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Literature summary for 2.4.1.271 extracted from

  • Cote, F.; Cormier, F.; Dufresne, C.; Willemot, C.
    Properties of a glucosyltransferase involved in crocin synthesis (2000), Plant Sci., 153, 55-63.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 10 mM, 69% inhibition Crocus sativus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
crocetin pH 7.1, 40°C Crocus sativus
0.72
-
UDP-glucose pH 7.1, 40°C Crocus sativus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
2 * 26000, SDS-PAGE Crocus sativus
47000
-
gel filtration Crocus sativus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + crocetin Crocus sativus the enzyme participates in the biosynthesis of crocin UDP + beta-D-glucosyl crocetin
-
?

Organism

Organism UniProt Comment Textmining
Crocus sativus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purified 125fold trough a multi-step process of acetone fractionation, anion-exchange, and gel filtration chromatography Crocus sativus

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
Crocus sativus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6
-
pH 7.1, 40°C Crocus sativus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + crocetin
-
Crocus sativus UDP + beta-D-glucosyl crocetin
-
?
UDP-glucose + crocetin the enzyme participates in the biosynthesis of crocin Crocus sativus UDP + beta-D-glucosyl crocetin
-
?

Subunits

Subunits Comment Organism
dimer 2 * 26000, SDS-PAGE Crocus sativus

Synonyms

Synonyms Comment Organism
crocetin GTase
-
Crocus sativus
UDP-glucose-crocetin 8,8'-glucosyltransferase
-
Crocus sativus
uridine-5'-diphosphoglucose-crocetin 8,8'-glucosyltransferase
-
Crocus sativus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Crocus sativus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 45 35°C: about 75% of maximal activity, 45°C: about 85% of maximal activity Crocus sativus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1
-
assay at Crocus sativus

pI Value

Organism Comment pI Value Maximum pI Value
Crocus sativus the most purified preparation shows isoforms between pH 4.4 and 4.8 when resolved by isoelectric focusing
-
additional information

General Information

General Information Comment Organism
physiological function the enzyme participates in the biosynthesis of crocin Crocus sativus