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S-adenosyl-L-methionine + 1,4-diamino-2,3-dibromo-trans-but-2-ene
S-adenosyl-L-homocysteine + ?
-
-
-
-
?
S-adenosyl-L-methionine + 1,4-diamino-2-hydroxybutane
S-adenosyl-L-homocysteine + ?
-
-
-
-
?
S-adenosyl-L-methionine + 1,4-diamino-2-oxobutane
S-adenosyl-L-homocysteine + ?
-
-
-
-
?
S-adenosyl-L-methionine + 1,4-diamino-trans-but-2-ene
S-adenosyl-L-homocysteine + ?
-
-
-
-
?
S-adenosyl-L-methionine + 4-(aminomethyl)piperidine
S-adenosyl-L-homocysteine + ?
-
-
-
-
?
S-adenosyl-L-methionine + N-methylputrescine
S-adenosylhomocysteine + N,N-dimethylputrescine
-
about 8% of activity compared to putrescine
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
S-adenosyl-L-methionine + putrescine
S-adenosylhomocysteine + N-methylputrescine
-
-
-
-
?
S-adenosyl-L-methionine + trans-1,4-diamonocyclohexane
S-adenosyl-L-homocysteine + ?
-
-
-
-
?
additional information
?
-
S-adenosyl-L-methionine + putrescine

S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme is probably involved in nicotine biosynthesis
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
enzyme is probably involved in nicotine biosynthesis
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
A0PFY1;
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
best substrate
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme is probably involved in nicotine biosynthesis
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
enzyme is probably involved in nicotine biosynthesis
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme is probably involved in nicotine biosynthesis
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
enzyme is probably involved in nicotine biosynthesis
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme is probably involved in nicotine biosynthesis
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme possibly may play an additional, subtle role in alkaloid metabolsim in leaf tissue
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme is probably involved in nicotine biosynthesis
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme is probably involved in nicotine biosynthesis
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme is probably involved in nicotine biosynthesis
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
B1VK31;
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
Q2KTH4;
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
-
?
additional information

?
-
-
requirement for substrate activity: two amino groups in a trans conformation, separated by four carbon atoms
-
-
-
additional information
?
-
-
substrate with little activity: 1,4 -diamino-cis-but-2-ene, not: aliphatic monoamines, 1,3-diaminopropane, 1,5-diaminopentane, N,N`-dimethyl-1,4-diamino-trans-but-2-ene, 1,3-diamino-2-hydroxypropane, 1,3-diaminoacetone, 1,4-diaminobut-2-yne, N-carbamoylputrescine
-
-
-
additional information
?
-
inactive with n-butylamine, 1,6-diaminohexane and 1,7-diaminoheptane, the PMT enzyme of potato appears to be specific for putrescine, it does not accept other mono-, di- or tri-amines
-
-
-
additional information
?
-
-
inactive with n-butylamine, 1,6-diaminohexane and 1,7-diaminoheptane, the PMT enzyme of potato appears to be specific for putrescine, it does not accept other mono-, di- or tri-amines
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
S-adenosyl-L-methionine + putrescine

S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
Q9S7W8, Q9XJ42
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme is probably involved in nicotine biosynthesis
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
Q9S7W8, Q9XJ42
enzyme is probably involved in nicotine biosynthesis
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
Q9XJ41
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme is probably involved in nicotine biosynthesis
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
Q9XJ41
enzyme is probably involved in nicotine biosynthesis
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme is probably involved in nicotine biosynthesis
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
Q9SB10, Q9ZWT7, Q9ZWT8, Q9ZWT9
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
Q9SB10, Q9ZWT7, Q9ZWT8, Q9ZWT9
enzyme is probably involved in nicotine biosynthesis
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme is probably involved in nicotine biosynthesis
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme possibly may play an additional, subtle role in alkaloid metabolsim in leaf tissue
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme is probably involved in nicotine biosynthesis
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme is probably involved in nicotine biosynthesis
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme is probably involved in nicotine biosynthesis
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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1,6-diaminohexane
-
weaker inhibitor
1,7-Diaminoheptane
-
weaker inhibitor
2-(aminomethyl)pyridine
-
-
4-(aminomethyl)pyridine
-
-
Ca2+
-
28% inhibition by 5 mM
Cr2+
-
31% inhibition by 5 mM
Cu2+
-
73% inhibition by 5 mM
EDTA
-
23% inhibition by 5 mM
Fe2+
-
33% inhibition by 5 mM
Fe3+
-
14% inhibition by 5 mM
Mg2+
-
28% inhibition by 5 mM
Mn2+
-
26% inhibition by 5 mM
N-methyl-1,3-diaminopropane
Ni2+
-
71% inhibition by 5 mM
p-chloromercuribenzoate
-
strong
S-adenosyl-L-homocysteine
Zn2+
-
27% inhibition by 5 mM
1,3-Diaminopropane

