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Literature summary for 2.7.1.76 extracted from

  • Wang, L.; Westberg, J.; Bolske, G.; Eriksson, S.
    Novel deoxynucleoside-phosphorylating enzymes in mycoplasmas: evidence for efficient utilization of deoxynucleosides (2001), Mol. Microbiol., 42, 1065-1073.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mycoplasma mycoides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
ATP pH 7.6, 37°C, recombinant enzyme Mycoplasma mycoides
0.012
-
deoxyadenosine pH 7.6, 37°C, recombinant enzyme Mycoplasma mycoides
0.021
-
deoxyguanosine pH 7.6, 37°C, recombinant enzyme Mycoplasma mycoides
0.022
-
CTP pH 7.6, 37°C, recombinant enzyme Mycoplasma mycoides
0.063
-
TTP pH 7.6, 37°C, recombinant enzyme Mycoplasma mycoides
0.18
-
UTP pH 7.6, 37°C, recombinant enzyme Mycoplasma mycoides
0.18
-
deoxycytidine pH 7.6, 37°C, recombinant enzyme Mycoplasma mycoides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
2 * 27000, His-tagged enzyme, SDS-PAGE Mycoplasma mycoides
50000
-
gel filtration Mycoplasma mycoides

Organism

Organism UniProt Comment Textmining
Acholeplasma laidlawii
-
-
-
Mycoplasma mycoides
-
subspecies mycoides SC
-
Mycoplasma pneumoniae
-
-
-
Mycoplasmopsis arginini
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme, metal affinity chromatography Mycoplasma mycoides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + deoxyadenosine
-
Acholeplasma laidlawii ADP + dAMP
-
?
ATP + deoxyadenosine
-
Mycoplasma mycoides ADP + dAMP
-
?
ATP + deoxyadenosine
-
Mycoplasma pneumoniae ADP + dAMP
-
?
ATP + deoxyadenosine
-
Mycoplasmopsis arginini ADP + dAMP
-
?
ATP + deoxycytidine
-
Mycoplasma mycoides ADP + dCMP
-
?
ATP + deoxyguanosine approx. 40% of activity with deoxyadenosine Mycoplasma mycoides ADP + dGMP
-
?
CTP + deoxyadenosine
-
Mycoplasma mycoides CDP + dAMP
-
?
GTP + deoxyadenosine 10% of activity with ATP Mycoplasma mycoides GDP + dAMP
-
?
TTP + deoxyadenosine
-
Mycoplasma mycoides TDP + dAMP
-
?
UTP + deoxyadenosine
-
Mycoplasma mycoides UDP + dAMP
-
?

Subunits

Subunits Comment Organism
dimer 2 * 27000, His-tagged enzyme, SDS-PAGE Mycoplasma mycoides

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.36
-
UTP pH 7.6, 37°C, recombinant enzyme Mycoplasma mycoides
0.61
-
deoxyguanosine pH 7.6, 37°C, recombinant enzyme Mycoplasma mycoides
0.62
-
CTP pH 7.6, 37°C, recombinant enzyme Mycoplasma mycoides
0.84
-
ATP pH 7.6, 37°C, recombinant enzyme Mycoplasma mycoides
1.23
-
deoxycytidine pH 7.6, 37°C, recombinant enzyme Mycoplasma mycoides
1.48
-
deoxyadenosine pH 7.6, 37°C, recombinant enzyme Mycoplasma mycoides
1.64
-
TTP pH 7.6, 37°C, recombinant enzyme Mycoplasma mycoides