; Mol. Microbiol. 42, 1065-1073 (2001)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Mycoplasma mycoides
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.011
-
ATP
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
0.012
-
deoxyadenosine
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
0.021
-
deoxyguanosine
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
0.022
-
CTP
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
0.063
-
TTP
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
0.18
-
deoxycytidine
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
0.18
-
UTP
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27000
-
2 * 27000, His-tagged enzyme, SDS-PAGE
Mycoplasma mycoides
50000
-
gel filtration
Mycoplasma mycoides
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acholeplasma laidlawii
-
-
-
Mycoplasma arginini
-
-
-
Mycoplasma mycoides
-
subspecies mycoides SC
-
Mycoplasma pneumoniae
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant His-tagged enzyme, metal affinity chromatography
Mycoplasma mycoides
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + deoxyadenosine
-
641988
Acholeplasma laidlawii
ADP + dAMP
-
641988
Acholeplasma laidlawii
?
ATP + deoxyadenosine
-
641988
Mycoplasma mycoides
ADP + dAMP
-
641988
Mycoplasma mycoides
?
ATP + deoxyadenosine
-
641988
Mycoplasma pneumoniae
ADP + dAMP
-
641988
Mycoplasma pneumoniae
?
ATP + deoxyadenosine
-
641988
Mycoplasma arginini
ADP + dAMP
-
641988
Mycoplasma arginini
?
ATP + deoxycytidine
-
641988
Mycoplasma mycoides
ADP + dCMP
-
641988
Mycoplasma mycoides
?
ATP + deoxyguanosine
approx. 40% of activity with deoxyadenosine
641988
Mycoplasma mycoides
ADP + dGMP
-
641988
Mycoplasma mycoides
?
CTP + deoxyadenosine
-
641988
Mycoplasma mycoides
CDP + dAMP
-
641988
Mycoplasma mycoides
?
GTP + deoxyadenosine
10% of activity with ATP
641988
Mycoplasma mycoides
GDP + dAMP
-
641988
Mycoplasma mycoides
?
TTP + deoxyadenosine
-
641988
Mycoplasma mycoides
TDP + dAMP
-
641988
Mycoplasma mycoides
?
UTP + deoxyadenosine
-
641988
Mycoplasma mycoides
UDP + dAMP
-
641988
Mycoplasma mycoides
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 27000, His-tagged enzyme, SDS-PAGE
Mycoplasma mycoides
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.36
-
UTP
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
0.61
-
deoxyguanosine
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
0.62
-
CTP
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
0.84
-
ATP
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
1.23
-
deoxycytidine
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
1.48
-
deoxyadenosine
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
1.64
-
TTP
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Mycoplasma mycoides
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.011
-
ATP
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
0.012
-
deoxyadenosine
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
0.021
-
deoxyguanosine
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
0.022
-
CTP
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
0.063
-
TTP
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
0.18
-
deoxycytidine
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
0.18
-
UTP
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27000
-
2 * 27000, His-tagged enzyme, SDS-PAGE
Mycoplasma mycoides
50000
-
gel filtration
Mycoplasma mycoides
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged enzyme, metal affinity chromatography
Mycoplasma mycoides
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + deoxyadenosine
-
641988
Acholeplasma laidlawii
ADP + dAMP
-
641988
Acholeplasma laidlawii
?
ATP + deoxyadenosine
-
641988
Mycoplasma mycoides
ADP + dAMP
-
641988
Mycoplasma mycoides
?
ATP + deoxyadenosine
-
641988
Mycoplasma pneumoniae
ADP + dAMP
-
641988
Mycoplasma pneumoniae
?
ATP + deoxyadenosine
-
641988
Mycoplasma arginini
ADP + dAMP
-
641988
Mycoplasma arginini
?
ATP + deoxycytidine
-
641988
Mycoplasma mycoides
ADP + dCMP
-
641988
Mycoplasma mycoides
?
ATP + deoxyguanosine
approx. 40% of activity with deoxyadenosine
641988
Mycoplasma mycoides
ADP + dGMP
-
641988
Mycoplasma mycoides
?
CTP + deoxyadenosine
-
641988
Mycoplasma mycoides
CDP + dAMP
-
641988
Mycoplasma mycoides
?
GTP + deoxyadenosine
10% of activity with ATP
641988
Mycoplasma mycoides
GDP + dAMP
-
641988
Mycoplasma mycoides
?
TTP + deoxyadenosine
-
641988
Mycoplasma mycoides
TDP + dAMP
-
641988
Mycoplasma mycoides
?
UTP + deoxyadenosine
-
641988
Mycoplasma mycoides
UDP + dAMP
-
641988
Mycoplasma mycoides
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 27000, His-tagged enzyme, SDS-PAGE
Mycoplasma mycoides
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.36
-
UTP
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
0.61
-
deoxyguanosine
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
0.62
-
CTP
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
0.84
-
ATP
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
1.23
-
deoxycytidine
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
1.48
-
deoxyadenosine
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
1.64
-
TTP
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
Other publictions for EC 2.7.1.76
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739204
Clausen
Plants salvage deoxyribonucleo ...
Arabidopsis thaliana, Solanum tuberosum
Nucleosides Nucleotides Nucleic Acids
33
291-295
2014
-
-
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-
-
-
-
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2
-
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2
-
6
-
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1
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2
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2
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2
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1
-
-
2
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
694483
Joachims
Inhibition of deoxynucleoside ...