;
1,3-Diaminopropane
A0PFY1;
-
1,3-Diaminopropane
B1VK31;
-
Ag+

-
51% inhibition by 5 mM
cadaverine

;
cadaverine
-
strong inhibitor
n-butylamine

strong inhibitor; strong inhibitor
n-butylamine
-
strong inhibitor
n-butylamine
-
strong inhibitor; strong inhibitor
n-butylamine
-
strong inhibitor
n-butylamine
-
strong inhibitor
n-butylamine
-
strong inhibitor; strong inhibitor
n-butylamine
A0PFY1;
strong inhibitor
n-butylamine
-
strong inhibitor
n-butylamine
-
strong inhibitor
n-butylamine
-
strong inhibitor
n-butylamine
strong inhibitor; strong inhibitor
n-butylamine
strong inhibitor
n-butylamine
-
strong inhibitor; strong inhibitor
n-butylamine
strong inhibitor; strong inhibitor; strong inhibitor; strong inhibitor
n-butylamine
-
strong inhibitor
n-butylamine
strong inhibitor
n-butylamine
B1VK31;
strong inhibitor
n-butylamine
-
strong inhibitor
n-butylamine
strong inhibitor
N-methyl-1,3-diaminopropane

;
N-methyl-1,3-diaminopropane
-
-
N-methyl-1,3-diaminopropane
-
;
N-methyl-1,3-diaminopropane
-
-
N-methyl-1,3-diaminopropane
-
-
N-methyl-1,3-diaminopropane
-
;
N-methyl-1,3-diaminopropane
A0PFY1;
-
N-methyl-1,3-diaminopropane
-
-
N-methyl-1,3-diaminopropane
-
-
N-methyl-1,3-diaminopropane
-
-
N-methyl-1,3-diaminopropane
;
N-methyl-1,3-diaminopropane
-
N-methyl-1,3-diaminopropane
-
;
N-methyl-1,3-diaminopropane
-
N-methyl-1,3-diaminopropane
-
-
N-methyl-1,3-diaminopropane
-
N-methyl-1,3-diaminopropane
B1VK31;
-
N-methyl-1,3-diaminopropane
-
-
N-methyl-1,3-diaminopropane
-
-
N-methylputrescine

inhibitor with approximately competitive interaction kinetics to putrescine; inhibitor with approximately competitive interaction kinetics to putrescine
N-methylputrescine
-
inhibitor with approximately competitive interaction kinetics to putrescine
N-methylputrescine
-
inhibitor with approximately competitive interaction kinetics to putrescine; inhibitor with approximately competitive interaction kinetics to putrescine
N-methylputrescine
-
inhibitor with approximately competitive interaction kinetics to putrescine
N-methylputrescine
-
inhibitor with approximately competitive interaction kinetics to putrescine
N-methylputrescine
-
inhibitor with approximately competitive interaction kinetics to putrescine; inhibitor with approximately competitive interaction kinetics to putrescine
N-methylputrescine
A0PFY1;
inhibitor with approximately competitive interaction kinetics to putrescine
N-methylputrescine
-
inhibitor with approximately competitive interaction kinetics to putrescine
N-methylputrescine
-
inhibitor with approximately competitive interaction kinetics to putrescine
N-methylputrescine
-
inhibitor with approximately competitive interaction kinetics to putrescine
N-methylputrescine
inhibitor with approximately competitive interaction kinetics to putrescine; inhibitor with approximately competitive interaction kinetics to putrescine
N-methylputrescine
inhibitor with approximately competitive interaction kinetics to putrescine
N-methylputrescine
-
inhibitor with approximately competitive interaction kinetics to putrescine; inhibitor with approximately competitive interaction kinetics to putrescine
N-methylputrescine
inhibitor with approximately competitive interaction kinetics to putrescine; inhibitor with approximately competitive interaction kinetics to putrescine; inhibitor with approximately competitive interaction kinetics to putrescine; inhibitor with approximately competitive interaction kinetics to putrescine
N-methylputrescine
-
inhibitor with approximately competitive interaction kinetics to putrescine
N-methylputrescine
inhibitor with approximately competitive interaction kinetics to putrescine
N-methylputrescine
B1VK31;
inhibitor with approximately competitive interaction kinetics to putrescine
N-methylputrescine
-
inhibitor with approximately competitive interaction kinetics to putrescine
N-methylputrescine
-
inhibitor with approximately competitive interaction kinetics to putrescine
S-adenosyl-L-homocysteine

