EC Number   |
Protein Variants |
Reference |
|---|
   2.5.1.28 | E98D/Y100S/I106L/I107F |
site-directed mutagenesis, mutant M4 |
737748 |
| 2.5.1.28 | E98D/Y100S/I107F |
site-directed mutagenesis, mutant M3V2 |
737748 |
| 2.5.1.28 | more |
change of 10 residues that correlate with changes in substrate in NDPS1 from accession LA2409 to those found in zFPS, EC 2.5.1.92, from accession LA1393 (Pro/Ser-45, which corresponds to the second residue in the predicted mature protein, is excluded), and reciprocal changes in zFPS (LA1393). The two resulting recombinant proteins are named NDPS1-M10 and zFPS-M10. The NDPS1-M10 mutant variant is able to utilize dimethylallyl diphosphate and isopentenyl diphosphate to synthesize Z,Z-farnesyl diphosphate at levels that are similar to that of the zFPS enzyme from accession LA1393. The ability of mutant NDPS1-M10 to synthesize Z,Z-farnesyl diphosphate coincides with a greatly reduced ability to synthesize neryl diphosphate |
737748 |
| 2.5.1.28 | more |
constitutive and trichome-specific RNAi-mediated knockdown of NDPS1, RNAi-mediated suppression of NDPS1 with greater reductions achieved with the general 35S promoter compared to the trichome-specific MKS1 promoter, phenotypes, overview |
-, 723434 |
| 2.5.1.28 | Y100S/I106L/I107F |
site-directed mutagenesis, mutant M3V1 |
737748 |
| 2.5.1.28 | Y100S/I107F |
site-directed mutagenesis, mutant M2 |
737748 |
