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Information on EC 2.1.1.280 - selenocysteine Se-methyltransferase and Organism(s) Escherichia coli and UniProt Accession Q47690

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EC Tree
     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.280 selenocysteine Se-methyltransferase
IUBMB Comments
The enzyme uses S-adenosyl-L-methionine as methyl donor less actively than S-methyl-L-methionine. The enzyme from broccoli (Brassica oleracea var. italica) also has the activity of EC 2.1.1.10, homocysteine S-methyltransferase .
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This record set is specific for:
Escherichia coli
UNIPROT: Q47690
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The taxonomic range for the selected organisms is: Escherichia coli
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
selenocysteine methyltransferase, bosmt, bjsmt, secmt, selenocysteine se-methyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Hcy methyltransferase
-
YagD protein
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
S-methyl-L-methionine:L-selenocysteine Se-methyltransferase
The enzyme uses S-adenosyl-L-methionine as methyl donor less actively than S-methyl-L-methionine. The enzyme from broccoli (Brassica oleracea var. italica) also has the activity of EC 2.1.1.10, homocysteine S-methyltransferase [4].
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 4-thiobutyric acid
S-adenosyl-L-homocysteine + 4-methylthiobutyric acid
show the reaction diagram
0.014% relative activity compared to activity with L-homocysteine set at 100%
-
-
?
S-adenosyl-L-methionine + D-homocysteine
?
show the reaction diagram
16% relative activity compared to activity with L-homocysteine set at 100%
-
-
?
S-adenosyl-L-methionine + DL-homocysteine
?
show the reaction diagram
93% relative activity compared to activity with L-homocysteine set at 100%
-
-
?
S-adenosyl-L-methionine + DL-selenohomocysteine
?
show the reaction diagram
213% relative activity compared to activity with L-homocysteine set at 100%
-
-
?
S-adenosyl-L-methionine + L-cysteine
S-adenosyl-L-homocysteine + S-methyl-L-cysteine
show the reaction diagram
less than 0.003% relative activity compared to activity with L-homocysteine set at 100%
-
-
?
S-adenosyl-L-methionine + L-homocysteine
?
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + L-homoserine
?
show the reaction diagram
less than 0.003% relative activity compared to activity with L-homocysteine set at 100%
-
-
?
S-adenosyl-L-methionine + L-selenocysteine
S-adenosyl-L-homocysteine + Se-methyl-L-selenocysteine
show the reaction diagram
S-adenosyl-L-methionine + L-selenohomocysteine
?
show the reaction diagram
-
-
-
?
S-methyl-L-methionine + L-selenocysteine
L-methionine + Se-methyl-L-selenocysteine
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-methyl-L-methionine + L-selenocysteine
L-methionine + Se-methyl-L-selenocysteine
show the reaction diagram
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
L-methionine
product inhibition above 0.5 mM
S-adenosyl-L-methionine
product inhibition above 0.25 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.043
DL-selenohomocysteine
0.045
L-homocysteine
app. Km-value with [methyl-14C]S-adenosyl-L-methionine, pH 6.5, 30°C
0.59
L-selenocysteine
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.74
DL-selenohomocysteine
0.38
L-homocysteine
with [methyl-14C]S-adenosyl-L-methionine, pH 6.5, 30°C
0.00017
L-selenocysteine
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
17
DL-selenohomocysteine
with [methyl-14C]S-adenosyl-L-methionine, pH 6.5, 30°C
8.4
L-homocysteine
with [methyl-14C]S-adenosyl-L-methionine, pH 6.5, 30°C
0.00029
L-selenocysteine
with [methyl-14C]S-adenosyl-L-methionine, pH 6.5, 30°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
detoxification of selenium-containing compounds
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22500
1 * 22500, calculated from sequence
33500
calculated from gene sequence
34000
gel filtration
35000
SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by ammonium sulfate precipitation, hydrophobic interaction chromatography and anion exchange chromatography with Q-Sepharose
recombinant enzyme
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned into plasmid pT7-7 and overexpression in Escherichia coli BL21(DE3) cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Neuhierl, B.; Thanbichler, M.; Lottspeich, F.; Bck, A.
A family of S-methylmethionine-dependent thiol/selenol methyltransferases. Role in selenium tolerance and evolutionary relation
J. Biol. Chem.
274
5407-5414
1999
Astragalus bisulcatus, Astragalus bisulcatus (P56707), Escherichia coli (Q47690), Escherichia coli
Manually annotated by BRENDA team