Information on EC 2.4.1.33 - mannuronan synthase

for references in articles please use BRENDA:EC2.4.1.33
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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

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EC NUMBER
COMMENTARY hide
2.4.1.33
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RECOMMENDED NAME
GeneOntology No.
mannuronan synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
GDP-alpha-D-mannuronate + [(1->4)-beta-D-mannuronosyl]n = GDP + [(1->4)-beta-D-mannuronosyl]n+1
show the reaction diagram
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-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
alginate biosynthesis I (algal)
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alginate biosynthesis II (bacterial)
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alginate biosynthesis
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Fructose and mannose metabolism
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SYSTEMATIC NAME
IUBMB Comments
GDP-alpha-D-mannuronate:mannuronan D-mannuronatetransferase
The enzyme catalyses the polymerization of beta-D-mannuronate residues into a mannuronan polymer, an intermediate in the biosynthesis of alginate. It is found in brown algae and in alginate-producing bacterial species from the Pseudomonas and Azotobacter genera.
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CAS REGISTRY NUMBER
COMMENTARY hide
37257-31-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
marine brown alga
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-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
alpha-D-glucuronate 1-phosphate + (alginate)n
phosphate + ?
show the reaction diagram
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25% of activity with GDP-D-mannuronate
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-
?
alpha-D-mannuronate 1-phosphate + (alginate)n
phosphate + (alginate)n+1
show the reaction diagram
-
25% of activity with GDP-D-mannuronate
-
-
?
GDP-D-mannuronate + (alginate)n
GDP + (alginate)n+1
show the reaction diagram
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
GDP-D-mannuronate + (alginate)n
GDP + (alginate)n+1
show the reaction diagram
-
-
-
-
ir
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MnCl2
-
no essential requirement, at 0.25 mM: 15% increase of activity, inhibition above 0.75 mM
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ATP
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5% inhibition
MnCl2
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at 0.25 mM: 15% increase of activity, above 0.75 mM: 80% inhibition
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5'-GMP
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slight stimulation
GDP
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0.3 mM: 15% stimulation
additional information
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GTP, UTP, CTP, TTP, a mixture of NAD+ and NADP+ have no significant effect
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7
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-
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.6 - 8.5
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65% of maximal activity at pH 5.6 and pH 8.5
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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PDB
SCOP
CATH
ORGANISM
UNIPROT
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10
-
15 min, 23% remaining activity
36
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15 min, 72% remaining activity
45
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15 min, 60% remaining activity
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, 3 days, 80% remaining activity
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0-4°C, unstable
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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LINKS TO OTHER DATABASES (specific for EC-Number 2.4.1.33)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)