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Synonyms
irx14, irx10, xylan synthase, atcsld5, irx10l, xylan synthetase, ptrgt43b, ptrgt43c, irregular xylem9, beta-1,4-xylan synthase,
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UDP-D-xylose + (1,4-beta-D-xylan)n
UDP + (1,4-beta-D-xylan)n+1
microsomes isolated from transgenic BY2 cells are tested for XylT activity using xylotetraose (Xyl4) as an acceptor and UDP-xylose as a donor. Up to five xylosyl residues with beta-(1,4)-linkages are able to be incorporated by IRX9/IRX14-expressing microsomes
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UDP-D-galacturonic acid + (1,4-beta-D-galacturonic acid)n
UDP + (1,4-beta-D-galacturonic acid)n+1
homogalacturonan synthase activity (substrate: galacturonic acid, GalA) decreased in absence of ATCSLD5 (ATCSLD5-1 mutant line)
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UDP-D-xylose + ((1,4-beta-D-xylooligosaccharide)n)-anthranilic acid
UDP + ((1,4-beta-D-xylooligosaccharide)n+1)-anthranilic acid
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0.1 mM UDP-xylose + 0.5 mM anthranilic acid (AA) labelled xylooligosaccharides (Xyl(2) to Xyl(6)) as acceptor, 200 microgram microsomes, 21°C, pH 6.8, in presence of 5 mM MnCl2, 1 mM dithiothreitol, 0.5% Triton X-100
xylooligosaccharides (up to Xyl(12)) purified by HPLC and analysed by MALDI-TOF mass spectrometry
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UDP-D-xylose + (1,4-beta-D-xylan)n
UDP + (1,4-beta-D-xylan)n+1
UDP-D-xylose + (1,4-beta-D-xylooligosaccharide)n
UDP + (1,4-beta-D-xylooligosaccharide)n+1
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0.1 mM radiolabelled UDP-D-xylose + 0.2 microgram/microlitre (1,4-beta-D-xylopyranose)6, 100 microgram microsomes, 21°C, pH 6.8, in presence of 5 mM MnCl2, 1 mM dithiothreitol, 0.5% Triton X-100, reaction dependent on time and protein concentration
reduction in XylT activity in microsomal fraction of stems from irx9 mutant plants compared to wild-type plants in presence of exogenous substrates can be abolished by complementation with wild-type IRX9 gene, product quantification by scintillation counting
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UDP-D-xylose + (1,4-beta-D-xylopyranose)6
UDP + (1,4-beta-D-xylopyranose)7
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UDP-D-xylose + beta-1,4-xylotetraose
UDP + ?
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UDP-D-xylose + beta-1,4-xylotriose
UDP + ?
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the reaction leads to extension of the beta-1,4-xylotriose by up to five xylose residues, beta-1,4-xylotetraose, beta-1,4-xylopentose, and beta-1,4-xylohexose
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UDP-D-xylose + [(1->4)-beta-D-xylan]n
UDP + [(1->4)-beta-D-xylan]n+1
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additional information
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UDP-D-xylose + (1,4-beta-D-xylan)n
UDP + (1,4-beta-D-xylan)n+1
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precipitation of product by 70% ethanol (final concentration) in presence of wheat arabinoxylan, followed by washing and scintillation counting, control for incorporation into (1,4-beta-D-xylan) by digestion with endo-xylanase
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UDP-D-xylose + (1,4-beta-D-xylan)n
UDP + (1,4-beta-D-xylan)n+1
beta-(1,4)-xylan synthase activity, biosynthesis of xylan (major component of dicot wood)
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UDP-D-xylose + (1,4-beta-D-xylan)n
UDP + (1,4-beta-D-xylan)n+1
microsomes isolated from transgenic BY2 cells are tested for XylT activity using xylotetraose (Xyl4) as an acceptor and UDP-xylose as a donor. Up to five xylosyl residues with beta-(1,4)-linkages are able to be incorporated by IRX9/IRX14-expressing microsomes
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additional information
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xylosyltransferase (XylT) activity, biosynthesis of xylan, a major component of dicot wood
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additional information
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synthesis of xylooligosaccharides from Xyl(6)-anthranilic acid by XylT activity in microsomal fractions of stems from irx9 mutant plants: after up to 5 h mixture of Xyl(7) to Xyl(9) only, Xyl(7) predominates
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additional information
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synthesis of xylooligosaccharides from Xyl(6)-anthranilic acid by XylT activity in microsomal fractions of stems from wild-type plants: after 2 h Xyl(7) to Xyl(9) predominate, after 5 h increased proportion of Xyl(10) to Xyl(12)
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additional information
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Xyl(1)-anthranilic acid is not acceptor for XylT activity in microsomal fractions of stems from wild-type plants
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additional information