-
competitive inhibitor
S-adenosyl-L-homocysteine
-
-
additional information

-
no enzyme activity without the addition of S-adenosyl-L-methionine
-
additional information
-
monoamines p.e. cyclohexylamine, exo-2-aminonorborane, n-butylamine, 4-methylcyclohexylamine, cyclopentylamine; not: 1,3-diaminopropane, 2-(2-aminoethyl)pyridine, 2-aminopyridine, 3-aminopyridine, 4-aminopyridine
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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AbPMT1 is expressed more strongly than ABPMT2 in the root
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AbPMT1 is expressed more strongly than ABPMT2 in the root; AbPMT1 is expressed more strongly than ABPMT2 in the root
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expressed in Escherichia coli
expressed in Escherichia coli M15[pREP4] cells
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expressed in Escherichia coli Rosetta-gami (DE3), transformed with a pET-21d vector encoding pmt
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expressed in Escherichia coli, three genes isolated from Nicotiana sylvestris: NsPMT1, NsPMT2, NsPMT3; expressed in Escherichia coli, three genes isolated from Nicotiana sylvestris: NsPMT1, NsPMT2, NsPMT3; expressed in Escherichia coli, three genes isolated from Nicotiana sylvestris: NsPMT1, NsPMT2, NsPMT3
Q9SB10;
expressed in Escherichia coli; expressed in Escherichia coli
expressed in Escherichia coli; expressed in Escherichia coli; expressed in Escherichia coli; expressed in Escherichia coli
expressed in roots and leaves
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expressed in Rosetta-gami (DE3) cells; expressed in Rosetta-gami (DE3) cells; into vector pET21d and transferred into Escherichia coli Rosetta-gami (DE3) cells; into vector pET21d and transferred into Escherichia coli Rosetta-gami (DE3) cells
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expressed in Rosetta-gami (DE3) cells; into vector pET21d and transferred into Escherichia coli Rosetta-gami (DE3) cells
expressed in xylem-tissues of roots, three genes isolated from Nicotiana sylvestris: NsPMT1, NsPMT2, NsPMT3, a highly conserved NsPMT 5'-flanking region is sufficient for tissue-specific and jasmonate-responsive expression of all three PMT genes; expressed in xylem-tissues of roots, three genes isolated from Nicotiana sylvestris: NsPMT1, NsPMT2, NsPMT3, a highly conserved NsPMT 5'-flanking region is sufficient for tissue-specific and jasmonate-responsive expression of all three PMT genes; expressed in xylem-tissues of roots, three genes isolated from Nicotiana sylvestris: NsPMT1, NsPMT2, NsPMT3, a highly conserved NsPMT 5'-flanking region is sufficient for tissue-specific and jasmonate-responsive expression of all three PMT genes
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into pETat-32a(+) vector and expressed in Escherichia coli BL21trxB(DE3)
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pmt1 and pmt2 cloned into pET-21d vector, PMT1 expressed in Escherichia coli Rosetta-gami (DE3), PMT2 expressed in Escherichia coli BL21 (DE3) and Rosetta-gami (DE3), pmt1 yields active enzymes, while pmt2 expression results in insoluble protein
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T1 progeny of transgenic Atropa belladonna, in which putrescine N-methyltransferase (EC 2.1.1.53) from Nicotiana tabacum (NtPMT) and hyoscyamine 6beta-hydroxylase (EC 1.14.11.14) from Hyoscyamus niger (HnH6H) are overexpressed, are established to investigate tropane alkaloids biosynthesis and distribution in ripe fruits, leaves, stems, primary roots and secondary roots under field conditions. Both NtPMT and HnH6H are detected at the transcriptional level in transgenic plants, whereas they are not detected in wild-type plants. Metabolically engineering Atropa belladonna based on overexpressing NtPMT and HnH6H shows a chemotype of rich scopolamine that greatly improves the commercial and pharmaceutical properties of Atropa belladonna
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tobacco pmt gene with a selectable marker (nptII) gene and the T-DNA of pRiA4 of Agrobacterium rhizogenes introduced into Hyoscyamus niger leaf explants using disarmed Agrobacterium tumefaciens C58C1 strain
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expressed in Escherichia coli

