Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 10 mM, 69% inhibition | Crocus sativus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
crocetin | pH 7.1, 40°C | Crocus sativus | |
0.72 | - |
UDP-glucose | pH 7.1, 40°C | Crocus sativus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
2 * 26000, SDS-PAGE | Crocus sativus |
47000 | - |
gel filtration | Crocus sativus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + crocetin | Crocus sativus | the enzyme participates in the biosynthesis of crocin | UDP + beta-D-glucosyl crocetin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Crocus sativus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purified 125fold trough a multi-step process of acetone fractionation, anion-exchange, and gel filtration chromatography | Crocus sativus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Crocus sativus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.6 | - |
pH 7.1, 40°C | Crocus sativus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + crocetin | - |
Crocus sativus | UDP + beta-D-glucosyl crocetin | - |
? | |
UDP-glucose + crocetin | the enzyme participates in the biosynthesis of crocin | Crocus sativus | UDP + beta-D-glucosyl crocetin | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 26000, SDS-PAGE | Crocus sativus |
Synonyms | Comment | Organism |
---|---|---|
crocetin GTase | - |
Crocus sativus |
UDP-glucose-crocetin 8,8'-glucosyltransferase | - |
Crocus sativus |
uridine-5'-diphosphoglucose-crocetin 8,8'-glucosyltransferase | - |
Crocus sativus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Crocus sativus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 45 | 35°C: about 75% of maximal activity, 45°C: about 85% of maximal activity | Crocus sativus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
assay at | Crocus sativus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Crocus sativus | the most purified preparation shows isoforms between pH 4.4 and 4.8 when resolved by isoelectric focusing | - |
additional information |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme participates in the biosynthesis of crocin | Crocus sativus |