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Information on EC 2.4.1.131 - GDP-Man:Man3GlcNAc2-PP-dolichol alpha-1,2-mannosyltransferase and Organism(s) Schizosaccharomyces pombe and UniProt Accession O74878

for references in articles please use BRENDA:EC2.4.1.131
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IUBMB Comments
The biosynthesis of asparagine-linked glycoproteins (N-linked protein glycosylation) utilizes a dolichyl diphosphate-linked glycosyl donor, which is assembled by the series of membrane-bound glycosyltransferases that comprise the dolichol pathway. ALG11 mannosyltransferase from Saccharomyces cerevisiae carries out two sequential steps in the formation of the lipid-linked core oligosaccharide, adding two mannose residues in alpha(1->2) linkages to the nascent oligosaccharide.
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Schizosaccharomyces pombe
UNIPROT: O74878
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Word Map
  • 2.4.1.131
  • lipid-linked
  • n-glycosylation
  • n-linked
  • glycosyltransferase
  • dolichol-linked
  • asparagine-linked
  • man5glcnac2-pp-dolichol
  • alpha1,2-linked
  • single-subunit
  • oligosaccharyltransferase
  • man5glcnac2-pp-dol
  • microcephaly
The taxonomic range for the selected organisms is: Schizosaccharomyces pombe
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
alg11, halg11, gdp-man:man3glcnac2-pp-dolichol-alpha1,2-mannosyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
galactomannan deficiency protein 3
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GDP-mannose-oligosaccharide-lipid mannosyltransferase
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guanosine diphosphomannose-oligosaccharide-lipid mannosyltransferase
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mannosyltransferase, guanosine diphosphomannose-oligosaccharide-lipid
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oligosaccharide-lipid mannosyltransferase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
GDP-alpha-D-mannose:D-Man-alpha-(1->3)-[D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol 2-alpha-D-mannosyltransferase
The biosynthesis of asparagine-linked glycoproteins (N-linked protein glycosylation) utilizes a dolichyl diphosphate-linked glycosyl donor, which is assembled by the series of membrane-bound glycosyltransferases that comprise the dolichol pathway. ALG11 mannosyltransferase from Saccharomyces cerevisiae carries out two sequential steps in the formation of the lipid-linked core oligosaccharide, adding two mannose residues in alpha(1->2) linkages to the nascent oligosaccharide.
CAS REGISTRY NUMBER
COMMENTARY hide
74506-43-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
gmd3 mutant cannot grow at 37°C. Construction of a plasmid pREP1+/-ALG11HA for the expression of Saccharomyces cerevisiae ALG11 in Schizosaccharomyces pombe and introducing it into gmd3 mutant. The gmd3 mutant, carrying pREP+/-ALG11HA, grows at 37°C, whereas the gmd3 mutant, carrying vector pREP1 alone, does not. The defect in acid phosphatase glycosylation of gmd3 mutant is also suppressed by Saccharomyces cerevisiae ALG11. The gmd3+/alg11+ gene is a functional homologue of the Saccharomyces cerevisiae ALG11 gene
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Umeda, K.; Yoko-o, T.; Nakayama, K.; Suzuki, T.; Jigami, Y.
Schizosaccharomyces pombe gmd3(+)/alg11(+) is a functional homologue of Saccharomyces cerevisiae ALG11 which is involved in N-linked oligosaccharide synthesis
Yeast
16
1261-1271
2000
Saccharomyces cerevisiae (P53954), Saccharomyces cerevisiae, Schizosaccharomyces pombe (O74878), Schizosaccharomyces pombe
Manually annotated by BRENDA team