Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.43 extracted from

  • Villemez, C.L.; Swanson, A.L.; Hassid, W.Z.
    Properties of a polygalacturonic acid-synthesizing enzyme system from Phaseolus aureus seedlings (1966), Arch. Biochem. Biophys., 116, 446-452.
    View publication on PubMed
Search for more literature for 2.4.1.43

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin necessary in homogenization media Vigna radiata var. radiata
sucrose 400 mM, 5fold increase of activity Vigna radiata var. radiata

General Stability

General Stability Organism
presence of bovine serum albumin is essential for preparation Vigna radiata var. radiata

Inhibitors

Inhibitors Comment Organism Structure
CaCl2 weak Vigna radiata var. radiata
CuSO4 strong Vigna radiata var. radiata
diphosphate weak Vigna radiata var. radiata
HgCl2 strong Vigna radiata var. radiata
UDP strong Vigna radiata var. radiata
UMP strong Vigna radiata var. radiata
UTP strong Vigna radiata var. radiata
ZnCl2 weak Vigna radiata var. radiata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0017
-
 UDP-D-galacturonic acid
-
 Vigna radiata var. radiata

Metals/Ions

Metals/Ions Comment Organism Structure
CoCl2 slight stimulation Vigna radiata var. radiata
KCl slight stimulation Vigna radiata var. radiata
MgCl2 slight stimulation Vigna radiata var. radiata
MnCl2 stimulation, optimal concentration: 1.7 mM Vigna radiata var. radiata
MnSO4 stimulation Vigna radiata var. radiata
Na2SO4 slight stimulation Vigna radiata var. radiata
NaCl slight stimulation Vigna radiata var. radiata
NH4Cl slight stimulation Vigna radiata var. radiata

Organism

Organism UniProt Comment Textmining
Vigna radiata var. radiata
-
 mung beans
-

Purification (Commentary)

Purification (Comment) Organism
partial Vigna radiata var. radiata

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Vigna radiata var. radiata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 highest specific activity in seedlings germinated for 3 days Vigna radiata var. radiata

Storage Stability

Storage Stability Organism
-18°C, 1,7 mM MnCl2, 11 days, 35% loss of activity Vigna radiata var. radiata
-18°C, approx. 3 weeks, complete inactivation Vigna radiata var. radiata
-18°C, without MnCl2, 11 days, 60% loss of activity Vigna radiata var. radiata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-D-galacturonic acid + (1,4-alpha-D-galacturonosyl)n
-
 Vigna radiata var. radiata UDP + (1,4-alpha-D-galacturonosyl)n+1
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Vigna radiata var. radiata

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
-
 24 h, complete inactivation Vigna radiata var. radiata
30
-
 20 min, complete inactivation Vigna radiata var. radiata
37
-
 5 min, complete inactivation Vigna radiata var. radiata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Vigna radiata var. radiata

pH Range

pH Minimum pH Maximum Comment Organism
5.3 7 33% of maximal activity at pH 5.3, 35% of maximal activity at pH 7.0 Vigna radiata var. radiata