Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
2 * 26000, SDS-PAGE | Crocus sativus |
49000 | 55000 | gel filtrration | Crocus sativus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Crocus sativus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Crocus sativus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Crocus sativus | - |
stigma | - |
Crocus sativus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.8 | - |
pH 7.1, 40°C | Crocus sativus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme does not add other glucose units to the glucosyl-esters to form crocetin gentiobiosyl-esters | Crocus sativus | ? | - |
? | |
UDP-glucose + beta-D-gentiobiosyl crocetin | - |
Crocus sativus | UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin | - |
? | |
UDP-glucose + beta-D-glucosyl crocetin | - |
Crocus sativus | UDP + bis(beta-D-glucosyl) crocetin | - |
? | |
UDP-glucose + crocetin | the enzyme is specific for crocetin | Crocus sativus | UDP + beta-D-glucosyl crocetin | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 26000, SDS-PAGE | Crocus sativus |
Synonyms | Comment | Organism |
---|---|---|
GTASE1 | - |
Crocus sativus |
UDPGlc:crocetin 8,8'-glucosyltransferase | - |
Crocus sativus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
asay at | Crocus sativus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
assay at | Crocus sativus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Crocus sativus | the 300fold purified extract is subjected to isoelectric focusing and gives three active GTASE1 bands at pH 4.4, 4.5, and 4.6 | 4.6 | 4.4 |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in the biosynthesis of crocetin monoglucosyl- and diglucosyl-esters. Glycosylation of crocetin into crocin involves more than one glucosyltransferase. GTASE1 catalyzes the glucose transfer on both carboxylic ends of crocetin and GTASE2 catalyzes the formation of glucosidic bonds with formation of gentiobiosyl esters | Crocus sativus |