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Literature summary for 2.4.1.271 extracted from

  • Cote, F.; Cormier, F.; Dufresne, C.; Willemot, C.
    A highly specific glucosyltransferase is involved in the synthesis of crocetin glucosylesters in Crocus sativus cultured cells (2001), J. Plant Physiol., 158, 553-560.
No PubMed abstract available

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
2 * 26000, SDS-PAGE Crocus sativus
49000 55000 gel filtrration Crocus sativus

Organism

Organism UniProt Comment Textmining
Crocus sativus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Crocus sativus

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
Crocus sativus
-
stigma
-
Crocus sativus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.8
-
pH 7.1, 40°C Crocus sativus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme does not add other glucose units to the glucosyl-esters to form crocetin gentiobiosyl-esters Crocus sativus ?
-
?
UDP-glucose + beta-D-gentiobiosyl crocetin
-
Crocus sativus UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
-
?
UDP-glucose + beta-D-glucosyl crocetin
-
Crocus sativus UDP + bis(beta-D-glucosyl) crocetin
-
?
UDP-glucose + crocetin the enzyme is specific for crocetin Crocus sativus UDP + beta-D-glucosyl crocetin
-
?

Subunits

Subunits Comment Organism
dimer 2 * 26000, SDS-PAGE Crocus sativus

Synonyms

Synonyms Comment Organism
GTASE1
-
Crocus sativus
UDPGlc:crocetin 8,8'-glucosyltransferase
-
Crocus sativus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
asay at Crocus sativus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1
-
assay at Crocus sativus

pI Value

Organism Comment pI Value Maximum pI Value
Crocus sativus the 300fold purified extract is subjected to isoelectric focusing and gives three active GTASE1 bands at pH 4.4, 4.5, and 4.6 4.6 4.4

General Information

General Information Comment Organism
physiological function the enzyme is involved in the biosynthesis of crocetin monoglucosyl- and diglucosyl-esters. Glycosylation of crocetin into crocin involves more than one glucosyltransferase. GTASE1 catalyzes the glucose transfer on both carboxylic ends of crocetin and GTASE2 catalyzes the formation of glucosidic bonds with formation of gentiobiosyl esters Crocus sativus