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expressed in Escherichia coli
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expressed in Escherichia coli
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expressed in Escherichia coli
A0PFY1;
expressed in Escherichia coli
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expressed in Escherichia coli
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expressed in Escherichia coli
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expressed in Escherichia coli
expressed in Escherichia coli
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expressed in Escherichia coli
expressed in Escherichia coli
B1VK31;
expressed in Escherichia coli
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expressed in Escherichia coli
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expressed in Escherichia coli; expressed in Escherichia coli

expressed in Escherichia coli; expressed in Escherichia coli
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expressed in Escherichia coli; expressed in Escherichia coli
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expressed in Escherichia coli; expressed in Escherichia coli
expressed in Escherichia coli; expressed in Escherichia coli
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expressed in Rosetta-gami (DE3) cells; into vector pET21d and transferred into Escherichia coli Rosetta-gami (DE3) cells

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expressed in Rosetta-gami (DE3) cells; into vector pET21d and transferred into Escherichia coli Rosetta-gami (DE3) cells
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expressed in Rosetta-gami (DE3) cells; into vector pET21d and transferred into Escherichia coli Rosetta-gami (DE3) cells
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expressed in Rosetta-gami (DE3) cells; into vector pET21d and transferred into Escherichia coli Rosetta-gami (DE3) cells
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expressed in Rosetta-gami (DE3) cells; into vector pET21d and transferred into Escherichia coli Rosetta-gami (DE3) cells
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tobacco pmt gene with a selectable marker (nptII) gene and the T-DNA of pRiA4 of Agrobacterium rhizogenes introduced into Hyoscyamus niger leaf explants using disarmed Agrobacterium tumefaciens C58C1 strain

