The enzyme is found in some methanogenic archaea and bacteria. In archaea it is induced under salt stress. The product, N6-acetyl-β-L-lysine, serves as a compatible solute, conferring high salt resistance on the producing organisms.
The expected taxonomic range for this enzyme is: Bacteria, Archaea
The enzyme is found in some methanogenic archaea and bacteria. In archaea it is induced under salt stress. The product, N6-acetyl-beta-L-lysine, serves as a compatible solute, conferring high salt resistance on the producing organisms.
wild-type grows optimally at an NaCl concentration of 376.5 mM but tolerates NaCl concentrations up to 1 M. Growth of a mutant lacking AblA and AblB activities is indistinguishable from wild type at 376.5 mM NaCl. Growth of the mutant is severely inhibited at 800 mM NaCl and almost completely impaired at 1 M NaCl
deletion of the genes encoding lysine-2,3-aminomutase (ablA) and beta-lysine acetyltransferase (ablB) abolishes the production of N6-acetyl-beta-lysine. The mutant grows well at low and intermediate salinities. Growth at 800 mM NaCl is only slowed down but not impaired. The mutant displays an increased glutamate pool and in addition, alanine is produced up to 0.36 micromol per mg protein. The cellular alanine concentration increases with the salinity of the medium and decreases in the presence of glycine betaine in the medium
expression of the N-acetyltransferase YodP and L-lysine 2,3-aminomutase kamA genes from an isopropyl beta-D-1-thiogalactopyranoside-inducible promoter on a plasmid in Bacillus subtilis, leads to synthesis of 0.28 micromol/mg protein of Nepsilon-acetyl-beta-lysine
expression of the N-acetyltransferase YodP and L-lysine 2,3-aminomutase kamA genes from an isopropyl beta-D-1-thiogalactopyranoside-inducible promoter on a plasmid in Bacillus subtilis, leads to synthesis of 0.28 micromol/mg protein of Nepsilon-acetyl-beta-lysine
Lysine-2,3-aminomutase and beta-lysine acetyltransferase genes of methanogenic archaea are salt induced and are essential for the biosynthesis of Nepsilon-acetyl-beta-lysine and growth at high salinity
Bacterial abl-like genes: Production of the archaeal osmolyte Nepsilon-acetyl-beta-lysine by homologous overexpression of the yodP-kamA genes in Bacillus subtilis
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