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2 S-adenosyl-L-methionine + C30 botryococcene = 2 S-adenosyl-L-homocysteine + 3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene
overall reaction
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S-adenosyl-L-methionine + 20-methyl-21,22-didehydro-20,21-dihydrobotryococcene = S-adenosyl-L-homocysteine + 3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene
(2b)
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S-adenosyl-L-methionine + 3-methyl-1,2-didehydro-2,3-dihydrobotryococcene = S-adenosyl-L-homocysteine + 3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene
(1b)
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S-adenosyl-L-methionine + C30 botryococcene = S-adenosyl-L-homocysteine + 20-methyl-21,22-didehydro-20,21-dihydrobotryococcene
(2a)
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S-adenosyl-L-methionine + C30 botryococcene = S-adenosyl-L-homocysteine + 3-methyl-1,2-didehydro-2,3-dihydrobotryococcene
(1a)
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2 S-adenosyl-L-methionine + C30 botryococcene
2 S-adenosyl-L-homocysteine + 3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene
S-adenosyl-L-methionine + 20-methyl-21,22-didehydro-20,21-dihydrobotryococcene
S-adenosyl-L-homocysteine + 3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene
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Substrates: -
Products: -
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S-adenosyl-L-methionine + 3-methyl-1,2-didehydro-2,3-dihydrobotryococcene
S-adenosyl-L-homocysteine + 3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene
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Substrates: -
Products: -
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S-adenosyl-L-methionine + C30 botryococcene
S-adenosyl-L-homocysteine + 20-methyl-21,22-didehydro-20,21-dihydrobotryococcene
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Substrates: -
Products: -
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S-adenosyl-L-methionine + C30 botryococcene
S-adenosyl-L-homocysteine + 3-methyl-1,2-didehydro-2,3-dihydrobotryococcene
additional information
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Substrates: mono- and dimethylated products with terminal carbons C-22/C-20 as the atomic acceptor sites for the methyl additions to botryococcene, NMR analysis, overview. TMT-3 shows only low activity with squalene
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2 S-adenosyl-L-methionine + C30 botryococcene

2 S-adenosyl-L-homocysteine + 3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene
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Substrates: overall reaction
Products: -
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2 S-adenosyl-L-methionine + C30 botryococcene
2 S-adenosyl-L-homocysteine + 3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene
-
Substrates: overall reaction
Products: -
ir
S-adenosyl-L-methionine + C30 botryococcene

S-adenosyl-L-homocysteine + 3-methyl-1,2-didehydro-2,3-dihydrobotryococcene
-
Substrates: -
Products: -
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S-adenosyl-L-methionine + C30 botryococcene
S-adenosyl-L-homocysteine + 3-methyl-1,2-didehydro-2,3-dihydrobotryococcene
-
Substrates: -
Products: -
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2 S-adenosyl-L-methionine + C30 botryococcene
2 S-adenosyl-L-homocysteine + 3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene
S-adenosyl-L-methionine + 20-methyl-21,22-didehydro-20,21-dihydrobotryococcene
S-adenosyl-L-homocysteine + 3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene
-
Substrates: -
Products: -
?
S-adenosyl-L-methionine + 3-methyl-1,2-didehydro-2,3-dihydrobotryococcene
S-adenosyl-L-homocysteine + 3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene
-
Substrates: -
Products: -
?
S-adenosyl-L-methionine + C30 botryococcene
S-adenosyl-L-homocysteine + 20-methyl-21,22-didehydro-20,21-dihydrobotryococcene
-
Substrates: -
Products: -
?
S-adenosyl-L-methionine + C30 botryococcene
S-adenosyl-L-homocysteine + 3-methyl-1,2-didehydro-2,3-dihydrobotryococcene
2 S-adenosyl-L-methionine + C30 botryococcene

2 S-adenosyl-L-homocysteine + 3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene
-
Substrates: overall reaction
Products: -
ir
2 S-adenosyl-L-methionine + C30 botryococcene
2 S-adenosyl-L-homocysteine + 3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene
-
Substrates: overall reaction
Products: -
ir
S-adenosyl-L-methionine + C30 botryococcene

S-adenosyl-L-homocysteine + 3-methyl-1,2-didehydro-2,3-dihydrobotryococcene
-
Substrates: -
Products: -
?
S-adenosyl-L-methionine + C30 botryococcene
S-adenosyl-L-homocysteine + 3-methyl-1,2-didehydro-2,3-dihydrobotryococcene
-
Substrates: -
Products: -
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Niehaus, T.D.; Kinison, S.; Okada, S.; Yeo, Y.S.; Bell, S.A.; Cui, P.; Devarenne, T.P.; Chappell, J.
Functional identification of triterpene methyltransferases from Botryococcus braunii race B
J. Biol. Chem.
287
8163-8173
2012
Botryococcus braunii, Botryococcus braunii race B
brenda