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tobacco pmt gene with a selectable marker (nptII) gene and the T-DNA of pRiA4 of Agrobacterium rhizogenes introduced into Hyoscyamus niger leaf explants using disarmed Agrobacterium tumefaciens C58C1 strain
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Feth, F.; Arfmann, H.A.; Wray, V.; Wagner, K.G.
Determination of putrescine N-methyltransferase by high performance liquid chromatography
Phytochemistry
24
921-923
1985
Datura stramonium, Nicotiana tabacum
-
brenda
Mizusaki, S.; Tanabe, Y.; Noguchi, M.; Tamaki, E.
Phytochemical studies on tobacco alkaloids XIV, the occurrence
Plant Cell Physiol.
12
633-640
1971
Nicotiana tabacum
-
brenda
Walton, N.J.; Peerless, A.C.J.; Robins, R.J.; Rhodes, M.J.C.; Boswell, H.D.; Robins, D.J.
Purification and properties of putrescine N-methyltransferase from transformed roots of Datura stramonium L
Planta
193
9-15
1994
Datura stramonium
-
brenda
Hashimoto, T.; Shoji, T.; Mihara, T.; Oguri, H.; Tamaki, K.; Suzuki, K.I.; Yamada, Y.
Intraspecific variability of the tandem repeats in Nicotiana putrescine N-methyltransferases
Plant Mol. Biol.
37
25-37
1998
Atropa belladonna, Hyoscyamus niger, Nicotiana otophora, Nicotiana sylvestris, Nicotiana sylvestris (Q9SB10), Nicotiana sylvestris (Q9ZWT7), Nicotiana sylvestris (Q9ZWT8), Nicotiana sylvestris (Q9ZWT9), Nicotiana tabacum, Nicotiana tomentosiformis
brenda
Shoji, T.; Yamada, Y.; Hashimoto, T.
Jasmonate induction of putrescine N-methyltransferase genes in the root of Nicotiana sylvestris
Plant Cell Physiol.
41
831-839
2000
Nicotiana sylvestris, Nicotiana sylvestris (Q9ZWT7), Nicotiana sylvestris (Q9ZWT8), Nicotiana sylvestris (Q9ZWT9)
brenda
Sachan, N.; Falcone, D.L.
Wound-induced gene expression of putrescine N-methyltransferase in leaves of Nicotiana tabacum
Phytochemistry
61
797-805
2002
Nicotiana tabacum
brenda
Suzuki, K.I.; Yamada, Y.; Hashimoto, T.
Expression of Atropa belladonna putrescine N-methyltransferase gene in root pericycle
Plant Cell Physiol.
40
289-297
1999
Atropa belladonna, Atropa belladonna (Q9S7W8), Atropa belladonna (Q9XJ42), Hyoscyamus niger, Hyoscyamus niger (Q9XJ41)
brenda
Rothe, G.; Hachiya, A.; Yamada, Y.; Hashimoto, T.; Drager, B.
Alkaloids in plants and root cultures of Atropa belladonna overexpressing putrescine N-methyltransferase
J. Exp. Bot.
54
2065-2070
2003
Nicotiana tabacum
brenda
Zhang, L.; Ding, R.; Chai, Y.; Bonfill, M.; Moyano, E.; Oksman-Caldentey, K.M.; Xu, T.; Pi, Y.; Wang, Z.; Zhang, H.; Kai, G.; Liao, Z.; Sun, X.; Tang, K.
Engineering tropane biosynthetic pathway in Hyoscyamus niger hairy root cultures
Proc. Natl. Acad. Sci. USA
101
6786-6791
2004
Hyoscyamus niger
brenda
Teuber, M.; Azemi, M.E.; Namjoyan, F.; Meier, A.C.; Wodak, A.; Brandt, W.; Draeger, B.
Putrescine N-methyltransferases-a structure-function analysis
Plant Mol. Biol.
63
787-801
2007
Calystegia sepium, Calystegia sepium (Q2KTH3), Datura inoxia (Q2KTH1), Datura inoxia (Q2KTH2), Datura stramonium (Q70EW6), Physalis divaricata (Q2KTH0), Solanum dulcamara, Solanum lycopersicum (Q2KTH4), Solanum lycopersicum
brenda
Paschalidis, K.A.; Roubelakis-Angelakis, K.A.
Sites and regulation of polyamine catabolism in the tobacco plant. Correlations with cell division/expansion, cell cycle progression, and vascular development
Plant Physiol.
138
2174-2184
2005
Nicotiana tabacum
brenda
Stenzel, O.; Teuber, M.; Draeger, B.
Putrescine N-methyltransferase in Solanum tuberosum L., a calystegine-forming plant
Planta
223
200-212
2006
Hyoscyamus niger, Hyoscyamus niger (Q9XJ41), Solanum tuberosum (Q70AR0), Solanum tuberosum
brenda
Zhang, L.; Yang, B.; Lu, B.; Kai, G.; Wang, Z.; Xia, Y.; Ding, R.; Zhang, H.; Sun, X.; Chen, W.; Tang, K.
