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Search Purification (Commentary)

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EC Number Purification (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 2.4.2.24activity in microsomal fractions from stems, roots, leaves and seedlings, homogenized with mortar and pestle in 50 mM HEPES pH7.3, 0.4 M sucrose followed by differential centrifugation, storage at -80°C
Display the word mapDisplay the reaction diagram Show all sequences 2.4.2.24GFP-Trap A agarose resin column chromatography
Display the word mapDisplay the reaction diagram Show all sequences 2.4.2.24HisTap column chromatography
Display the word mapDisplay the reaction diagram Show all sequences 2.4.2.24microsomal extraction by differential centrifugation using 50 mM HEPES-NaOH pH7.3, 400 microM sucrose, 4 mM dithiothreitol, EDTA for beta-(1,4)-xylan synthase activity measurements
Display the word mapDisplay the reaction diagram Show all sequences 2.4.2.24preparation of microsomal fraction
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