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Information on EC 2.6.1.106 - dTDP-3-amino-3,4,6-trideoxy-alpha-D-glucose transaminase and Organism(s) Streptomyces venezuelae and UniProt Accession Q9ZGH4

for references in articles please use BRENDA:EC2.6.1.106
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EC Tree
IUBMB Comments
A pyridoxal-phosphate protein. The enzyme is involved in the biosynthesis of dTDP-alpha-D-desosamine, a sugar found in several bacterial macrolide antibiotics including erythromycin, megalomicin A, mycinamicin II, and oleandomycin. The reaction occurs in the reverse direction.
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This record set is specific for:
Streptomyces venezuelae
UNIPROT: Q9ZGH4
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The taxonomic range for the selected organisms is: Streptomyces venezuelae
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
dTDP-3-dehydro-4,6-dideoxy-alpha-D-glucopyranose transaminase, DesV, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dTDP-3-dehydro-4,6-dideoxy-alpha-D-glucopyranose transaminase
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SYSTEMATIC NAME
IUBMB Comments
dTDP-3-amino-3,4,6-trideoxy-alpha-D-glucose:2-oxoglutarate aminotransferase
A pyridoxal-phosphate protein. The enzyme is involved in the biosynthesis of dTDP-alpha-D-desosamine, a sugar found in several bacterial macrolide antibiotics including erythromycin, megalomicin A, mycinamicin II, and oleandomycin. The reaction occurs in the reverse direction.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dTDP-3-dehydro-4,6-dideoxy-alpha-D-glucopyranose + L-glutamate
dTDP-3-amino-4,6-dideoxy-alpha-D-glucopyranose + 2-oxoglutarate
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dTDP-3-dehydro-4,6-dideoxy-alpha-D-glucopyranose + L-glutamate
dTDP-3-amino-4,6-dideoxy-alpha-D-glucopyranose + 2-oxoglutarate
show the reaction diagram
the enzyme is involved in biosynthesis of desosamine
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
a pyridoxal-phosphate protein. The pyridoxal 5'-phosphate cofactor is held in place by residues contributed from both subunits (Asp164 and Gln167 from subunit I and Tyr221 and Asn235 from subunit II)
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
DESV_STRVZ
379
0
41184
Swiss-Prot
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
DesV is a homodimer with each subunit containing 12 alpha-helical regions and 12 beta-strands that together form three layers of sheet
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop method of vapor diffusion, three-dimensional structures of both the internal aldimine and the ketimine intermediate with glutamate are determined to 2.05 A resolution
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Burgie, E.S.; Thoden, J.B.; Holden, H.M.
Molecular architecture of DesV from Streptomyces venezuelae: a PLP-dependent transaminase involved in the biosynthesis of the unusual sugar desosamine
Protein Sci.
16
887-896
2007
Streptomyces venezuelae (Q9ZGH4)
Manually annotated by BRENDA team