BRENDA - Enzyme Database show
show all sequences of 2.5.1.28

cis-Prenyltransferase atCPT6 produces a family of very short-chain polyisoprenoids in planta

Surmacz, L.; Plochocka, D.; Kania, M.; Danikiewicz, W.; Swiezewska, E.; Biochim. Biophys. Acta 1841, 240-250 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene NDPS1 of the CPT1 locus, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, functional expression of codon-optimized synthetic version of CPT1 of accession LA2409, a monoterpene-producing accession, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli
Solanum habrochaites
Engineering
Amino acid exchange
Commentary
Organism
E98D/Y100S/I106L/I107F
site-directed mutagenesis, mutant M4
Solanum habrochaites
E98D/Y100S/I107F
site-directed mutagenesis, mutant M3V2
Solanum habrochaites
additional information
change of 10 residues that correlate with changes in substrate in NDPS1 from accession LA2409 to those found in zFPS, EC 2.5.1.92, from accession LA1393 (Pro/Ser-45, which corresponds to the second residue in the predicted mature protein, is excluded), and reciprocal changes in zFPS (LA1393). The two resulting recombinant proteins are named NDPS1-M10 and zFPS-M10. The NDPS1-M10 mutant variant is able to utilize dimethylallyl diphosphate and isopentenyl diphosphate to synthesize Z,Z-farnesyl diphosphate at levels that are similar to that of the zFPS enzyme from accession LA1393. The ability of mutant NDPS1-M10 to synthesize Z,Z-farnesyl diphosphate coincides with a greatly reduced ability to synthesize neryl diphosphate
Solanum habrochaites
Y100S/I106L/I107F
site-directed mutagenesis, mutant M3V1
Solanum habrochaites
Y100S/I107F
site-directed mutagenesis, mutant M2
Solanum habrochaites
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.014
-
neryl diphosphate
pH 7.6, 30°C, mutant NDPS1-M10
Solanum habrochaites
0.047
-
dimethylallyl diphosphate
pH 7.6, 30°C, mutant NDPS1-M10
Solanum habrochaites
0.054
-
dimethylallyl diphosphate
pH 7.6, 30°C, mutant NDPS1-M4
Solanum habrochaites
0.097
-
neryl diphosphate
pH 7.6, 30°C, mutant NDPS1-M4
Solanum habrochaites
0.111
-
isopentenyl diphosphate
pH 7.6, 30°C, mutant NDPS1-M4
Solanum habrochaites
0.112
-
dimethylallyl diphosphate
pH 7.6, 30°C, wild-type enzyme
Solanum habrochaites
0.14
-
isopentenyl diphosphate
pH 7.6, 30°C, wild-type enzyme
Solanum habrochaites
0.157
-
isopentenyl diphosphate
pH 7.6, 30°C, mutant NDPS1-M10
Solanum habrochaites
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
-
Solanum habrochaites
9507
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Solanum habrochaites
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dimethylallyl diphosphate + isopentenyl diphosphate
Solanum habrochaites
-
diphosphate + neryl diphosphate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Solanum habrochaites
W0J9X7
monoterpene-producing accession LA2409, gene NDPS1 of the CPT1 locus
-
Purification (Commentary)
Commentary
Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography
Solanum habrochaites
Source Tissue
Source Tissue
Commentary
Organism
Textmining
glandular trichome
type VI glandular trichome
Solanum habrochaites
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dimethylallyl diphosphate + isopentenyl diphosphate
-
737748
Solanum habrochaites
diphosphate + neryl diphosphate
-
-
-
?
additional information
the relative positioning of aromatic amino acid residues at positions 100 and 107 determines the ability of these enzymes to synthesize neryl diphosphate or (2Z,6Z)-farnesyl diphosphate
737748
Solanum habrochaites
?
