Literature summary for 2.3.1.92 extracted from

Stehle, F.; Brandt, W.; Stubbs, M.T.; Milkowski, C.; Strack, D.
Sinapoyltransferases in the light of molecular evolution (2009), Phytochemistry, 70, 1652-1662.

Search for more literature for 2.3.1.92

Protein Variants

Protein Variants	Comment	Organism
D172E/S175A	mutant, relative activity 15%	Arabidopsis thaliana
D358A	mutant, relative activity 20%	Arabidopsis thaliana
H411A	mutant, no activity	Arabidopsis thaliana
N73A	mutant, relative activity 13%	Arabidopsis thaliana
R322E	mutant, relative activity 1%	Arabidopsis thaliana
S173A	mutant, no activity	Arabidopsis thaliana

Inhibitors

Inhibitors	Comment	Organism	Structure
(R)-3-hydroxybutyrate	competitive inhibition of 13%	Arabidopsis thaliana
(S)-2-hydroxybutyrate	competitive inhibition of 12%	Arabidopsis thaliana
D-malate	competitive inhibition of 7%	Arabidopsis thaliana
Glutarate	competitive inhibition of 15%	Arabidopsis thaliana
succinate	competitive inhibition of 21%	Arabidopsis thaliana

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.115	-	1-O-sinapoyl-beta-D-glucose	-	Arabidopsis thaliana
0.89	-	L-malate	-	Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1-O-sinapoyl-beta-D-glucose + L-malate	Arabidopsis thaliana	-	sinapoyl-L-malate + D-glucose	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q8RUW5	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0000084	-	mutant R322E	Arabidopsis thaliana
0.0001092	-	mutant N73A	Arabidopsis thaliana
0.000126	-	mutant D172E/S175A	Arabidopsis thaliana
0.000168	-	mutant D358A	Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1-O-sinapoyl-beta-D-glucose + L-malate	-	Arabidopsis thaliana	sinapoyl-L-malate + D-glucose	-	?

Synonyms

Synonyms	Comment	Organism
1-O-sinapoyl-beta-glucose:L-malate sinapoyltransferase	-	Arabidopsis thaliana
AtSMT	-	Arabidopsis thaliana
SMT	-	Arabidopsis thaliana

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Release 2023.1 (January 2023)