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Information on EC 2.7.1.199 - protein-Npi-phosphohistidine-D-glucose phosphotransferase and Organism(s) Corynebacterium glutamicum and UniProt Accession Q46072

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IUBMB Comments
This enzyme is a component (known as enzyme II) of a phosphoenolpyruvate (PEP)-dependent, sugar transporting phosphotransferase system (PTS). The system, which is found only in prokaryotes, simultaneously transports its substrate from the periplasm or extracellular space into the cytoplasm and phosphorylates it. The phosphate donor, which is shared among the different systems, is a phospho-carrier protein of low molecular mass that has been phosphorylated by EC 2.7.3.9 (phosphoenolpyruvate---protein phosphotransferase). Enzyme II, on the other hand, is specific for a particular substrate, although in some cases alternative substrates can be transported with lower efficiency. The reaction involves a successive transfer of the phosphate group to several amino acids within the enzyme before the final transfer to the substrate.
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Corynebacterium glutamicum
UNIPROT: Q46072
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The taxonomic range for the selected organisms is: Corynebacterium glutamicum
The enzyme appears in selected viruses and cellular organisms
Synonyms
glucose transporter, iiaglc, glucose permease, iiglc, d-glucose transporter, enzyme iiglc, eiiglc, eiiaglc, iicbglc, sugar permease, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glucose-specific PTS enzyme II
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D-glucose PTS permease
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-
-
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EIIBCA
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catalytic subunit
EIIGlc
glucose specific transporter
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glucose-specific enzyme II
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glucose-specific phosphotransferase system
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phosphoenolpyruvate-dependent glucose phosphotransferase system
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SYSTEMATIC NAME
IUBMB Comments
protein-Npi-phospho-L-histidine:D-glucose Npi-phosphotransferase
This enzyme is a component (known as enzyme II) of a phosphoenolpyruvate (PEP)-dependent, sugar transporting phosphotransferase system (PTS). The system, which is found only in prokaryotes, simultaneously transports its substrate from the periplasm or extracellular space into the cytoplasm and phosphorylates it. The phosphate donor, which is shared among the different systems, is a phospho-carrier protein of low molecular mass that has been phosphorylated by EC 2.7.3.9 (phosphoenolpyruvate---protein phosphotransferase). Enzyme II, on the other hand, is specific for a particular substrate, although in some cases alternative substrates can be transported with lower efficiency. The reaction involves a successive transfer of the phosphate group to several amino acids within the enzyme before the final transfer to the substrate.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
[protein]-Npi-phospho-L-histidine + D-glucose[side 1]
[protein]-L-histidine + D-glucose 6-phosphate[side 2]
show the reaction diagram
-
-
-
?
[protein]-Npi-phospho-L-histidine + D-glucose[side 1]
[protein]-L-histidine + D-glucose 6-phosphate[side 2]
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
[protein]-Npi-phospho-L-histidine + D-glucose[side 1]
[protein]-L-histidine + D-glucose 6-phosphate[side 2]
show the reaction diagram
-
-
-
?
[protein]-Npi-phospho-L-histidine + D-glucose[side 1]
[protein]-L-histidine + D-glucose 6-phosphate[side 2]
show the reaction diagram
-
-
-
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
-
the phosphoenolpyruvate-dependent glucose phosphotransferase system is the major uptake system responsible for transporting glucose, and is involved in glucose translocation and phosphorylation in Corynebacterium glutamicum
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
71543
-
x * 71543, calculated from amino acid sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 71543, calculated from amino acid sequence
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli ZSC113 cells
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
enzyme expression is repressed by the DeoR-type regulator SugR, RamB, and GlxR
the transcriptional regulators GntR1 and GntR2 activate the enzyme expression
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Subhadra, B.; Lee, J.K.
Elucidation of the regulation of ethanol catabolic genes and ptsG using a glxR and adenylate cyclase gene (cyaB) deletion mutants of Corynebacterium glutamicum ATCC 13032
J. Microbiol. Biotechnol.
23
1683-1690
2013
Corynebacterium glutamicum (Q46072), Corynebacterium glutamicum, Corynebacterium glutamicum ATCC 13032 (Q46072), Corynebacterium glutamicum ATCC 13032
Manually annotated by BRENDA team
Yoon, K.; Lee, K.; Lee, J.; Park, S.
Cloning, nucleotide sequencing, and characterization of the ptsG gene encoding glucose-specific enzyme II of the phosphotransferase system from Brevibacterium lactofermentum
J. Microbiol. Biotechnol.
9
582-588
1999
Corynebacterium glutamicum, Corynebacterium glutamicum ATCC 13869
-
Manually annotated by BRENDA team
Xu, J.; Zhang, J.; Liu, D.; Zhang, W.
Increased glucose utilization and cell growth of Corynebacterium glutamicum by modifying the glucose-specific phosphotransferase system (PTSGlc) genes
Can. J. Microbiol.
62
983-992
2016
Corynebacterium glutamicum, Corynebacterium glutamicum XQ-8
Manually annotated by BRENDA team
Martins, G.; Giacomelli, G.; Goldbeck, O.; Seibold, G.; Bramkamp, M.
Substrate-dependent cluster density dynamics of Corynebacterium glutamicum phosphotransferase system permeases
Mol. Microbiol.
111
1335-1354
2019
Corynebacterium glutamicum, Corynebacterium glutamicum RES 167
Manually annotated by BRENDA team
Ruan, H.; Yu, H.; Xu, J.
The glucose uptake systems in Corynebacterium glutamicum a review
World J. Microbiol. Biotechnol.
36
126
2020
Corynebacterium glutamicum
Manually annotated by BRENDA team