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Information on EC 2.7.1.199 - protein-Npi-phosphohistidine-D-glucose phosphotransferase and Organism(s) Escherichia coli and UniProt Accession P69786

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IUBMB Comments
This enzyme is a component (known as enzyme II) of a phosphoenolpyruvate (PEP)-dependent, sugar transporting phosphotransferase system (PTS). The system, which is found only in prokaryotes, simultaneously transports its substrate from the periplasm or extracellular space into the cytoplasm and phosphorylates it. The phosphate donor, which is shared among the different systems, is a phospho-carrier protein of low molecular mass that has been phosphorylated by EC 2.7.3.9 (phosphoenolpyruvate---protein phosphotransferase). Enzyme II, on the other hand, is specific for a particular substrate, although in some cases alternative substrates can be transported with lower efficiency. The reaction involves a successive transfer of the phosphate group to several amino acids within the enzyme before the final transfer to the substrate.
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Escherichia coli
UNIPROT: P69786
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The taxonomic range for the selected organisms is: Escherichia coli
The enzyme appears in selected viruses and cellular organisms
Synonyms
glucose transporter, iiaglc, glucose permease, iiglc, d-glucose transporter, enzyme iiglc, eiiglc, eiiaglc, iicbglc, sugar permease, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glucose transporter
-
glucose-permease
-
D-glucose PTS permease
-
-
-
-
D-glucose transporter
-
-
EIIAGlc
EIIGlc
enzyme IICBGlc
-
-
enzyme IIGlc
-
-
glucose transporter
-
-
glucose-PTS transporter
-
-
glucose-specific carrier
-
-
glucose-specific EII complex
-
glucose-specific permease
-
-
glucose-specific PTS permease
-
-
glucose-specific transporter of phosphoenolpyruvate:carbohydrate phosphotransferase system
-
-
glucose-specific transporter of the phosphotransferase system
-
-
high affinity Glc transporter
-
-
IIBCGlc
-
-
IICB
the glucose-specific phosphotransferase system includes the integral membrane protein IICB that couples the transmembrane transport of D-glucose to its phosphorylation
IICBGlc
IICglc
glucose-specific IIC transport domain
non-PTS sugar transporter
-
-
phosphoenolpyruvate-dependent glucose-phosphotransferase system
-
-
PTS IIC glucose transporter
-
ptsG-I
-
isoform
SYSTEMATIC NAME
IUBMB Comments
protein-Npi-phospho-L-histidine:D-glucose Npi-phosphotransferase
This enzyme is a component (known as enzyme II) of a phosphoenolpyruvate (PEP)-dependent, sugar transporting phosphotransferase system (PTS). The system, which is found only in prokaryotes, simultaneously transports its substrate from the periplasm or extracellular space into the cytoplasm and phosphorylates it. The phosphate donor, which is shared among the different systems, is a phospho-carrier protein of low molecular mass that has been phosphorylated by EC 2.7.3.9 (phosphoenolpyruvate---protein phosphotransferase). Enzyme II, on the other hand, is specific for a particular substrate, although in some cases alternative substrates can be transported with lower efficiency. The reaction involves a successive transfer of the phosphate group to several amino acids within the enzyme before the final transfer to the substrate.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
[protein]-Npi-phospho-L-histidine + D-glucose[side 1]
[protein]-L-histidine + D-glucose 6-phosphate[side 2]
show the reaction diagram
[protein]-Npi-phospho-L-histidine + 2',3'-epoxypropyl beta-D-glucopyranoside
?
show the reaction diagram
-
pseudosubstrate
-
-
?
[protein]-Npi-phospho-L-histidine + chloroacetyl beta-D-glucopyranoside
?
show the reaction diagram
-
pseudosubstrate
-
-
?
[protein]-Npi-phospho-L-histidine + D-glucose[side 1]
[protein]-L-histidine + D-glucose 6-phosphate[side 2]
show the reaction diagram
[protein]-Npi-phospho-L-histidine + methyl alpha-D-glucopyranoside
[protein]-L-histidine + methyl alpha-D-glucopyranoside 6-phosphate
show the reaction diagram
-
-
-
-
?
