Activating Compound | Comment | Organism | Structure |
---|---|---|---|
UDP-D-glucuronic acid | 30% increased XylT activity in microsomal fractions of stems from wild-type plants possibly due to increased solubility of xylooligomers | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.6 | - |
(1,4-beta-D-xylopyranose)6 | microsomal fractions of stems from wild-type plants | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | activity in microsomal fractions enriched with Golgi marker enzyme inosin diphosphatase | Arabidopsis thaliana | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Arabidopsis thaliana | xylosyltransferase (XylT) activity, biosynthesis of xylan, a major component of dicot wood | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
ecotype Columbia: wild-type plant, mutant plant irx9 (T-DNA insertion (SALK_058238) in the irx9 mutant disrupts an IRX9 exon that encodes part of the putative catalytic domain), or mutant plant irx9 complemented with IRX9 wild-type protein | - |
Purification (Comment) | Organism |
---|---|
activity in microsomal fractions from stems, roots, leaves and seedlings, homogenized with mortar and pestle in 50 mM HEPES pH7.3, 0.4 M sucrose followed by differential centrifugation, storage at -80°C | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
stem | XylT activity in microsomal fraction only in presence of exogenous Xyl(6), wild-type plant | Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
substantial decrease in XylT activity and deficiency in elongation of xylooligosaccharides in microsomal fractions of stems from irx9 mutant plants | Arabidopsis thaliana |
0.0000218 | - |
with respect to (1,4-beta-D-xylopyranose)6, microsomal fractions of stems from wild-type plants | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | xylosyltransferase (XylT) activity, biosynthesis of xylan, a major component of dicot wood | Arabidopsis thaliana | ? | - |
? | |
additional information | synthesis of xylooligosaccharides from Xyl(6)-anthranilic acid by XylT activity in microsomal fractions of stems from irx9 mutant plants: after up to 5 h mixture of Xyl(7) to Xyl(9) only, Xyl(7) predominates | Arabidopsis thaliana | ? | - |
? | |
additional information | synthesis of xylooligosaccharides from Xyl(6)-anthranilic acid by XylT activity in microsomal fractions of stems from wild-type plants: after 2 h Xyl(7) to Xyl(9) predominate, after 5 h increased proportion of Xyl(10) to Xyl(12) | Arabidopsis thaliana | ? | - |
? | |
additional information | Xyl(1)-anthranilic acid is not acceptor for XylT activity in microsomal fractions of stems from wild-type plants | Arabidopsis thaliana | ? | - |
? | |
additional information | Xyl(2)-anthranilic acid is a weak acceptor for XylT activity in microsomal fractions of stems from wild-type plants | Arabidopsis thaliana | ? | - |
? | |
additional information | Xyl(3)-anthranilic acid to Xyl(6)-anthranilic acid are readily accepted by XylT activity in microsomal fractions of stems from wild-type plants | Arabidopsis thaliana | ? | - |
? | |
additional information | Xyl(4)-anthranilic acid is acceptor for synthesis of xylooligosaccharides of sizes from Xyl(5) to Xyl(11) by XylT activity in microsomal fractions of stems from wild-type plants within 2 h, prolonged incubation time (5-8 h) leads to increased proportion of Xyl(9) to Xyl(11) but not to synthesis of longer xylooligosaccharides | Arabidopsis thaliana | ? | - |
? | |
UDP-D-xylose + ((1,4-beta-D-xylooligosaccharide)n)-anthranilic acid | 0.1 mM UDP-xylose + 0.5 mM anthranilic acid (AA) labelled xylooligosaccharides (Xyl(2) to Xyl(6)) as acceptor, 200 microgram microsomes, 21°C, pH 6.8, in presence of 5 mM MnCl2, 1 mM dithiothreitol, 0.5% Triton X-100 | Arabidopsis thaliana | UDP + ((1,4-beta-D-xylooligosaccharide)n+1)-anthranilic acid | xylooligosaccharides (up to Xyl(12)) purified by HPLC and analysed by MALDI-TOF mass spectrometry | ? | |
UDP-D-xylose + (1,4-beta-D-xylooligosaccharide)n | 0.1 mM radiolabelled UDP-D-xylose + 0.2 microgram/microlitre (1,4-beta-D-xylopyranose)6, 100 microgram microsomes, 21°C, pH 6.8, in presence of 5 mM MnCl2, 1 mM dithiothreitol, 0.5% Triton X-100, reaction dependent on time and protein concentration | Arabidopsis thaliana | UDP + (1,4-beta-D-xylooligosaccharide)n+1 | reduction in XylT activity in microsomal fraction of stems from irx9 mutant plants compared to wild-type plants in presence of exogenous substrates can be abolished by complementation with wild-type IRX9 gene, product quantification by scintillation counting | ? | |
UDP-D-xylose + (1,4-beta-D-xylopyranose)6 | - |
Arabidopsis thaliana | UDP + (1,4-beta-D-xylopyranose)7 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
irregular xylem9 | - |
Arabidopsis thaliana |
IRX9 | - |
Arabidopsis thaliana |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 20 | microsomal fractions of stems from wild-type plants | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
microsomal fractions of stems from wild-type plants | Arabidopsis thaliana |