-
-
-
-
neryl diphosphate + isopentenyl diphosphate
mutant NDPS1-M10
737748
Solanum habrochaites
diphosphate + (2Z,6Z)-farnesyl diphosphate
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Solanum habrochaites
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0064
-
dimethylallyl diphosphate
pH 7.6, 30°C, mutant NDPS1-M10
Solanum habrochaites
0.011
-
isopentenyl diphosphate
pH 7.6, 30°C, mutant NDPS1-M10
Solanum habrochaites
0.026
-
dimethylallyl diphosphate
pH 7.6, 30°C, wild-type enzyme
Solanum habrochaites
0.047
-
isopentenyl diphosphate
pH 7.6, 30°C, wild-type enzyme
Solanum habrochaites
0.088
-
dimethylallyl diphosphate
pH 7.6, 30°C, mutant NDPS1-M4
Solanum habrochaites
0.16
-
isopentenyl diphosphate
pH 7.6, 30°C, mutant NDPS1-M4
Solanum habrochaites
0.18
-
neryl diphosphate
pH 7.6, 30°C, mutant NDPS1-M4
Solanum habrochaites
3.7
-
neryl diphosphate
pH 7.6, 30°C, mutant NDPS1-M10
Solanum habrochaites
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.6
-
assay at
Solanum habrochaites
Cloned(Commentary) (protein specific)
Commentary
Organism
gene NDPS1 of the CPT1 locus, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, functional expression of codon-optimized synthetic version of CPT1 of accession LA2409, a monoterpene-producing accession, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli
Solanum habrochaites
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
E98D/Y100S/I106L/I107F
site-directed mutagenesis, mutant M4
Solanum habrochaites
E98D/Y100S/I107F
site-directed mutagenesis, mutant M3V2
Solanum habrochaites
additional information
change of 10 residues that correlate with changes in substrate in NDPS1 from accession LA2409 to those found in zFPS, EC 2.5.1.92, from accession LA1393 (Pro/Ser-45, which corresponds to the second residue in the predicted mature protein, is excluded), and reciprocal changes in zFPS (LA1393). The two resulting recombinant proteins are named NDPS1-M10 and zFPS-M10. The NDPS1-M10 mutant variant is able to utilize dimethylallyl diphosphate and isopentenyl diphosphate to synthesize Z,Z-farnesyl diphosphate at levels that are similar to that of the zFPS enzyme from accession LA1393. The ability of mutant NDPS1-M10 to synthesize Z,Z-farnesyl diphosphate coincides with a greatly reduced ability to synthesize neryl diphosphate
Solanum habrochaites
Y100S/I106L/I107F
site-directed mutagenesis, mutant M3V1
Solanum habrochaites
Y100S/I107F
site-directed mutagenesis, mutant M2
Solanum habrochaites
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.014
-
neryl diphosphate
pH 7.6, 30°C, mutant NDPS1-M10
Solanum habrochaites
0.047
-
dimethylallyl diphosphate
pH 7.6, 30°C, mutant NDPS1-M10
Solanum habrochaites
0.054
-
dimethylallyl diphosphate
pH 7.6, 30°C, mutant NDPS1-M4
Solanum habrochaites
0.097
-
neryl diphosphate
pH 7.6, 30°C, mutant NDPS1-M4
Solanum habrochaites
0.111
-
isopentenyl diphosphate
pH 7.6, 30°C, mutant NDPS1-M4
Solanum habrochaites
0.112
-
dimethylallyl diphosphate
pH 7.6, 30°C, wild-type enzyme
Solanum habrochaites
0.14
-
isopentenyl diphosphate
pH 7.6, 30°C, wild-type enzyme
Solanum habrochaites
0.157
-
isopentenyl diphosphate
pH 7.6, 30°C, mutant NDPS1-M10
Solanum habrochaites
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
-
Solanum habrochaites
9507
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Solanum habrochaites
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dimethylallyl diphosphate + isopentenyl diphosphate
Solanum habrochaites
-
diphosphate + neryl diphosphate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography
Solanum habrochaites
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
glandular trichome
type VI glandular trichome
Solanum habrochaites
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dimethylallyl diphosphate + isopentenyl diphosphate
-
737748
Solanum habrochaites
diphosphate + neryl diphosphate
-
-
-
?
additional information
the relative positioning of aromatic amino acid residues at positions 100 and 107 determines the ability of these enzymes to synthesize neryl diphosphate or (2Z,6Z)-farnesyl diphosphate
737748
Solanum habrochaites
?
