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Enzyme Nomenclature
Information on EC 2.5.1.28 - dimethylallylcistransferase
for references in articles please use BRENDA:EC2.5.1.28
Word Map on EC 2.5.1.28
- 2.5.1.28
- tomato
- trichomes
- lycopersicum
- solanum
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terpene
- cis-prenyltransferase
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monoterpenes
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glandular
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The enzyme appears in viruses and cellular organisms
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EC NUMBER 
COMMENTARY 
2.5.1.28
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RECOMMENDED NAME
GeneOntology No.
dimethylallylcistransferase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
dimethylallyl diphosphate + isopentenyl diphosphate = diphosphate + neryl diphosphate
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
alkenyl group transfer
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-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
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SYSTEMATIC NAME
IUBMB Comments
dimethylallyl-diphosphate:isopentenyl-diphosphate dimethylallylcistransferase
This enzyme will not use larger prenyl diphosphates as efficient donors.
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CAS REGISTRY NUMBER
COMMENTARY
9032-79-5
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
monoterpene-producing accession LA2409, gene NDPS1 of the CPT1 locus
UniProt
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
evolution
malfunction
physiological function
additional information
homology modeling and three-dimensional structure
evolution
gene SlCPT1 encoding the NDPS1 belongs to the CPT gene family. All the short-chain CPT genes from tomato, i.e. SlCPT1, SlCPT2 and SlCPT6, are closely linked to terpene synthase gene clusters. NDPS1 belongs to the short-chain cis-prenyldiphosphate synthases
evolution
biochemical evolution of terpene biosynthesis in the glandular trichomes of Solanum species, comparative sequence analysis and evolutionary relationship of NDPS1 and (2Z,6Z)-farnesyl diphosphate synthase (EC 2.5.1.92, zFPS), cDNAs from the CPT1 locus, phylogenetic analysis, overview
evolution
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gene SlCPT1 encoding the NDPS1 belongs to the CPT gene family. All the short-chain CPT genes from tomato, i.e. SlCPT1, SlCPT2 and SlCPT6, are closely linked to terpene synthase gene clusters. NDPS1 belongs to the short-chain cis-prenyldiphosphate synthases
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malfunction
RNAi-mediated suppression of NDPS1 leads to a large decrease in beta-phellandrene, which is produced from neryl diphosphate
malfunction
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RNAi-mediated suppression of NDPS1 leads to a large decrease in beta-phellandrene, which is produced from neryl diphosphate
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physiological function
NDPS1 catalyzes the formation of NPP, which is used as a substrate by beta-phellandrene synthase 1, PHS1, to produce beta-phellandrene and several other monoterpenes
physiological function
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NDPS1 catalyzes the formation of NPP, which is used as a substrate by beta-phellandrene synthase 1, PHS1, to produce beta-phellandrene and several other monoterpenes
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
neryl diphosphate + isopentenyl diphosphate
diphosphate + (2Z,6Z)-farnesyl diphosphate
mutant NDPS1-M10
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-
?
additional information
?
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the relative positioning of aromatic amino acid residues at positions 100 and 107 determines the ability of these enzymes to synthesize neryl diphosphate or (2Z,6Z)-farnesyl diphosphate
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dimethylallyl diphosphate + isopentenyl diphosphate
diphosphate + neryl diphosphate
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-
-
?
dimethylallyl diphosphate + isopentenyl diphosphate
diphosphate + neryl diphosphate
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the product neryl diphosphate may be the precursor of cyclic monoterpenes
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-
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dimethylallyl diphosphate + isopentenyl diphosphate
diphosphate + neryl diphosphate
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-
-
?
dimethylallyl diphosphate + isopentenyl diphosphate
diphosphate + neryl diphosphate
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-
-
?
dimethylallyl diphosphate + isopentenyl diphosphate
diphosphate + neryl diphosphate
dimethylallyl diphosphate is the preferred substrate
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-
?
dimethylallyl diphosphate + isopentenyl diphosphate
diphosphate + neryl diphosphate
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-
-
?
dimethylallyl diphosphate + isopentenyl diphosphate
diphosphate + neryl diphosphate
dimethylallyl diphosphate is the preferred substrate
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-
?
dimethylallyl diphosphate + isopentenyl diphosphate
diphosphate + neryl diphosphate
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-
-
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
dimethylallyl diphosphate + isopentenyl diphosphate
diphosphate + neryl diphosphate
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the product neryl diphosphate may be the precursor of cyclic monoterpenes
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-
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dimethylallyl diphosphate + isopentenyl diphosphate
diphosphate + neryl diphosphate
W0J9X7
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-
-
?
dimethylallyl diphosphate + isopentenyl diphosphate
diphosphate + neryl diphosphate
C1K5M2
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-
-
?
dimethylallyl diphosphate + isopentenyl diphosphate
diphosphate + neryl diphosphate
C1K5M2
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-
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?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
additional information
type VI trichomes of leaves and stems, high expression level of SlCPT1
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type VI trichomes of leaves and stems, high expression level of SlCPT1
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additional information
no expression of SlCPT1 in leaves, flowers, roots, and fruits
additional information
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no expression of SlCPT1 in leaves, flowers, roots, and fruits
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene NDPS1 of the CPT1 locus, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, functional expression of codon-optimized synthetic version of CPT1 of accession LA2409, a monoterpene-producing accession, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli
gene SlCPT1, located on chromosome 8, and other gene of the CPT gene family, DNA and amino acid sequence determination and analysis, phylogenetic analysis
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
change of 10 residues that correlate with changes in substrate in NDPS1 from accession LA2409 to those found in zFPS, EC 2.5.1.92, from accession LA1393 (Pro/Ser-45, which corresponds to the second residue in the predicted mature protein, is excluded), and reciprocal changes in zFPS (LA1393). The two resulting recombinant proteins are named NDPS1-M10 and zFPS-M10. The NDPS1-M10 mutant variant is able to utilize dimethylallyl diphosphate and isopentenyl diphosphate to synthesize Z,Z-farnesyl diphosphate at levels that are similar to that of the zFPS enzyme from accession LA1393. The ability of mutant NDPS1-M10 to synthesize Z,Z-farnesyl diphosphate coincides with a greatly reduced ability to synthesize neryl diphosphate
additional information
constitutive and trichome-specific RNAi-mediated knockdown of NDPS1, RNAi-mediated suppression of NDPS1 with greater reductions achieved with the general 35S promoter compared to the trichome-specific MKS1 promoter, phenotypes, overview
additional information
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constitutive and trichome-specific RNAi-mediated knockdown of NDPS1, RNAi-mediated suppression of NDPS1 with greater reductions achieved with the general 35S promoter compared to the trichome-specific MKS1 promoter, phenotypes, overview
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LINKS TO OTHER DATABASES (specific for EC-Number 2.5.1.28)
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