[protein]-Npi-phospho-L-histidine + methyl-alpha-D-glucopyranoside
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
[protein]-Npi-phospho-L-histidine + D-glucose[side 1]
[protein]-L-histidine + D-glucose 6-phosphate[side 2]
show the reaction diagram
[protein]-Npi-phospho-L-histidine + D-glucose[side 1]
[protein]-L-histidine + D-glucose 6-phosphate[side 2]
show the reaction diagram
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
SgrT
allosteric inhibitor
-
2',3'-epoxypropyl beta-D-glucopyranoside
-
-
beta-D-glucopyranosyl isothiocyanate
-
-
beta-D-glucopyranosyl phenyl isothiocyanate
-
-
chloroacetyl beta-D-glucopyranoside
-
-
isopropyl-beta-D-thiogalactopyranoside
-
inhibitory above 0.075 mM
methyl 6,7-anhydro-DL-glycero-alpha-D-gluco-heptopyranoside
-
-
methyl 6-deoxy-6-isothiocyanate-alpha-D-glucopyranoside
-
-
methyl alpha-D-glucopyranoside
-
-
methyl alpha-D-mannopyranoside
-
-
additional information
-
not inhibited by beta-D-glucopyranosyl isothiocyanate, methyl 6-O-chloroacetyl-alpha-D-glucopyranoside, and (6E)-6,7-dideoxy-alpha-D-gluco-oct-6-enopyranosiduronic acid
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
D-glucose
D-glucose strongly induces enzyme activity in the wild type
D-glucose
-
the wild type enzyme activity is induced about 3fold by D-glucose
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.028
2',3'-epoxypropyl beta-D-glucopyranoside
-
at pH 7.5 and 30°C
0.7
chloroacetyl beta-D-glucopyranoside
-
at pH 7.5 and 30°C
0.06
D-glucose[side 1]
-
at pH 7.5 and 30°C
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.001
2',3'-epoxypropyl beta-D-glucopyranoside
Escherichia coli
-
at pH 7.5 and 30°C
0.0024
beta-D-glucopyranosyl phenyl isothiocyanate
Escherichia coli
-
at pH 7.5 and 30°C
0.0013
chloroacetyl beta-D-glucopyranoside
Escherichia coli
-
at pH 7.5 and 30°C
0.00007
methyl 6,7-anhydro-DL-glycero-alpha-D-gluco-heptopyranoside
Escherichia coli
-
at pH 7.5 and 30°C
0.0009
methyl 6-deoxy-6-isothiocyanate-alpha-D-glucopyranoside
Escherichia coli
-
at pH 7.5 and 30°C
0.0008
methyl alpha-D-glucopyranoside
Escherichia coli
-
at pH 7.5 and 30°C
0.0008
methyl alpha-D-mannopyranoside
Escherichia coli
-
at pH 7.5 and 30°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
106000
analytical ultracentrifugation
50645
2 * 50645, calculated from amino acid sequence
9600
-
x * 9600, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
homodimer
x-ray crystallography
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
the enzyme is phosphorylated at C421 of the B-domain
phosphoprotein
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C421S
inactive
F37Y
-
the mutant shows reduced activity with D-glucose and therefore increased activity towards D-ribose
G176D
-
the mutant shows reduced activity with D-glucose and therefore increased activity towards D-ribose
G281D
-
the mutant shows reduced activity with D-glucose and therefore increased activity towards D-ribose
I283T
-
the mutant shows reduced activity with D-glucose and therefore increased activity towards D-ribose
L289Q
-
the mutant shows reduced activity with D-glucose and therefore increased activity towards D-ribose
V12F
-
fructose-utilizing mutant
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA agarose column chromatography and Superose 12 gel filtration
Ni-NTA agarose column chromatography
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli strain W3110 and ZSC112DELTAG
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression of the major ptsG transcript is induced by repressed by the Mlc protein
-
in the absence of glucose, ptsG expression is repressed by the repressor Mlc, while in the presence of glucose Mlc is inactivated by binding to the dephosphorylated EIIBGlc domain of the EIICBGlc during glucose transport
-
phospho-ArcA represses ptsG P1 transcription by decreasing the cAMP receptor protein binding
-
protein YeeI is involved in the regulation of gene expression by inactivating repressor Ml. The enzyme synthesis is increased 6.2fold after addition of 0.2% (w/v) D-glucose
-
the enzyme expression is higher in D-glucose media than in glycerol. A mutation in the mlc gene greatly enhances enzyme expression in a glycerol-grown culture but has no effect on enzyme expression during growth on D-glucose
-
the umgC mutation (which allows a strain mutated in the mannose phosphotransferase system to grow on glucosamine) enhances enzyme mRNA levels
-
transcription of ptsG is regulated by the repressor Mlc
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Gabor, E.; Goehler, A.K.; Kosfeld, A.; Staab, A.; Kremling, A.; Jahreis, K.
