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Literature summary for 2.4.1.33 extracted from

  • Lin, T.Y.; Hassid, W.Z.
    Pathway of alginic acid synthesis in the marine brown alga, Fucus gardneri Silva (1966), J. Biol. Chem., 241, 5284-5297.
    View publication on PubMed
Search for more literature for 2.4.1.33

Activating Compound

Activating Compound Comment Organism Structure
5'-GMP slight stimulation Fucus gardneri
GDP 0.3 mM: 15% stimulation Fucus gardneri
additional information GTP, UTP, CTP, TTP, a mixture of NAD+ and NADP+ have no significant effect Fucus gardneri

Inhibitors

Inhibitors Comment Organism Structure
ATP 5% inhibition Fucus gardneri
MnCl2 at 0.25 mM: 15% increase of activity, above 0.75 mM: 80% inhibition Fucus gardneri

Metals/Ions

Metals/Ions Comment Organism Structure
MnCl2 no essential requirement, at 0.25 mM: 15% increase of activity, inhibition above 0.75 mM Fucus gardneri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP-D-mannuronate + (alginate)n Fucus gardneri
-
 GDP + (alginate)n+1
-
 ir

Organism

Organism UniProt Comment Textmining
Fucus gardneri
-
 marine brown alga
-

Purification (Commentary)

Purification (Comment) Organism
partial Fucus gardneri

Source Tissue

Source Tissue Comment Organism Textmining
thallus
-
 Fucus gardneri
-

Storage Stability

Storage Stability Organism
-15°C, 3 days, 80% remaining activity Fucus gardneri
0-4°C, unstable Fucus gardneri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-glucuronate 1-phosphate + (alginate)n 25% of activity with GDP-D-mannuronate Fucus gardneri phosphate + ?
-
 ?
alpha-D-mannuronate 1-phosphate + (alginate)n 25% of activity with GDP-D-mannuronate Fucus gardneri phosphate + (alginate)n+1
-
 ?
GDP-D-mannuronate + (alginate)n
-
 Fucus gardneri GDP + (alginate)n+1
-
 ir

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
36
-
-
 Fucus gardneri

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10
-
 15 min, 23% remaining activity Fucus gardneri
36
-
 15 min, 72% remaining activity Fucus gardneri
45
-
 15 min, 60% remaining activity Fucus gardneri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
 Fucus gardneri

pH Range

pH Minimum pH Maximum Comment Organism
5.6 8.5 65% of maximal activity at pH 5.6 and pH 8.5 Fucus gardneri