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Information on EC 2.3.1.92 - sinapoylglucose-malate O-sinapoyltransferase and Organism(s) Arabidopsis thaliana and UniProt Accession Q8RUW5

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Arabidopsis thaliana
UNIPROT: Q8RUW5 not found.
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The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
atsmt, sinapoylglucose:malate sinapoyltransferase, 1-o-sinapoyl-beta-glucose:l-malate sinapoyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1-O-sinapoyl-beta-glucose:L-malate sinapoyltransferase
-
sinapoylglucose:malate sinapoyltransferase
-
1-O-sinapoyl-beta-glucose:L-malate sinapoyltransferase
-
-
1-sinapoylglucose-L-malate sinapoyltransferase
-
-
-
-
SCPL 1-O-sinapoyl-beta-glucose:L-malate sinapoyltransferase
-
-
serine carboxypeptidase-like 1-O-sinapoyl-beta-glucose:L-malate sinapoyltransferase
-
-
sinapoylglucose:malate sinapoyltransferase
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
-
Acyl group transfer
SYSTEMATIC NAME
IUBMB Comments
1-O-sinapoyl-beta-D-glucose:(S)-malate O-sinapoyltransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
76095-65-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-O-sinapoyl-beta-D-glucose + L-malate
D-glucose + sinapoyl-L-malate
show the reaction diagram
-
-
-
?
1-O-sinapoyl-beta-D-glucose + L-malate
sinapoyl-L-malate + D-glucose
show the reaction diagram
-
-
-
?
1-O-sinapoyl-beta-D-glucose
1,2-di-O-sinapoyl-beta-D-glucose + D-glucose
show the reaction diagram
-
in the absence or weak binding of L-malate at pH 8.0
-
-
?
1-O-sinapoyl-beta-D-glucose + H2O
sinapic acid + D-glucose
show the reaction diagram
-
minor hydrolytic activity under standard conditions, strongly increasing in the absence of L-malate and at pH 8.0
-
-
?
1-O-sinapoyl-beta-D-glucose + L-malate
D-glucose + sinapoyl-L-malate
show the reaction diagram
1-O-sinapoyl-beta-D-glucose + L-malate
sinapoyl-L-malate + D-glucose
show the reaction diagram
-
standard conditions, pH 6.0
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
1-O-sinapoyl-beta-D-glucose + L-malate
sinapoyl-L-malate + D-glucose
show the reaction diagram
-
-
-
?
1-O-sinapoyl-beta-D-glucose + L-malate
sinapoyl-L-malate + D-glucose
show the reaction diagram
-
standard conditions, pH 6.0
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(R)-3-hydroxybutyrate
competitive inhibition of 13%
(S)-2-hydroxybutyrate
competitive inhibition of 12%
D-malate
competitive inhibition of 7%
Glutarate
competitive inhibition of 15%
succinate
competitive inhibition of 21%
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.115
1-O-sinapoyl-beta-D-glucose
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0000084
mutant R322E
0.0001092
mutant N73A
0.000126
mutant D172E/S175A
0.000168
mutant D358A
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
-
activity assay
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
activity assay
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
rosette leaf more abundant than in cauline leaf
Manually annotated by BRENDA team
-
traces
Manually annotated by BRENDA team
-
flowering stem
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
of mesophyll and epidermal cells
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
SCP8_ARATH
433
0
49439
Swiss-Prot
Secretory Pathway (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
47190
-
expressed in Escherichia coli, MALDI-TOF
52000 - 55000
-
expressed in wild-type and in Nicotiana tabacum, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
-
protein expressed in Escherichia coli has an 8 kDa lower molecular weight than wild-type
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D172E/S175A
mutant, relative activity 15%
D358A
mutant, relative activity 20%
H411A
mutant, no activity
N73A
mutant, relative activity 13%
R322E
mutant, relative activity 1%
S173A
mutant, no activity
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21DE3
expressed in Escherichia coli BL21DE3
-
expressed in Nicotiana tabacum cv. Xanthi
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Hause, B.; Meyer, K.; Viitanen, P.V.; Chapple, C.; Strack, D.
Immunolocalization of 1-O-sinapoylglucose:malate sinapoyltransferase in Arabidopsis thaliana
Planta
215
26-32
2002
Arabidopsis thaliana
Manually annotated by BRENDA team
Lehfeldt, C.; Shirley, A.M.; Meyer, K.; Ruegger, M.O.; Cusumano, J.C.; Viitanen, P.V.; Strack, D.; Chapple, C.
Cloning of the SNG1 gene of Arabidopsis reveals a role for a serine carboxypeptidase-like protein as an acyltransferase in secondary metabolism
Plant Cell
12
1295-1306
2000
Arabidopsis thaliana (Q8RUW5)
Manually annotated by BRENDA team
Mock, H.P.; Vogt, T.; Strack, D.
Sinapoylglucose: malate sinapoyltransferase activity in Arabidopsis thaliana and Brassica rapa
Z. Naturforsch. C
47c
680-682
1992
Arabidopsis thaliana, Brassica rapa
-
Manually annotated by BRENDA team
Stehle, F.; Brandt, W.; Milkowski, C.; Strack, D.
Structure determinants and substrate recognition of serine carboxypeptidase-like acyltransferases from plant secondary metabolism
FEBS Lett.
580
6366-6374
2006
Arabidopsis thaliana (Q8RUW5)
Manually annotated by BRENDA team
Fraser, C.M.; Rider, L.W.; Chapple, C.
An expression and bioinformatics analysis of the Arabidopsis serine carboxypeptidase-like gene family
Plant Physiol.
138
1136-1148
2005
Arabidopsis thaliana (Q8RUW5)
Manually annotated by BRENDA team
Stehle, F.; Brandt, W.; Schmidt, J.; Milkowski, C.; Strack, D.
Activities of Arabidopsis sinapoylglucose:malate sinapoyltransferase shed light on functional diversification of serine carboxypeptidase-like acyltransferases
Phytochemistry
69
1826-1831
2008
Arabidopsis thaliana
Manually annotated by BRENDA team
Stehle, F.; Brandt, W.; Stubbs, M.T.; Milkowski, C.; Strack, D.
Sinapoyltransferases in the light of molecular evolution
Phytochemistry
70
1652-1662
2009
Arabidopsis thaliana (Q8RUW5)
Manually annotated by BRENDA team