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Literature summary for 2.1.1.280 extracted from

  • Neuhierl, B.; Thanbichler, M.; Lottspeich, F.; Böck, A.
    A family of S-methylmethionine-dependent thiol/selenol methyltransferases. Role in selenium tolerance and evolutionary relation (1999), J. Biol. Chem., 274, 5407-5414.
    View publication on PubMed
Search for more literature for 2.1.1.280

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli
-
 Astragalus bisulcatus
cloned into plasmid pT7-7 and overexpression in Escherichia coli BL21(DE3) cells Escherichia coli
pUKE vector used for cloning of cDNA sequence coding for the SeCys-methyltransferase from Astragalus bisulcatus in Escherichia coli Astragalus bisulcatus

Inhibitors

Inhibitors Comment Organism Structure
L-methionine product inhibition above 0.5 mM Escherichia coli
S-adenosyl-L-methionine product inhibition above 0.25 mM Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.043
-
 DL-selenohomocysteine pH 6, 30°C, cosubstrate: S-adenosyl-L-methionine Escherichia coli
0.043
-
 DL-selenohomocysteine app. Km-value with [methyl-14C]S-adenosyl-L-methionine, pH 6.5, 30°C Escherichia coli
0.045
-
 L-homocysteine app. Km-value with [methyl-14C]S-adenosyl-L-methionine, pH 6.5, 30°C Escherichia coli
0.35
-
 DL-selenohomocysteine pH 6, 30°C, cosubstrate: S-adenosyl-L-methionine Astragalus bisulcatus
0.35
-
 DL-selenohomocysteine app. Km-value with [methyl-14C]S-adenosyl-L-methionine, pH 6.0, 30°C Astragalus bisulcatus
0.59
-
 L-selenocysteine pH 6, 30°C, cosubstrate: S-adenosyl-L-methionine Escherichia coli
0.59
-
 L-selenocysteine app. Km-valuen with [methyl-14C]S-adenosyl-L-methionine, pH 6.5, 30°C Escherichia coli
0.7
-
 L-selenocysteine pH 6, 30°C, cosubstrate: S-adenosyl-L-methionine Astragalus bisulcatus
0.7
-
 L-selenocysteine app. Km-value with [methyl-14C]S-adenosyl-L-methionine, pH 6.0, 30°C Astragalus bisulcatus

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Astragalus bisulcatus 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22500
-
 1 * 22500, calculated from sequence Escherichia coli
33500
-
 calculated from gene sequence Escherichia coli
34000
-
 gel filtration Escherichia coli
35000
-
 SDS-PAGE Escherichia coli
36700
-
 calculated from nucleotide sequence Astragalus bisulcatus
36700
-
 x * 36700, calculated from sequence Astragalus bisulcatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-methyl-L-methionine + L-selenocysteine Escherichia coli
-
 L-methionine + Se-methyl-L-selenocysteine
-
 ?
S-methyl-L-methionine + L-selenocysteine Escherichia coli S-methylmethionine is the physiological methyl group donor L-methionine + Se-methyl-L-selenocysteine
-
 ?
S-methyl-L-methionine + L-selenocysteine Astragalus bisulcatus S-methylmethionine is the physiological methyl group donor. The enzyme is involved in detoxification of selenium in selenium-accumulating plants L-methionine + Se-methyl-L-selenocysteine
-
 ?

Organism

Organism UniProt Comment Textmining
Astragalus bisulcatus
-
-
-
Astragalus bisulcatus P56707
-
-
Escherichia coli Q47690
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Astragalus bisulcatus
by ammonium sulfate precipitation, hydrophobic interaction chromatography and anion exchange chromatography with Q-Sepharose Astragalus bisulcatus
by ammonium sulfate precipitation, hydrophobic interaction chromatography and anion exchange chromatography with Q-Sepharose Escherichia coli
recombinant enzyme Escherichia coli