-
-
-
-
neryl diphosphate + isopentenyl diphosphate
mutant NDPS1-M10
737748
Solanum habrochaites
diphosphate + (2Z,6Z)-farnesyl diphosphate
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Solanum habrochaites
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0064
-
dimethylallyl diphosphate
pH 7.6, 30°C, mutant NDPS1-M10
Solanum habrochaites
0.011
-
isopentenyl diphosphate
pH 7.6, 30°C, mutant NDPS1-M10
Solanum habrochaites
0.026
-
dimethylallyl diphosphate
pH 7.6, 30°C, wild-type enzyme
Solanum habrochaites
0.047
-
isopentenyl diphosphate
pH 7.6, 30°C, wild-type enzyme
Solanum habrochaites
0.088
-
dimethylallyl diphosphate
pH 7.6, 30°C, mutant NDPS1-M4
Solanum habrochaites
0.16
-
isopentenyl diphosphate
pH 7.6, 30°C, mutant NDPS1-M4
Solanum habrochaites
0.18
-
neryl diphosphate
pH 7.6, 30°C, mutant NDPS1-M4
Solanum habrochaites
3.7
-
neryl diphosphate
pH 7.6, 30°C, mutant NDPS1-M10
Solanum habrochaites
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.6
-
assay at
Solanum habrochaites
General Information
General Information
Commentary
Organism
evolution
biochemical evolution of terpene biosynthesis in the glandular trichomes of Solanum species, comparative sequence analysis and evolutionary relationship of NDPS1 and (2Z,6Z)-farnesyl diphosphate synthase (EC 2.5.1.92, zFPS), cDNAs from the CPT1 locus, phylogenetic analysis, overview
Solanum habrochaites
additional information
homology modeling and three-dimensional structure
Solanum habrochaites
General Information (protein specific)
General Information
Commentary
Organism
evolution
biochemical evolution of terpene biosynthesis in the glandular trichomes of Solanum species, comparative sequence analysis and evolutionary relationship of NDPS1 and (2Z,6Z)-farnesyl diphosphate synthase (EC 2.5.1.92, zFPS), cDNAs from the CPT1 locus, phylogenetic analysis, overview
Solanum habrochaites
additional information
homology modeling and three-dimensional structure
Solanum habrochaites
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.15
-
dimethylallyl diphosphate
pH 7.6, 30°C, mutant NDPS1-M10
Solanum habrochaites
0.23
-
dimethylallyl diphosphate
pH 7.6, 30°C, wild-type enzyme
Solanum habrochaites
0.28
-
neryl diphosphate
pH 7.6, 30°C, mutant NDPS1-M10
Solanum habrochaites
0.33
-
isopentenyl diphosphate
pH 7.6, 30°C, wild-type enzyme
Solanum habrochaites
0.74
-
isopentenyl diphosphate
pH 7.6, 30°C, mutant NDPS1-M10
Solanum habrochaites
1.6
-
dimethylallyl diphosphate
pH 7.6, 30°C, mutant NDPS1-M4
Solanum habrochaites
1.8
-
isopentenyl diphosphate
pH 7.6, 30°C, mutant NDPS1-M4
Solanum habrochaites
1.8
-
neryl diphosphate
pH 7.6, 30°C, mutant NDPS1-M4
Solanum habrochaites
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.15
-
dimethylallyl diphosphate
pH 7.6, 30°C, mutant NDPS1-M10
Solanum habrochaites
0.23
-
dimethylallyl diphosphate
pH 7.6, 30°C, wild-type enzyme
Solanum habrochaites
0.28
-
neryl diphosphate
pH 7.6, 30°C, mutant NDPS1-M10
Solanum habrochaites
0.33
-
isopentenyl diphosphate
pH 7.6, 30°C, wild-type enzyme
Solanum habrochaites
0.74
-
isopentenyl diphosphate
pH 7.6, 30°C, mutant NDPS1-M10
Solanum habrochaites
1.6
-
dimethylallyl diphosphate
pH 7.6, 30°C, mutant NDPS1-M4
Solanum habrochaites
1.8
-
isopentenyl diphosphate
pH 7.6, 30°C, mutant NDPS1-M4
Solanum habrochaites
1.8
-
neryl diphosphate
pH 7.6, 30°C, mutant NDPS1-M4
Solanum habrochaites
Other publictions for EC 2.5.1.28
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737748
Surmacz
cis-Prenyltransferase atCPT6 p ...
Solanum habrochaites
Biochim. Biophys. Acta
1841
240-250
2014
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1
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5
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8
1
1
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1
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1
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1
-
-
3
-
1
-
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8
1
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1
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5
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8
1
1
-
1
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1
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1
-
-
3
-
1
-
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8
1
-
-
-
-
2
2
-
8
8
723434
Akhtar
The tomato cis-prenyltransfera ...
Solanum lycopersicum, Solanum lycopersicum MP-1
Plant J.
73
640-652
2013
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-
1
-
1
-
-
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1
-
-
2
-
7
-
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-
-
2
-
-
4
-
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1
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1
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1
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2
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2
-
-
4
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
706501
Schilmiller
Monoterpenes in the glandular ...
Solanum lycopersicum
Proc. Natl. Acad. Sci. USA
106
10865-10870
2009
-
-
-
-
-
-
-
2
-
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3
-
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-
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-
3
-
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1
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1
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-
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2
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3
-
-
1
-
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1
-
-
-
-
-
-
-
-
2
2
638431
Banthorpe
-
Biosynthesis of geraniol and n ...
Tanacetum vulgare
Phytochemistry
15
91-100
1976
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-
-
-
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1
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1
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1
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1
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-
-
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-
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-
-
-
-
638430
Beytia
Terpene biosynthesis: formatio ...
Pinus radiata
Arch. Biochem. Biophys.
129
346-356
1969
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1
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1
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1
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1
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1
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1
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1
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2
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