The phosphoenolpyruvate-dependent glucose-phosphotransferase system from Escherichia coli K-12 as the center of a network regulating carbohydrate flux in the cell
Eur. J. Cell Biol.
90
711-720
2011
Escherichia coli
Manually annotated by BRENDA team
Chatterjee, R.; Millard, C.S.; Champion, K.; Clark, D.P.; Donnelly, M.I.
Mutation of the ptsG gene results in increased production of succinate in fermentation of glucose by Escherichia coli
Appl. Environ. Microbiol.
67
148-154
2001
Escherichia coli, Escherichia coli NZN111
Manually annotated by BRENDA team
Garcia-Alles, L.F.; Navdaeva, V.; Haenni, S.; Erni, B.
The glucose-specific carrier of the Escherichia coli phosphotransferase system
Eur. J. Biochem.
269
4969-4980
2002
Escherichia coli
Manually annotated by BRENDA team
Ruyter, G.J.; Postma, P.W.; van Dam, K.
Control of glucose metabolism by enzyme IIGlc of the phosphoenolpyruvate-dependent phosphotransferase system in Escherichia coli
J. Bacteriol.
173
6184-6191
1991
Escherichia coli
Manually annotated by BRENDA team
Zeppenfeld, T.; Larisch, C.; Lengeler, J.W.; Jahreis, K.
Glucose transporter mutants of Escherichia coli K-12 with changes in substrate recognition of IICBGlc and induction behavior of the ptsG gene
J. Bacteriol.
182
4443-4452
2000
Escherichia coli
Manually annotated by BRENDA team
Becker, A.K.; Zeppenfeld, T.; Staab, A.; Seitz, S.; Boos, W.; Morita, T.; Aiba, H.; Mahr, K.; Titgemeyer, F.; Jahreis, K.
YeeI, a novel protein involved in modulation of the activity of the glucose-phosphotransferase system in Escherichia coli K-12
J. Bacteriol.
188
5439-5449
2006
Escherichia coli
Manually annotated by BRENDA team
Goehler, A.K.; Staab, A.; Gabor, E.; Homann, K.; Klang, E.; Kosfeld, A.; Muus, J.E.; Wulftange, J.S.; Jahreis, K.
Characterization of MtfA, a novel regulatory output signal protein of the glucose-phosphotransferase system in Escherichia coli K-12
J. Bacteriol.
194
1024-1035
2012
Escherichia coli
Manually annotated by BRENDA team
Erni, B.; Zanolari, B.
Glucose-permease of the bacterial phosphotransferase system. Gene cloning, overproduction, and amino acid sequence of enzyme IIGlc
J. Biol. Chem.
261
16398-16403
1986
Escherichia coli (P69786)
Manually annotated by BRENDA team
Buhr, A.; Fluekiger, K.; Erni, B.