Homo sapiens
Nucleosides Nucleotides Nucleic Acids
27
816-820
2008
-
1
-
-
-
-
1
-
-
-
-
-
-
1
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-
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1
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1
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1
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1
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-
-
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-
-
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674219
Sandrini
Nucleoside analogues are activ ...
Bacillus cereus
J. Antimicrob. Chemother.
60
510-520
2007
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-
1
-
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-
-
6
-
-
1
-
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11
-
-
1
-
-
-
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6
-
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3
-
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-
1
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-
1
1
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6
-
-
1
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1
-
-
-
-
6
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
675428
Welin
Structure-function analysis of ...
Mycoplasma mycoides
J. Mol. Biol.
366
1615-1623
2007
-
-
-
1
-
-
2
-
-
-
2
-
-
5
-
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1
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46
1
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1
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1
2
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2
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2
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1
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-
46
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
675444
Sandrini
Dictyostelium discoideum salva ...
Dictyostelium discoideum
J. Mol. Biol.
369
653-664
2007
-
-
1
1
-
-
-
5
-
-
1
1
-
7
-
-
-
-
-
-
-
-
10
1
-
-
-
6
-
-
-
1
-
-
-
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1
1
1
-
-
-
-
-
6
-
-
1
1
-
-
-
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-
-
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10
1
-
-
-
6
-
-
-
-
-
-
-
-
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-
690459
Sandrini
Deoxyribonucleoside kinases ac ...
Staphylococcus aureus
Antimicrob. Agents Chemother.
51
2726-2732
2007
-
-
1
-
-
-
-
3
-
-
1
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7
-
-
-
-
-
-
-
-
3
1
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-
-
3
-
-
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-
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-
1
-
-
-
-
-
-
-
3
-
-
1
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-
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-
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3
1
-
-
-
3
-
-
-
-
-
-
-
-
-
-
672159
Pol
Kinetic mechanism of deoxyaden ...
Mycoplasma mycoides
Biochemistry
45
513-522
2006
-
-
-
-
-
-
-
10
-
-
-
-
-
3
-
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6
-
-
-
-
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-
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-
1
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-
1
-
-
-
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-
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10
-
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-
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-
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6
-
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-
-
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-
-
-
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-
-
-
676722
Katahira
Profiles of purine biosynthesi ...
Solanum tuberosum
Planta
225
115-126
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
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-
-
1
1
-
1
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1
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1
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1
1
-
1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
660855
Krawiec
Inorganic tripolyphosphate (PP ...
Homo sapiens
Biochem. Biophys. Res. Commun.
301
192-197
2003
-
-
-
-
-
-
1
4
-
1
-
2
-
1
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-
-
2
-
4
-
1
-
-
-
1
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-
2
2
-
-
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-
-
2
-
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-
1
2
4
-
1
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2
-
-
-
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-
2
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
662985
Krawiec
Ability of adenosine-2'(3')-de ...
Homo sapiens
Nucleosides Nucleotides Nucleic Acids
22
1525-1529
2003
-
-
-
-
-
-
-
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1
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2
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1
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2
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14
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7
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7
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1
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2
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2
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14
-
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640561
Andersen
Deoxynucleoside kinases encode ...
Bacillus subtilis
J. Biol. Chem.
276
5518-5524
2001
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1
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1
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3
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1
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1
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1
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1
1
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1
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1
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3
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1
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1
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-
641988
Wang
Novel deoxynucleoside-phosphor ...
Acholeplasma laidlawii, Mycoplasma arginini, Mycoplasma mycoides, Mycoplasma pneumoniae
Mol. Microbiol.
42
1065-1073
2001
-
-
1
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7
-
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2
-
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12
-
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1
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10
1
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7
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1
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7
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2
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1
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10
1
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7
-
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641989
Park
-
Protein engineering of deoxynu ...
Lactobacillus acidophilus, Lactobacillus acidophilus ATCC 700396
J. Food Sci. Nutr.
6
79-81
2001
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3
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4
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641984
Jenuth
Modeling of purine nucleoside ...
Mus musculus
Biochem. Cell Biol.
74
219-225
1996
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1
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1
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1
1
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2
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641987
Szabados
Metabolism of adenosine and de ...
Homo sapiens
Int. J. Biochem. Cell Biol.
28
1405-1414
1996
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2
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1
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2
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2
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640565
Hong
Directed mutagenesis of deoxyg ...
Lactobacillus acidophilus, Lactobacillus acidophilus ATCC 700396
J. Biol. Chem.
270
6602-6606
1995
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-
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4
-
1
5
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6
-
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2
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4
-
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1
2
5
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640567
Ma
Cloning and expression of the ...
Lactobacillus acidophilus, Lactobacillus acidophilus ATCC 700396
J. Biol. Chem.
270
6595-6601
1995
1
-
1
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1
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2
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4
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1
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4
1
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1
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4
1
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640566
Ikeda
Heterodimeric deoxynucleoside ...
Lactobacillus acidophilus, Lactobacillus acidophilus ATCC 700396
Biochemistry
33
5328-5334
1994
-
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1
4
-
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2
-
-
2
-
-
1
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-
1
2
2
1
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