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EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Commentary	Reference
2.1.1.53	0.013	-	S-adenosyl-L-methionine	wild-type, pH 8.0, 37°C	733916
2.1.1.53	0.042	-	S-adenosyl-L-methionine	-	706130
2.1.1.53	0.0561	-	S-adenosyl-L-methionine	wild type enzyme, in 20 mM HEPES, pH 8.0, 2 mM dithiothreitol, 1 mM ascorbic acid, at 37°C	703724
2.1.1.53	0.0758	-	putrescine	SPDS-PMT chimeric enzyme consisting of 144 N-terminal amino acids of spermidine synthase and 162 C-terminal amino acids of putrescine N-methyltransferase, in 20 mM HEPES, pH 8.0, 2 mM dithiothreitol, 1 mM ascorbic acid, at 37°C	703724
2.1.1.53	0.099	-	S-adenosyl-L-methionine	mutant H108L, pH 8.0, 37°C	733916
2.1.1.53	0.1	-	S-adenosyl-L-methionine	-	485445, 706130
2.1.1.53	0.108	-	putrescine	-	706130
2.1.1.53	0.11	-	S-adenosyl-L-methionine	-	485444
2.1.1.53	0.113	-	putrescine	-	676557
2.1.1.53	0.12	-	putrescine	isoenzyme PMT1	676557

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Release 2023.1 (January 2023)