The glucose transporter of Escherichia coli. Overexpression, purification, and characterization of functional domains
J. Biol. Chem.
269
23437-23443
1994
Escherichia coli (P69786), Escherichia coli
Manually annotated by BRENDA team
Oh, H.; Park, Y.; Park, C.
A mutated PtsG, the glucose transporter, allows uptake of D-ribose
J. Biol. Chem.
274
14006-14011
1999
Escherichia coli
Manually annotated by BRENDA team
Jeong, J.Y.; Kim, Y.J.; Cho, N.; Shin, D.; Nam, T.W.; Ryu, S.; Seok, Y.J.
Expression of ptsG encoding the major glucose transporter is regulated by ArcA in Escherichia coli
J. Biol. Chem.
279
38513-38518
2004
Escherichia coli
Manually annotated by BRENDA team
Plumbridge, J.
A mutation which affects both the specificity of PtsG sugar transport and the regulation of ptsG expression by Mlc in Escherichia coli
Microbiology
146
2655-2663
2000
Escherichia coli, Escherichia coli JM2053
Manually annotated by BRENDA team
Aboulwafa, M.; Chung, Y.J.; Wai, H.H.; Saier, M.H.
Studies on the Escherichia coli glucose-specific permease, PtsG, with a point mutation in its N-terminal amphipathic leader sequence
Microbiology
149
763-771
2003
Escherichia coli, Escherichia coli HK2240
Manually annotated by BRENDA team
Plumbridge, J.
Expression of ptsG, the gene for the major glucose PTS transporter in Escherichia coli, is repressed by Mlc and induced by growth on glucose
Mol. Microbiol.
29
1053-1063
1998
Escherichia coli
Manually annotated by BRENDA team
Kornberg, H.L.; Lambourne, L.T.; Sproul, A.A.
Facilitated diffusion of fructose via the phosphoenolpyruvate/glucose phosphotransferase system of Escherichia coli
Proc. Natl. Acad. Sci. USA
97
1808-1812
2000
Escherichia coli, Escherichia coli HK 2190
Manually annotated by BRENDA team
Jeckelmann, J.M.; Erni, B.
The mannose phosphotransferase system (Man-PTS) - mannose transporter and receptor for bacteriocins and bacteriophages
Biochim. Biophys. Acta Biomembr.
1862
183412
2020
Escherichia coli
Manually annotated by BRENDA team
Kim, H.J.; Jeong, H.; Lee, S.J.
Short-term adaptation modulates anaerobic metabolic flux to succinate by activating ExuT, a novel D-glucose transporter in Escherichia coli
Front. Microbiol.
11
27
2020
Escherichia coli
Manually annotated by BRENDA team
Kalbermatter, D.; Chiu, P.L.; Jeckelmann, J.M.; Ucurum, Z.; Walz, T.; Fotiadis, D.
Electron crystallography reveals that substrate release from the PTS IIC glucose transporter is coupled to a subtle conformational change
J. Struct. Biol.
199
39-45
2017
Escherichia coli (P69783), Escherichia coli
Manually annotated by BRENDA team
Perez-Morales, D.; Bustamante, V.
The global regulatory system Csr senses glucose through the phosphoenolpyruvate carbohydrate phosphotransferase system
Mol. Microbiol.
99
623-626
2016
Escherichia coli (P69783), Escherichia coli
Manually annotated by BRENDA team
Leng, Y.; Vakulskas, C.; Zere, T.; Pickering, B.; Watnick, P.; Babitzke, P.; Romeo, T.
Regulation of CsrB/C sRNA decay by EIIAGlc of the phosphoenolpyruvate carbohydrate phosphotransferase system
Mol. Microbiol.
99
627-639
2016
Escherichia coli
Manually annotated by BRENDA team
Jeckelmann, J.M.; Erni, B.
Transporters of glucose and other carbohydrates in bacteria
Pflugers Arch.
472
1129-1153
2020
Escherichia coli (P69786)
Manually annotated by BRENDA team