Source Tissue

Source Tissue Comment Organism Textmining
cell culture cell cultures contain the maximal level of selenocysteine methyltransferase under conditions of exponential growth. Stationary phase cells possess a much lower amount Astragalus bisulcatus
-
cotyledon of 4-8-week-old seedlings Astragalus bisulcatus
-
cotyledon immunoblotting analysis Astragalus bisulcatus
-
hypocotyl of 4-8-week-old seedlings Astragalus bisulcatus
-
hypocotyl immunoblotting analysis Astragalus bisulcatus
-
leaf primary of 4-8-week-old seedlings Astragalus bisulcatus
-
primary leaf immunoblotting analysis Astragalus bisulcatus
-
root of 4-8-week-old seedlings Astragalus bisulcatus
-
root immunoblotting analysis Astragalus bisulcatus
-
seedling
-
 Astragalus bisulcatus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 with L-S-methylmethionine, maximal conversion is 100%, pH 6.0, 30°C Astragalus bisulcatus
0.016
-
 with [methyl-14C]S-adenosyl-L-methionine, maximal conversion is 10%, pH 6.0, 30°C Astragalus bisulcatus
0.68
-
 with S-adenosyl-L-methionine, maximal conversion is 20-25%, pH 6.0, 30°C Astragalus bisulcatus
1.5
-
 with DL-S-methylmethionine, maximal conversion is 50%, pH 6.0, 30°C Astragalus bisulcatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme also catalyzes the reaction of EC 2.1.1.10 Escherichia coli ?
-
 ?
additional information no activity with trimethylsulfonium and glycine betaine as methyl donor Astragalus bisulcatus ?
-
 ?
additional information no activity with trimethylsulfonium and glycine betaine as methyl donor Escherichia coli ?
-
 ?
S-adenosyl-L-methionine + 4-thiobutyric acid 0.014% relative activity compared to activity with L-homocysteine set at 100% Escherichia coli S-adenosyl-L-homocysteine + 4-methylthiobutyric acid
-
 ?
S-adenosyl-L-methionine + D-homocysteine 16% relative activity compared to activity with L-homocysteine set at 100% Escherichia coli ?
-
 ?
S-adenosyl-L-methionine + DL-homocysteine 93% relative activity compared to activity with L-homocysteine set at 100% Escherichia coli ?
-
 ?
S-adenosyl-L-methionine + DL-selenohomocysteine 213% relative activity compared to activity with L-homocysteine set at 100% Escherichia coli ?
-
 ?
S-adenosyl-L-methionine + L-cysteine less than 0.003% relative activity compared to activity with L-homocysteine set at 100% Escherichia coli S-adenosyl-L-homocysteine + S-methyl-L-cysteine
-
 ?
S-adenosyl-L-methionine + L-homocysteine
-
 Escherichia coli ?
-
 ?
S-adenosyl-L-methionine + L-homoserine less than 0.003% relative activity compared to activity with L-homocysteine set at 100% Escherichia coli ?
-
 ?
S-adenosyl-L-methionine + L-selenocysteine
-
 Astragalus bisulcatus S-adenosyl-L-homocysteine + Se-methyl-L-selenocysteine
-
 ?
S-adenosyl-L-methionine + L-selenocysteine
-
 Escherichia coli S-adenosyl-L-homocysteine + Se-methyl-L-selenocysteine
-
 ?
S-adenosyl-L-methionine + L-selenocysteine S-adenosyl-L-methionine at intracellular concentrations cannot serve as an effective substrate for the enzyme Astragalus bisulcatus S-adenosyl-L-homocysteine + Se-methyl-L-selenocysteine
-
 ?
S-adenosyl-L-methionine + L-selenocysteine 0.027% relative activity compared to activity with L-homocysteine set at 100% Escherichia coli S-adenosyl-L-homocysteine + Se-methyl-L-selenocysteine
-
 ?
S-adenosyl-L-methionine + L-selenohomocysteine
-
 Escherichia coli ?
-
 ?
S-methyl-L-methionine + DL-selenohomocysteine
-
 Astragalus bisulcatus ?
-
 ?
S-methyl-L-methionine + L-selenocysteine
-
 Astragalus bisulcatus L-methionine + Se-methyl-L-selenocysteine
-
 ?
S-methyl-L-methionine + L-selenocysteine
-
 Escherichia coli L-methionine + Se-methyl-L-selenocysteine
-
 ?
S-methyl-L-methionine + L-selenocysteine S-methylmethionine is the physiological methyl group donor Escherichia coli L-methionine + Se-methyl-L-selenocysteine
-
 ?
S-methyl-L-methionine + L-selenocysteine S-methylmethionine is the physiological methyl group donor. The enzyme is involved in detoxification of selenium in selenium-accumulating plants Astragalus bisulcatus L-methionine + Se-methyl-L-selenocysteine
-
 ?
S-methyl-L-methionine + L-selenocysteine activity with S-methyl-L-methionine is higher than with S-adenosyl-L-methionine Escherichia coli L-methionine + Se-methyl-L-selenocysteine
-
 ?
S-methyl-L-methionine + L-selenocysteine activity with S-methyl-L-methionine is higher than with S-adenosyl-L-methionine Astragalus bisulcatus L-methionine + Se-methyl-L-selenocysteine
-
 ?

