Information on EC 2.4.1.271 - crocetin glucosyltransferase

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The enzyme appears in viruses and cellular organisms

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EC NUMBER
COMMENTARY hide
2.4.1.271
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RECOMMENDED NAME
GeneOntology No.
crocetin glucosyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-alpha-D-glucose + beta-D-gentiobiosyl crocetin = UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
show the reaction diagram
(3)
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-
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UDP-alpha-D-glucose + beta-D-glucosyl crocetin = UDP + bis(beta-D-glucosyl) crocetin
show the reaction diagram
(2)
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-
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UDP-alpha-D-glucose + crocetin = UDP + beta-D-glucosyl crocetin
show the reaction diagram
(1)
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
crocetin esters biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:crocetin 8-O-D-glucosyltransferase
In the plants Crocus sativus and Gardenia jasminoides this enzyme esterifies a free carboxyl group of crocetin and some crocetin glycosyl esters. The enzyme from Gardenia can also form glucosyl esters with 4-coumarate, caffeate and ferulate [3].
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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-
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + beta-D-gentiobiosyl crocetin
UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
show the reaction diagram
UDP-glucose + beta-D-gentiobiosyl crocetin
UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
show the reaction diagram
-
-
-
-
?
UDP-glucose + beta-D-glucosyl crocetin
UDP + bis(beta-D-glucosyl) crocetin
show the reaction diagram
-
-
-
-
?
UDP-glucose + crocetin
UDP + beta-D-glucosyl crocetin
show the reaction diagram
additional information
?
-
-
the enzyme does not add other glucose units to the glucosyl-esters to form crocetin gentiobiosyl-esters
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-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + beta-D-gentiobiosyl crocetin
UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
show the reaction diagram
UDP-glucose + beta-D-gentiobiosyl crocetin
UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
show the reaction diagram
-
-
-
-
?
UDP-glucose + beta-D-glucosyl crocetin
UDP + bis(beta-D-glucosyl) crocetin
show the reaction diagram
-
-
-
-
?
UDP-glucose + crocetin
UDP + beta-D-glucosyl crocetin
show the reaction diagram
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
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10 mM, 69% inhibition
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.17
crocetin
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pH 7.1, 40°C
0.72
UDP-glucose
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pH 7.1, 40°C
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.6
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pH 7.1, 40°C
10.8
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pH 7.1, 40°C
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
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assay at
7.1
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assay at
7.4
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assay at
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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assay at
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35 - 45
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35°C: about 75% of maximal activity, 45°C: about 85% of maximal activity
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.4 - 4.6
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the 300fold purified extract is subjected to isoelectric focusing and gives three active GTASE1 bands at pH 4.4, 4.5, and 4.6
additional information
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the most purified preparation shows isoforms between pH 4.4 and 4.8 when resolved by isoelectric focusing
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
26000
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2 * 26000, SDS-PAGE
47000
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gel filtration
49000 - 55000
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gel filtrration
50700
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x * 50700, calculated from sequence
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
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2 * 26000, SDS-PAGE
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purified 125fold trough a multi-step process of acetone fractionation, anion-exchange, and gel filtration chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression as His-tag fusion protein in Escherichia coli
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
GTase1 activity is higher during the first four days of crocin glucosides biosynthesis, but decreases after four days
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treatment with jasmonic acid or salicylic acid, drough, wounding and dehydration fail to affect the expression levels of UGTCs2
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LINKS TO OTHER DATABASES (specific for EC-Number 2.4.1.271)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)