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Information on EC 2.4.1.223 - glucuronosyl-galactosyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase and Organism(s) Drosophila melanogaster and UniProt Accession Q9XZ08

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EC Tree
IUBMB Comments
Enzyme involved in the initiation of heparin and heparan sulfate synthesis, transferring GlcNAc to the (GlcA-Gal-Gal-Xyl-)Ser core. Apparently products of both the human EXTL2 and EXTL3 genes can catalyse this reaction. In Caenorhabditis elegans, the product of the rib-2 gene displays this activity as well as that of EC 2.4.1.224, glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase. For explanation of the use of a superscript in the systematic name, see {iupac/2carb/37#372::2-Carb-37.2}.
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This record set is specific for:
Drosophila melanogaster
UNIPROT: Q9XZ08
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Word Map
The taxonomic range for the selected organisms is: Drosophila melanogaster
The enzyme appears in selected viruses and cellular organisms
Synonyms
extl2, alpha1,4-n-acetylglucosaminyltransferase, brother of tout-velu, exostosin-like 2, galntl-1, alpha-n-acetylglucosaminyltransferase i, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
alpha-N-acetylglucosaminyltransferase I
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alpha1,4-N-acetylglucosaminyltransferase
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brother of tout-velu
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glucuronosylgalactosylproteoglycan 4-alpha-N-acetylglucosaminyltransferase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transferase
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SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-alpha-D-glucosamine:[protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine 4IV-alpha-N-acetyl-D-glucosaminyltransferase (configuration-retaining)
Enzyme involved in the initiation of heparin and heparan sulfate synthesis, transferring GlcNAc to the (GlcA-Gal-Gal-Xyl-)Ser core. Apparently products of both the human EXTL2 and EXTL3 genes can catalyse this reaction. In Caenorhabditis elegans, the product of the rib-2 gene displays this activity as well as that of EC 2.4.1.224, glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase. For explanation of the use of a superscript in the systematic name, see {iupac/2carb/37#372::2-Carb-37.2}.
CAS REGISTRY NUMBER
COMMENTARY hide
145539-84-0
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336193-98-7
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-(1-3)-beta-D-galactosyl-(1-4)-beta-D-xylosyl-proteoglycan
UDP + alpha-N-acetyl-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-(1-3)-beta-D-galactosyl-(1-4)-beta-D-xylosyl-proteoglycan
show the reaction diagram
UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-1-O-benzyloxycarbonyl
UDP + alpha-N-acetyl-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-1-O-benzyloxycarbonyl
show the reaction diagram
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?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-(1-3)-beta-D-galactosyl-(1-4)-beta-D-xylosyl-proteoglycan
UDP + alpha-N-acetyl-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-(1-3)-beta-D-galactosyl-(1-4)-beta-D-xylosyl-proteoglycan
show the reaction diagram
essential in the heparan sulfate biosynthesis
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?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene DEXT3
SwissProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
EXT3_DROME
972
1
111000
Swiss-Prot
other Location (Reliability: 3)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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enzyme null mutant, defects in Hedgehog, Wingless and Decapentaplegic signalling
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Cos-1 cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kim, B.T.; Kitagawa, H.; Tamura, J.I.; Kusche-Gullberg, M.; Lindahl, U.; Sugahara, K.
Demonstration of a novel gene DEXT3 of Drosophila melanogaster as the essential N-acetylglucosamine transferase in the heparan sulfate biosynthesis: Chain initiation and elongation
J. Biol. Chem.
277
13659-13665
2002
Drosophila melanogaster (Q9XZ08)
Manually annotated by BRENDA team
Han, C.; Belenkaya, T.Y.; Khodoun, M.; Tauchi, M.; Lin, X.; Lin, X.
Distinct and collaborative roles of Drosophila EXT family proteins in morphopgen signalling and gradient formation
Development
131
1563-1575
2005
Drosophila melanogaster
Manually annotated by BRENDA team