Subunits

Subunits Comment Organism
? x * 36700, calculated from sequence Astragalus bisulcatus
monomer 1 * 35000, SDS-PAGE Escherichia coli
monomer 1 * 22500, calculated from sequence Escherichia coli

Synonyms

Synonyms Comment Organism
Hcy methyltransferase
-
 Escherichia coli
SeCys-methyltransferase
-
 Astragalus bisulcatus
SeCys-MT
-
 Astragalus bisulcatus
selenocysteine methyltransferase
-
 Astragalus bisulcatus
smtA
-
 Astragalus bisulcatus
YagD
-
 Escherichia coli
YagD protein
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00017
-
 L-selenocysteine pH 6, 30°C, cosubstrate: S-adenosyl-L-methionine Escherichia coli
0.00017
-
 L-selenocysteine with [methyl-14C]S-adenosyl-L-methionine, pH 6.5, 30°C Escherichia coli
0.0062
-
 L-selenocysteine pH 6, 30°C, cosubstrate: S-adenosyl-L-methionine Astragalus bisulcatus
0.0062
-
 L-selenocysteine with [methyl-14C]S-adenosyl-L-methionine, pH 6.0, 30°C Astragalus bisulcatus
0.0074
-
 DL-selenohomocysteine pH 6, 30°C, cosubstrate: S-adenosyl-L-methionine Astragalus bisulcatus
0.0074
-
 DL-selenohomocysteine with [methyl-14C]S-adenosyl-L-methionine, pH 6.0, 30°C Astragalus bisulcatus
0.38
-
 L-homocysteine with [methyl-14C]S-adenosyl-L-methionine, pH 6.5, 30°C Escherichia coli
0.74
-
 DL-selenohomocysteine pH 6, 30°C, cosubstrate: S-adenosyl-L-methionine Escherichia coli
0.74
-
 DL-selenohomocysteine with [methyl-14C]S-adenosyl-L-methionine, pH 6.5, 30°C Escherichia coli

General Information

General Information Comment Organism
metabolism detoxification of selenium-containing compounds Astragalus bisulcatus
metabolism detoxification of selenium-containing compounds Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00029
-
 L-selenocysteine with [methyl-14C]S-adenosyl-L-methionine, pH 6.5, 30°C Escherichia coli
0.0089
-
 L-selenocysteine with [methyl-14C]S-adenosyl-L-methionine, pH 6.0, 30°C Astragalus bisulcatus
0.021
-
 DL-selenohomocysteine with [methyl-14C]S-adenosyl-L-methionine, pH 6.0, 30°C Astragalus bisulcatus
8.4
-
 L-homocysteine with [methyl-14C]S-adenosyl-L-methionine, pH 6.5, 30°C Escherichia coli
17
-
 DL-selenohomocysteine with [methyl-14C]S-adenosyl-L-methionine, pH 6.5, 30°C Escherichia coli