Tropane alkaloids production in transgenic Hyoscyamus niger hairy root cultures over-expressing putrescine N-methyltransferase is methyl jasmonate-dependent
Planta
225
887-896
2006
Hyoscyamus niger, Nicotiana tabacum
brenda
Liu, T.; Zhu, P.; Cheng, K.D.; Meng, C.; Zhu, H.X.
Molecular cloning and expression of putrescine N-methyltransferase from the hairy roots of Anisodus tanguticus
Planta Med.
71
987-989
2005
Anisodus tanguticus, Anisodus tanguticus (Q68HB1)
brenda
Biastoff, S.; Teuber, M.; Zhou, Z.S.; Draeger, B.
Colorimetric activity measurement of a recombinant putrescine N-methyltransferase from Datura stramonium
Planta Med.
72
1136-1141
2006
Datura stramonium
brenda
Biastoff, S.; Reinhardt, N.; Reva, V.; Brandt, W.; Draeger, B.
Evolution of putrescine N-methyltransferase from spermidine synthase demanded alterations in substrate binding
FEBS Lett.
583
3367-3374
2009
Datura stramonium, Datura stramonium (Q70EW6)
brenda
Kai, G.; Zhang, Y.; Chen, J.; Li, L.; Yan, X.; Zhang, R.; Liao, P.; Lu, X.; Wang, W.; Zhou, G.
Molecular characterization and expression analysis of two distinct putrescine N-methyltransferases from roots of Anisodus acutangulus
Physiol. Plant.
135
121-129
2009
Anisodus acutangulus, Anisodus acutangulus (B4YSH2), Anisodus acutangulus (B4YSH3)
brenda
Biastoff, S.; Brandt, W.; Draeger, B.
Putrescine N-methyltransferase--the start for alkaloids
Phytochemistry
70
1708-1718
2009
Anisodus acutangulus (B4YSH2), Anisodus acutangulus (B4YSH3), Anisodus tanguticus (Q68HB1), Atropa belladonna (Q9S7W8), Atropa belladonna (Q9XJ42), Calystegia sepium (Q2KTH3), Capsicum annuum, Datura inoxia (Q2KTH1), Datura inoxia (Q2KTH2), Datura metel (A0PFY1), Datura stramonium (Q70EW6), Hyoscyamus niger (Q9XJ41), Ipomoea batatas, Nicotiana attenuata (Q93XQ4), Nicotiana attenuata (Q93XQ5), Nicotiana benthamiana (B1NYI5), Nicotiana sylvestris (Q9ZWT7), Nicotiana sylvestris (Q9ZWT9), Nicotiana tabacum, Nicotiana tabacum (Q42963), Nicotiana tabacum (Q9SEH4), Nicotiana tabacum (Q9SEH5), Nicotiana tabacum (Q9SEH7), Physalis divaricata (Q2KTH0), Scopolia parviflora (Q4VQ73), Solanum dulcamara (B1VK31), Solanum lycopersicum (Q2KTH4), Solanum tuberosum (Q70AR0)
brenda
Kai, G.; Yang, S.; Luo, X.; Zhou, W.; Fu, X.; Zhang, A.; Zhang, Y.; Xiao, J.
Co-expression of AaPMT and AaTRI effectively enhances the yields of tropane alkaloids in Anisodus acutangulus hairy roots
BMC Biotechnol.
11
43
2011
Anisodus acutangulus
brenda
Kai, G.; Yang, S.; Zhang, Y.; Luo, X.; Fu, X.; Zhang, A.; Xiao, J.
Effects of different elicitors on yield of tropane alkaloids in hairy roots of Anisodus acutangulus
Mol. Biol. Rep.
39
1721-1729
2012
Anisodus acutangulus
brenda
Sachan, N.; Rogers, D.T.; Yun, K.Y.; Littleton, J.M.; Falcone, D.L.
Reactive oxygen species regulate alkaloid metabolism in undifferentiated N. tabacum cells
Plant Cell Rep.
29
437-448
2010
Nicotiana tabacum
brenda
Yang, C.; Chen, M.; Zeng, L.; Zhang, L.; Liu, X.; Lan, X.; Tang, K.; Liao, Z.
Improvement of tropane alkaloids production in hairy root cultures of Atropa belladonna by overexpressing pmt and h6h genes
Plant OMICS
4
29-33
2011
Atropa belladonna
-
brenda
Junker, A.; Fischer, J.; Sichhart, Y.; Brandt, W.; Draeger, B.
Evolution of the key alkaloid enzyme putrescine N-methyltransferase from spermidine synthase
Front. Plant Sci.
4
260
2013
Datura stramonium, Datura stramonium (Q70EW6)
brenda
Xia, K.; Liu, X.; Zhang, Q.; Qiang, W.; Guo, J.; Lan, X.; Chen, M.; Liao, Z.
Promoting scopolamine biosynthesis in transgenic Atropa belladonna plants with pmt and h6h overexpression under field conditions
Plant Physiol. Biochem.
106
46-53
2016
Nicotiana tabacum
brenda