We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Information on EC 2.4.1.271 - crocetin glucosyltransferase for references in articles please use BRENDA:EC2.4.1.271Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
The enzyme appears in viruses and cellular organisms
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
crocetin glucosyltransferase
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-alpha-D-glucose + beta-D-gentiobiosyl crocetin = UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
(3)
-
-
-
UDP-alpha-D-glucose + beta-D-glucosyl crocetin = UDP + bis(beta-D-glucosyl) crocetin
(2)
-
-
-
UDP-alpha-D-glucose + crocetin = UDP + beta-D-glucosyl crocetin
(1)
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
crocetin esters biosynthesis
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-glucose:crocetin 8-O-D-glucosyltransferase
In the plants Crocus sativus and Gardenia jasminoides this enzyme esterifies a free carboxyl group of crocetin and some crocetin glycosyl esters. The enzyme from Gardenia can also form glucosyl esters with 4-coumarate, caffeate and ferulate [3].
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
crocetin glucosyltransferase
crocetin glucosyltransferase 2
UDP-glucose-crocetin 8,8'-glucosyltransferase
-
-
UDPGlc:crocetin 8,8'-glucosyltransferase
-
-
uridine-5'-diphosphoglucose-crocetin 8,8'-glucosyltransferase
-
-
crocetin glucosyltransferase
-
-
crocetin glucosyltransferase
-
-
crocetin glucosyltransferase
-
-
crocetin glucosyltransferase
-
-
crocetin glucosyltransferase
-
-
crocetin glucosyltransferase
-
-
crocetin glucosyltransferase
-
-
crocetin glucosyltransferase 2
-
-
crocetin glucosyltransferase 2
-
crocetin glucosyltransferase 2
-
crocetin GTase
-
-
-
-
GLT2
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
UniProt
brenda
-
-
-
brenda
-
UniProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
physiological function
-
the enzyme is involved in the biosynthesis of crocetin monoglucosyl- and diglucosyl-esters. Glycosylation of crocetin into crocin involves more than one glucosyltransferase. GTASE1 catalyzes the glucose transfer on both carboxylic ends of crocetin and GTASE2 catalyzes the formation of glucosidic bonds with formation of gentiobiosyl esters
physiological function
-
the enzyme participates in the biosynthesis of crocin
physiological function
-
two glucosyltransferases might be involved in the formation of crocetin glucosyl- and gentiobiosyl-esters. GTase1 formed an ester bond between crocetin carboxyl groups and glucose moieties while GTase2 catalyzed the formation of glucosidic bonds with glucosyl ester groups at both ends of the molecule
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-alpha-D-glucose + beta-D-gentiobiosyl crocetin
UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
UDP-glucose + beta-D-gentiobiosyl crocetin
UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
-
-
-
-
?
UDP-glucose + beta-D-glucosyl crocetin
UDP + bis(beta-D-glucosyl) crocetin
-
-
-
-
?
UDP-glucose + crocetin
UDP + beta-D-glucosyl crocetin
additional information
?
-
-
the enzyme does not add other glucose units to the glucosyl-esters to form crocetin gentiobiosyl-esters
-
-
-
UDP-alpha-D-glucose + beta-D-gentiobiosyl crocetin
UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
-
-
-
-
?
UDP-alpha-D-glucose + beta-D-gentiobiosyl crocetin
UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
-
-
-
?
UDP-alpha-D-glucose + beta-D-gentiobiosyl crocetin
UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
-
-
-
?
UDP-glucose + crocetin
UDP + beta-D-glucosyl crocetin
-
-
-
-
?
UDP-glucose + crocetin
UDP + beta-D-glucosyl crocetin
-
the enzyme participates in the biosynthesis of crocin
-
-
?
UDP-glucose + crocetin
UDP + beta-D-glucosyl crocetin
-
the enzyme is specific for crocetin
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-alpha-D-glucose + beta-D-gentiobiosyl crocetin
UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
UDP-glucose + beta-D-gentiobiosyl crocetin
UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
-
-
-
-
?
UDP-glucose + beta-D-glucosyl crocetin
UDP + bis(beta-D-glucosyl) crocetin
-
-
-
-
?
UDP-glucose + crocetin
UDP + beta-D-glucosyl crocetin
UDP-alpha-D-glucose + beta-D-gentiobiosyl crocetin
UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
-
-
-
-
?
UDP-alpha-D-glucose + beta-D-gentiobiosyl crocetin
UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
Q6X1C0
-
-
-
?
