Literature summary for 2.4.1.43 extracted from

Doong, R.L.; Liljebjelke, K.; Fralish, G.; Kumar, A.; Mohnen, D.
Cell-free synthesis of pectin. Identification and partial characterization of polygalaturonate 4-alpha-galaturonosyltransferase and its products from membrane preparations of tobacco cell-suspension cultures (1995), Plant Physiol., 109, 141-152.

Search for more literature for 2.4.1.43

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0089	-	UDP-D-galacturonic acid	-	Nicotiana tabacum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Metals/Ions

Metals/Ions	Comment	Organism	Structure
KCl	25 mM, 76% stimulation	Nicotiana tabacum
MnCl2	maximal activation at 0.25 mM	Nicotiana tabacum
NaCl	25 mM, 76% stimulation	Nicotiana tabacum

Organism

Organism	UniProt	Comment	Textmining
Nicotiana tabacum	-	L. cv. Samsun	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
cell suspension culture	established from pith callus tissue	Nicotiana tabacum	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-D-galacturonic acid + (1,4-alpha-D-galacturonosyl)n	-	Nicotiana tabacum	UDP + (1,4-alpha-D-galacturonosyl)n+1	at least 34-89% alpha-1,4-linked homogalacturonan of approx. 105000 Da	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	30	second minor optimum at 45 or 50°C	Nicotiana tabacum

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
50	-	30 min, complete inactivation	Nicotiana tabacum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.8	-	-	Nicotiana tabacum

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)