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Xyl(2)-anthranilic acid is a weak acceptor for XylT activity in microsomal fractions of stems from wild-type plants
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additional information
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Xyl(3)-anthranilic acid to Xyl(6)-anthranilic acid are readily accepted by XylT activity in microsomal fractions of stems from wild-type plants
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additional information
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Xyl(4)-anthranilic acid is acceptor for synthesis of xylooligosaccharides of sizes from Xyl(5) to Xyl(11) by XylT activity in microsomal fractions of stems from wild-type plants within 2 h, prolonged incubation time (5-8 h) leads to increased proportion of Xyl(9) to Xyl(11) but not to synthesis of longer xylooligosaccharides
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additional information
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no activity with UDP-glucose, UDP-arabinopyranose, or UDP-arabinofuranose
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brenda
cell layer of middle stem between second xylem interfasicular region and cortex corresponding to vascular cambium, ATCSLD5 promoter driven beta-glucuronidase (GUS) expression
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vasicular tissue, ATCSLD5 promoter driven beta-glucuronidase (GUS) expression
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vasicular tissue, ATCSLD5 promoter driven beta-glucuronidase (GUS) expression
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high levels, otherwise ubiquitous (in silico analysis)
brenda
weak expression in cortical and pith cells, ATCSLD5 promoter driven beta-glucuronidase (GUS) expression
brenda
weak expression, ATCSLD5 promoter driven beta-glucuronidase (GUS) expression, possibly false positive due to loading of GUS reaction products via symplastic connections
brenda
shorter in absence of ATCSLD5 (ATCSLD5-1 mutant line) compared to wild-type plants, severe reduction in root length in presence of cellulose synthase inhibitor isoxaben abolished by complementation with wild-type ATCSLD5
brenda
vasicular tissue, ATCSLD5 promoter driven beta-glucuronidase (GUS) expression
brenda
high levels, otherwise ubiquitous (in silico analysis)
brenda
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brenda
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beta-1,4-D-xylan synthase is reduced by about 40% in qua1-1 stems relative to wild type
brenda
ATCSLD5-1 mutant line: reaction product 1,4-beta-D-xylan reduction most pronounced in top portion (independent of stem hight), absent from interfasicular tissue as revealed by immunocytochemistry
brenda
reaction product 1,4-beta-D-xylan detectable in top, middle and base portion, present in xylem and interfasicular tissue as revealed by immunocytochemistry, ATCSLD5 most abundant during stem elongation between day 21 and 25 (in silico analysis)
brenda
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XylT activity in microsomal fraction only in presence of exogenous Xyl(6), wild-type plant
brenda
additional information
absent from flowers and siliques, ATCSLD5 promoter driven beta-glucuronidase (GUS) expression
brenda
additional information
complete loss of ATCSLD5 expression in mutant line atcsld5-1 with T-DNA homozygote insertion in first exon of ATCSLD5 gene, revealed by RT-PCR from 6-week old plants
brenda
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Orfila, C.; S?rensen, S.O.; Harholt, J.; Geshi, N.; Crombie, H.; Truong, H.N.; Reid, J.S.; Knox, J.P.; Scheller, H.V.
QUASIMODO1 is expressed in vascular tissue of Arabidopsis thaliana inflorescence stems, and affects homogalacturonan and xylan biosynthesis
Planta
222
613-622
2005
Arabidopsis thaliana
brenda
Lee, C.; ONeill, M.A.; Tsumuraya, Y.; Darvill, A.G.; Ye, Z.
The irregular xylem9 mutant is deficient in xylan xylosyltransferase activity
Plant Cell Physiol.
48
1624-1634
2007
Arabidopsis thaliana
brenda
Bernal, A.J.; Jensen, J.K.; Harholt, J.; S?rensen, S.; Moller, I.; Blaukopf, C.; Johansen, B.; de Lotto, R.; Pauly, M.; Scheller, H.V.; Willats, W.G.
Disruption of ATCSLD5 results in reduced growth, reduced xylan and homogalacturonan synthase activity and altered xylan occurrence in Arabidopsis
Plant J.
52
791-802
2007
Arabidopsis thaliana (Q9SRW9)
brenda
Lee, C.; Zhong, R.; Ye, Z.H.
Arabidopsis family GT43 members are xylan xylosyltransferases required for the elongation of the xylan backbone
Plant Cell Physiol.
53
135-143
2012
Arabidopsis thaliana (Q8L707), Arabidopsis thaliana (Q9ZQC6), Arabidopsis thaliana
brenda
Jensen, J.K.; Johnson, N.R.; Wilkerson, C.G.
Arabidopsis thaliana IRX10 and two related proteins from psyllium and Physcomitrella patens are xylan xylosyltransferases
Plant J.
80
207-215
2014
Arabidopsis thaliana, Physcomitrium patens, Plantago ovata
brenda
Hsieh, Y.S.Y.; Harris, P.J.
Xylans of red and green algae What is known about their structures and how they are synthesized?
Polymers (Basel)
11
354
2019
Arabidopsis thaliana, Klebsormidium flaccidum
brenda