UDP-alpha-D-glucose + beta-D-gentiobiosyl crocetin
UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
Q6X1C0
-
-
-
?
UDP-glucose + crocetin
UDP + beta-D-glucosyl crocetin
-
-
-
-
?
UDP-glucose + crocetin
UDP + beta-D-glucosyl crocetin
-
the enzyme participates in the biosynthesis of crocin
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2-mercaptoethanol
-
10 mM, 69% inhibition
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.17
crocetin
-
pH 7.1, 40°C
0.72
UDP-glucose
-
pH 7.1, 40°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
40
-
asay at
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
35 - 45
-
35°C: about 75% of maximal activity, 45°C: about 85% of maximal activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
4.4 - 4.6
-
the 300fold purified extract is subjected to isoelectric focusing and gives three active GTASE1 bands at pH 4.4, 4.5, and 4.6
additional information
-
the most purified preparation shows isoforms between pH 4.4 and 4.8 when resolved by isoelectric focusing
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
brenda
-
brenda
-
brenda
-
-
brenda
-
brenda
-
-
brenda
-
-
brenda
-
-
brenda
-
-
brenda
-
UGTCs2 is mainly expressed in fully developed stigmas
brenda
-
-
brenda
-
-
brenda
-
-
brenda
-
-
brenda
-
-
brenda
-
-
brenda
-
-
brenda
-
-
brenda
-
-
brenda
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
26000
-
2 * 26000, SDS-PAGE
49000 - 55000
-
gel filtrration
50700
-
x * 50700, calculated from sequence
49400
-
x * 49400, SDS-PAGE
49400
x * 49400, SDS-PAGE
49400
x * 49400, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
dimer
-
2 * 26000, SDS-PAGE
?
-
x * 49400, SDS-PAGE
?
-
x * 50700, calculated from sequence
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
purified 125fold trough a multi-step process of acetone fractionation, anion-exchange, and gel filtration chromatography
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expression as His-tag fusion protein in Escherichia coli
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GTase1 activity is higher during the first four days of crocin glucosides biosynthesis, but decreases after four days
-
treatment with jasmonic acid or salicylic acid, drough, wounding and dehydration fail to affect the expression levels of UGTCs2
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GLT2_CROSA
460
50722
Swiss-Prot
GLT3_CROSA
475
52650
Swiss-Prot
UGT1_GARJA
474
53008
Swiss-Prot
A0A2P6QVX9_ROSCH
162
18315
TrEMBL
A0A2U8XEJ8_CROSA
459
51048
TrEMBL
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Cote, F.; Cormier, F.; Dufresne, C.; Willemot, C.
A highly specific glucosyltransferase is involved in the synthesis of crocetin glucosylesters in Crocus sativus cultured cells
J. Plant Physiol.
158
553-560
2001
Crocus sativus
-
brenda
Cote, F.; Cormier, F.; Dufresne, C.; Willemot, C.
Properties of a glucosyltransferase involved in crocin synthesis
Plant Sci.
153
55-63
2000
Crocus sativus
brenda
Moraga, A.R.; Nohales, P.F.; Perez, J.A.; Gomez-Gomez, L.
Glucosylation of the saffron apocarotenoid crocetin by a glucosyltransferase isolated from Crocus sativus stigmas
Planta
219
955-966
2004
Crocus sativus
brenda
Yang, B.; Guo, Z.; Liu, R.
Crocin synthesis mechanism in Crocus sativus
Tsinghua Sci. Technol.
10
567-572
2005
Crocus sativus
-
brenda
Paredi, G.; Raboni, S.; Marchesani, F.; Ordoudi, S.A.; Tsimidou, M.Z.; Mozzarelli, A.
Insight of saffron proteome by gel-electrophoresis
Molecules
21
167
2016
Carthamus tinctorius, Crocus sativus, Crocus sativus (Q6X1C0), Gardenia jasminoides, Gardenia jasminoides (Q6X1C0)
brenda
Rubio Moraga, A.; Ahrazem, O.; Rambla, J.L.; Granell, A.; Gomez Gomez, L.
Crocins with high levels of sugar conjugation contribute to the yellow colours of early-spring flowering crocus tepals
PLoS ONE
8
e71946
2013
Crocus angustifolius, Crocus chrysanthus, Crocus korolkowii, Crocus olivieri, Crocus sativus, Crocus sieberi, Crocus vitellinus
brenda
html completed