development of enzyme-bound solid-phase support containing long-pectic oligogalacturonides anchored to the resin material via disulfide-containing cleavable linker, enzyme is reversibly bound, for performance of solid-phase biosynthetic reaction to investigate the pectin oligomer biosynthesis, product identification by MALDI-TOF mass spectrometry, overview
Doong, R.L.; Liljebjelke, K.; Fralish, G.; Kumar, A.; Mohnen, D.
Cell-free synthesis of pectin. Identification and partial characterization of polygalaturonate 4-alpha-galaturonosyltransferase and its products from membrane preparations of tobacco cell-suspension cultures
Akita, K.; Ishimizu, T.; Tsukamoto, T.; Ando, T.; Hase, S.
Successive glycosyltransfer activity and enzymatic characterization of pectic polygalacturonate 4-alpha-galacturonosyltransferase solubilized from pollen tubes of Petunia axillaris using pyridylaminated oligogalacturonates as substrates